3D fabrication of biomaterial constructs for tissue engineering

用于组织工程的生物材料结构的 3D 制造

• 批准号: 17F17722
• 负责人: 福田 淳二
• 金额: $ 1.41万
• 依托单位: Yokohama National University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2017
• 资助国家: 日本
• 起止时间: 2017-11-10 至 2020-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17F17722/
• 关键词: Bioprinter; hydrogel; tissue engineering; 毛細血管; ３Dプリンタ; バイオインク; bioprinter; bioink; electrochemistry

The capability and protocol of a lab-made 3D bioprinter were tested. Cellulose-based materials have been shown to have great potential for biomedical applications in the construction of extracellular matrix-mimicking scaffolds owing to their intrinsic characteristics, such as biocompatibility and tunable 3D architecture. Herein the nanocellulose was processed from wood-based cellulose to generate nano-fibers with 100nm in length and 5nm in diameter. Using the 3D bioprinter, nanocellulose scaffolds were successfully printed. Fibroblast 3T3 cells were cultured with the printed nanocellulose scaffolds, and cells showed good adhesion and proliferation behavior.Gelatin methacrylate (GelMA) has been widely used as scaffold material for 3D bioprinting. However, one of the obstacles in using GelMA for 3D bioprinting is the photo-crosslinking speed. Without fast photo-crosslinking speed, GelMA cannot form structures with desired resolution during 3D bioprinting. Key to solve this challenge is to increase the speed of GelMA bio-ink photo-crosslinking reactions. It is shown that instead of methacrylation of gelatin, its modification with the acrylate functional group can demonstrate a higher photo-crosslinking rate. We demonstrated that the modification efficiency increased to 90% with 4:1 reagent ratio, compared to 67% for the degree of GelMA modification. The synthesized GelAc was then examined with human umbilical vein endothelial cells, which showed tubular network formation after 5 days culture, demonstrating good biocompatibility of GelAc hydrogel.

测试了实验室制造的3D生物生产者的功能和协议。由于其内在特征（例如生物相容性和可调的3D体系结构），基于纤维素的材料在构建细胞外基质脚手架脚手架上具有巨大的生物医学应用潜力。本文中，纳米纤维素是从木基纤维素处理的，以产生长度为100nm和直径5nm的纳米纤维。使用3D BioProinter，成功打印了纳米纤维素支架。用印刷的纳米纤维素支架培养成纤维细胞3T3细胞，细胞表现出良好的粘附和增殖行为。乙醇甲基丙烯酸酯（Gelma）已被广泛用作3D生物涂纸的支架材料。但是，使用Gelma进行3D生物打印的障碍之一是光合链接速度。如果没有快速的照片跨链接速度，Gelma将无法在3D生物打印过程中形成具有所需分辨率的结构。解决这一挑战的关键是提高Gelma Bio-Ink光合链接反应的速度。结果表明，与明胶的甲基丙烯酸化相反，其用丙烯酸酯官能团的修饰可以证明较高的光合链接速率。我们证明，以4：1的试剂比，修饰效率提高到90％，而Gelma修饰程度为67％。然后用人脐静脉内皮细胞检查合成的凝胶胶，该细胞在5天培养后显示了管状网络形成，表明凝胶状水凝胶的生物相容性良好。

项目成果

Minami Masumoto, Tatsuya Osaki, Tatsuto Kageyama, Binbin Zhang, Isao Sakane, Shintaro Takahashi, Shinichi Takimoto & Junji Fukuda

增本南、大崎达也、影山辰人、张彬彬、坂根功、高桥慎太郎、泷本真一

• 发表时间: 2019
• 作者: M. Julien;P. Hohener;R. J. Robins;J. Parinet and G. S. Remaud;Cell Morphology Monitoring during Culture for Daily Cell Quality Control
• 通讯作者: Cell Morphology Monitoring during Culture for Daily Cell Quality Control