Multicellular organization by a non-diffusible signal: Mathematical and experimental analysis of morphogenetic cell movements in myxobacteria

非扩散信号的多细胞组织：粘细菌形态发生细胞运动的数学和实验分析

• 批准号: 5451795
• 负责人: Professor Dr. Andreas Deutsch
• 金额: --
• 依托单位: Zentrum für Informationsdienste und Hochleistungsrechnen (ZIH)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5451795?language=en
• 关键词: Multicellular; organization; non; diffusible; signal

One of the essential problems in developmental biology is how spatial patterns of differentiated cells (e.g. tissues) can arise from an initially uniform mass of identical cells. Here, a unique group of biologists, mathematicians and physicists addresses this question using multicellular development in Myxococcus xanthus as a model system. The working group will apply a highly interdisciplinary approach combining biological experiments with quantitative mathematical modeling and simulation. In response to starvation, cells of the gliding bacterium M. xanthus initiate a multicellular developmental program that leads to streaming and aggregation patterns and culminates in the formation of spore-filled fruiting bodies. The non-diffusible C-signal plays a key role in inducing and choreographing the aggregation and sporulation processes. The main objective of this proposal is the construction and analysis of a quantitative mathematical model for the development of a three-dimensional fruiting body starting from individual cells. The mathematical model will allow us to investigate, whether the cell surface-associated C-signal is sufficient for explaining the multicellular organization of myxobacteria or if additional signaling mechanisms are required (e.g. a diffusive signaling mechanism, which plays a key role in the multicellular organization of the slime mould Dictyostelium discoideum). Model predictions will be validated in experiments with developmental mutants in which C-signaling is deficient. This project promises new insights into pattern formation mechanisms in biological systems and a better understanding of how non-diffusible morphogens may induce and organize morphogenetic cell movements.

发育生物学中的一个基本问题是分化细胞（例如组织）的空间模式如何从最初均匀的相同细胞群中产生。在这里，一组独特的生物学家，数学家和物理学家使用粘球菌作为模型系统的多细胞发育来解决这个问题。该工作组将采用高度跨学科的方法，将生物实验与定量数学建模和模拟相结合。滑行细菌M.苍耳启动多细胞发育程序，导致流和聚集模式，并最终形成充满孢子的子实体。不可扩散的C-信号在诱导和编排聚集和孢子形成过程中起着关键作用。该提案的主要目标是构建和分析从单个细胞开始的三维子实体发展的定量数学模型。数学模型将使我们能够调查，细胞表面相关的C-信号是否足以解释粘细菌的多细胞组织，或者是否需要额外的信号传导机制（例如，扩散信号传导机制，这在黏菌Dictyosteelium discoideum的多细胞组织中起着关键作用）。模型预测将在C-信号传导缺陷的发育突变体的实验中得到验证。该项目有望对生物系统中的模式形成机制产生新的见解，并更好地了解非扩散性形态发生素如何诱导和组织形态发生细胞运动。