Molecular Phylogeny of the Arthropoda and the "Ecdysozoa" Hypothesis

节肢动物的分子系统发育和“蜕皮动物”假说

• 批准号: 5453289
• 负责人: Professor Dr. Thorsten Burmester
• 金额: --
• 依托单位: Arbeitsbereich Molekulare Tierphysiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5453289?language=en
• 关键词: Molecular; Phylogeny; Arthropoda; Ecdysozoa; Hypothesis

Our project aims at the elucidation of the relationships among arthropods and their phylogenetic position in the Metazoa. Within the framework of the priority project 1174, we have sequenced ~28,000 ESTs (expressed sequence tags) from five arthropod species. Orthologous genes were extracted and a large scale phylogenomic approach (117 species, 129 genes [37,476 positions]) of arthropod relationships was conducted in cooperation with other groups of the priority project. A 454 pyrosequencing approach of scorpion ESTs (~428,000 reads; ~100 Mb) showed that this method is suitable for phylogenomic research. Based on single genes, we inferred that sea spiders (Pycnogonida) are the sister-group of Euchelicerata and found an unexpectedly close relationship of Remipedia and Hexapoda. Nevertheless, essential questions of arthropod phylogeny are still unresolved (e.g., position of Myriapoda). We will continue with the elucidation of arthropod phylogeny by mutigene analyses. ESTs from two myriapod and two chelicerate species will be generated by 454 pyrosequencing. When normalisation of cDNA shows an increase of single genes with sufficient quality, we will perform 454 runs of half plates per species and add ESTs from a tardigrade and a kinorhynchan to increase data coverage in Ecdysozoa. Based on concatenated multigene alignments, we will study arthropod and ecdysozoan phylogeny in cooperation with other groups. We will specifically focus on the position of the Myriapoda (Mandibulata vs. Myriochelata hypotheses) and the relationship among chelicerate and myriapod classes, considering effects like outgroup selection, variable evolution rates and base compositions. Molecular clock methods will be used to estimate divergence times between selected arthropods. In addition, hemocyanin and other selected genes will be used to infer the relationships among certain taxa.

本项目旨在阐明节肢动物之间的关系及其在后生动物中的系统发育位置。在优先项目1174的框架内，我们已经测序了来自5个节肢动物物种的约28000个est（表达序列标签）。提取同源基因，并与重点项目其他小组合作开展节肢动物亲缘关系的大规模系统基因组分析（117种，129个基因[37,476个位置]）。对蝎子ESTs的454焦磷酸测序方法（~428,000 reads; ~100 Mb）表明，该方法适用于系统基因组学研究。基于单基因分析，我们推测海蜘蛛（Pycnogonida）是Euchelicerata的姊妹类群，并意外地发现了Remipedia和Hexapoda的亲缘关系。然而，节肢动物系统发育的基本问题仍未解决（如多足纲的位置）。我们将继续通过多基因分析来阐明节肢动物的系统发育。通过454焦磷酸测序，将得到两种多足动物和两种螯合动物的ESTs。当cDNA归一化显示单个基因增加且质量足够时，我们将对每个物种进行454次半板测试，并添加来自缓步动物和kinorhynchan的est，以增加Ecdysozoa的数据覆盖率。基于串联的多基因比对，我们将与其他群体合作研究节肢动物和外生动物的系统发育。我们将特别关注多足类的位置（下颌与Myriochelata假说），以及螯合类和多足类之间的关系，考虑到外群选择、可变进化率和碱基组成等影响。分子钟方法将用于估计选定节肢动物之间的分化时间。此外，血青素和其他选择的基因将被用来推断某些分类群之间的关系。

项目成果

Dating the arthropod tree based on large-scale transcriptome data.

• DOI: 10.1016/j.ympev.2011.09.003
• 发表时间: 2011-12
• 期刊: Molecular phylogenetics and evolution
• 影响因子: 4.1
• 作者: P. Rehm;Janus Borner;K. Meusemann;B. V. von Reumont;Sabrina Simon;H. Hadrys;B. Misof;T. Burmester

EST sequencing of Onychophora and phylogenomic analysis of Metazoa.

• DOI: 10.1016/j.ympev.2007.09.002
• 发表时间: 2007-12
• 期刊: Molecular phylogenetics and evolution
• 影响因子: 4.1
• 作者: F. Roeding;Silke Hagner-Holler;H. Ruhberg;I. Ebersberger;A. von Haeseler;M. Kube;R. Reinhardt;T. Bur

Remipedia and the Evolution of Hexapods

Remipedia 和六足动物的进化

• DOI: 10.1002/9780470015902.a0022862
• 发表时间: 2010
• 作者: von Reumont;Burmester
• 通讯作者: Burmester