ShEEP Request for Cytek Aurora Spectral Flow Cytometer

通过这一申请，我们要求ORD共享设备评估计划（ShEEP）提供资金， 购买一台新的最先进的多色流式细胞仪，以支持、增强和实现 在爱荷华州市VA的一组多个VA资助的研究人员的专业技术需求。具体地说， 我们寻求获得Cytek Aurora光谱流式细胞仪，该仪器将安装在VA租赁的空间内， 方便地访问整个用户组。我们的研究组合是多样化的，包括传染性疾病的研究 疾病（利什曼原虫、弗朗西斯菌、H.幽门螺杆菌）、自身免疫性疾病（包括神经炎性疾病和 炎症性肠病），移植物抗宿主病，肥胖诱导的高血压的神经机制 以及白血病干细胞和B细胞淋巴瘤发生的基础研究。所有这些地区都有 对退伍军人的健康产生重大影响。重要的是，所有这些研究途径都严格依赖于 多参数流式细胞术用于评价机制生物学的各个方面。这需要 拟议的文书，以便能够获得和加强对正在处理的问题的科学见解， 正在进行的项目。随着我们对这些疾病情况的了解越来越多， 细胞群不是同质的，需要作为不同的亚群进行研究， 了解它们的确切作用或在治疗环境中调节它们的功能。几种功能测定 现在可以通过流式细胞术技术进行，其具有优于传统方法的明显优势。 这些应用正在改变我们对免疫系统中各种细胞类型之间相互作用的理解。 系统此外，还可以通过以下方式实现对多参数数据的强大利用和分析 生物信息学收购5激光，24+色Cytek Aurora流式细胞仪将提供尖端的 这将极大地增强整个用户群的研究。我们在座的 我们建议的理由和理由，列举各种具体例子，说明文书将如何提供 更深入地了解正在进行的VA资助的研究，并概述我们的坚定承诺，这将使顺利 这个方案的实施。因此，收购Cytek Aurora将有助于确保我们保持 领导人在我们各自的领域在研究的前沿，以改善退伍军人的健康。

With this application we request funds from the ORD Shared Equipment Evaluation Program (ShEEP) to purchase a new state-of-the-art multichromatic flow cytometer in order to support, enhance and fulfill the specialized technologic needs of a group of multiple VA-funded researchers at the Iowa City VA. Specifically, we seek to obtain the Cytek Aurora Spectral Flow Cytometer, which will be housed in VA-leased space, with convenient access to the entire user group. Our research portfolio is diverse and includes studies of infectious diseases (Leishmania, Francisella, H. pylori), autoimmune diseases (including neuroinflammatory disease and inflammatory bowel disease), graft-vs-host disease, neuronal mechanisms of obesity-induced hypertension and fundamental study of leukemic stem cells as well as genesis of B-cell lymphoma. All these areas have major impact for the health of veterans. Importantly, all these avenues of research critically rely on multiparameter flow cytometry for evaluation of various aspects of mechanistic biology. This requires the proposed instrument to be able to gain and enhance scientific insight into the questions being addressed in ongoing projects. As our understanding of these disease situations grows, it is becoming increasingly clear that populations of cells are not homogeneous and need to be studied as distinct subpopulations in order to understand their precise role or to modulate their function in a therapeutic setting. Several functional assays can now be performed by flow cytometric technology with a distinct advantage over traditional methodologies. These applications are changing our understanding of the interplay between various cell types of the immune system. In addition, powerful utilization and analysis of multiparametric data can be attained through bioinformatics. The acquisition of the 5-laser, 24+-color Cytek Aurora flow cytometer will provide cutting-edge instrumentation that will dramatically enhance research across the user group. We present here the justification and rationale for our proposal, cite various specific examples of how the instrument would provide greater insight into ongoing VA-funded research and outline our strong commitment that will enable the smooth implementation of this program. Thus, the acquisition of the Cytek Aurora will help ensure that we remain leaders in our respective fields at the forefront of research to improve Veteran’s health.