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Roles of strigolactones in rice responses to salinity and arsenic toxicity

独脚金内酯在水稻对盐度和砷毒性反应中的作用

基本信息

批准号：
16F16091
负责人：
TRAN LamSon・P
金额：
$ 1.47万
依托单位：
Institute of Physical and Chemical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2016
资助国家：
日本
起止时间：
2016-10-07 至 2019-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16F16091/
关键词：
strigolactones abiotic stress gene expression antioxidant mechanisms Karrikins salt tolerance metabolomics hormone homeostasis Phytohormones Abiotic stress Strigolactones Abiotic stress tolerance Oxidative stress Metal toxicity /

项目摘要

1.The results on arsenic (As) in the roots and shoots of As-treated WT and strigolactone (SL)-deficient mutants d10 and d17 using ICP-MS suggest that SL-deficient mutants accumulate more As, especially in the roots compared with WT.2.Various biochemical parameters linked to oxidative stress, membrane integrity, photosynthesis, non-enzymatic and enzymatic antioxidants in As-exposed WT and SL -deficient mutants implied that SL-mutant plants were more compromised at biochemical levels to fight against As-toxicity in comparison with WT.3.qRT PCR results of several genes involved in antioxidant system and glutathione metabolism suggest that As stress significantly upregulated the transcripts of most of the studied genes in WT plants when compared mutant plants.4.The obtained data on metabolomics, lipid profiling and hormone levels in WT and SL -deficient mutants suggest that many metabolites, including lipids involved in important biochemical pathways, as well as hormonal levels, particularly in roots were significantly upregulated in WT under As stress when compared with those in d10 and d17.5.The data on the roles of karrikins in salt adaptation clearly indicate that Kai2 mutants grown under salt stress exhibited severe growth defects when compared with WT. The other physiological, biochemical and molecular data are currently under analysis.6.Meanwhile, we have published 3 articles, including 2 review articles in the FY 2018 that are listed in the below section.

1.利用ICP-MS对砷处理的野生型和独脚金内酯（SL）缺失突变体d10和d17的根和地上部中砷（As）的分析结果表明，SL缺失突变体比野生型积累更多的As，尤其是在根中。非酶和酶抗氧化剂在作为暴露WT和SL -缺陷突变体暗示SL-突变体植物更妥协，在生化水平，以打击作为-与WT相比毒性。3.抗氧化系统和谷胱甘肽代谢相关基因的qRT PCR结果表明，与突变体植物相比，As胁迫显著上调了WT植物中大多数研究基因的转录。4.获得的代谢组学数据，WT和SL缺陷突变体的脂质谱和激素水平表明，许多代谢物，包括参与重要生化途径的脂质，以及激素水平，特别是在根中，当与d10和d17相比时，WT在As胁迫下显著上调。关于karrikins在盐适应中的作用的数据清楚地表明，盐胁迫下生长的Kai 2突变体与WT相比表现出严重的生长缺陷。其他生理、生化和分子数据目前正在分析中。6.同时，我们在2018财年发表了3篇文章，包括2篇综述文章，如下所列。