3D fabrication of biomaterial constructs for tissue engineering

用于组织工程的生物材料结构的 3D 制造

• 批准号: 17F17722
• 负责人: 福田 淳二
• 金额: $ 1.41万
• 依托单位: Yokohama National University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2017
• 资助国家: 日本
• 起止时间: 2017-11-10 至 2020-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17F17722/
• 关键词: Bioprinter; hydrogel; tissue engineering; 毛細血管; ３Dプリンタ; バイオインク; bioprinter; bioink; electrochemistry

The capability and protocol of a lab-made 3D bioprinter were tested. Cellulose-based materials have been shown to have great potential for biomedical applications in the construction of extracellular matrix-mimicking scaffolds owing to their intrinsic characteristics, such as biocompatibility and tunable 3D architecture. Herein the nanocellulose was processed from wood-based cellulose to generate nano-fibers with 100nm in length and 5nm in diameter. Using the 3D bioprinter, nanocellulose scaffolds were successfully printed. Fibroblast 3T3 cells were cultured with the printed nanocellulose scaffolds, and cells showed good adhesion and proliferation behavior.Gelatin methacrylate (GelMA) has been widely used as scaffold material for 3D bioprinting. However, one of the obstacles in using GelMA for 3D bioprinting is the photo-crosslinking speed. Without fast photo-crosslinking speed, GelMA cannot form structures with desired resolution during 3D bioprinting. Key to solve this challenge is to increase the speed of GelMA bio-ink photo-crosslinking reactions. It is shown that instead of methacrylation of gelatin, its modification with the acrylate functional group can demonstrate a higher photo-crosslinking rate. We demonstrated that the modification efficiency increased to 90% with 4:1 reagent ratio, compared to 67% for the degree of GelMA modification. The synthesized GelAc was then examined with human umbilical vein endothelial cells, which showed tubular network formation after 5 days culture, demonstrating good biocompatibility of GelAc hydrogel.

对实验室制造的 3D 生物打印机的功能和协议进行了测试。由于其固有的特性，例如生物相容性和可调节的 3D 结构，纤维素基材料已被证明在构建细胞外基质模拟支架方面具有巨大的生物医学应用潜力。这里的纳米纤维素是由木质纤维素加工而成的，长度为100纳米，直径为5纳米的纳米纤维。使用3D生物打印机，成功打印了纳米纤维素支架。用打印的纳米纤维素支架培养成纤维细胞3T3细胞，细胞表现出良好的粘附和增殖行为。甲基丙烯酸明胶（GelMA）已广泛用作3D生物打印的支架材料。然而，使用 GelMA 进行 3D 生物打印的障碍之一是光交联速度。如果没有快速的光交联速度，GelMA 就无法在 3D 生物打印过程中形成具有所需分辨率的结构。解决这一挑战的关键是提高 GelMA 生物墨水光交联反应的速度。结果表明，用丙烯酸酯官能团对明胶进行改性，而不是对明胶进行甲基丙烯酸化，可以表现出更高的光交联速率。我们证明，试剂比例为 4:1 时，修饰效率提高至 90%，而 GelMA 修饰程度为 67%。然后用人脐静脉内皮细胞检测合成的 GelAc，培养 5 天后显示管状网络形成，证明 GelAc 水凝胶具有良好的生物相容性。

项目成果

Ascorbic Acid-2-phosphate protective effect against ROS on pancreatic β-cell sphe-roids

2-磷酸抗坏血酸对胰腺β细胞球体ROS的保护作用

• 发表时间: 2018
• 作者: Dina Myasnikova;Binbin Zhang;Junji Fukuda
• 通讯作者: Junji Fukuda

Minami Masumoto, Tatsuya Osaki, Tatsuto Kageyama, Binbin Zhang, Isao Sakane, Shintaro Takahashi, Shinichi Takimoto & Junji Fukuda

增本南、大崎达也、影山辰人、张彬彬、坂根功、高桥慎太郎、泷本真一

• 发表时间: 2019
• 作者: M. Julien;P. Hohener;R. J. Robins;J. Parinet and G. S. Remaud;Cell Morphology Monitoring during Culture for Daily Cell Quality Control
• 通讯作者: Cell Morphology Monitoring during Culture for Daily Cell Quality Control