黄色猩々蝿のＴＡＬＥモジュレーションを利用した転写因子群の二機能性に関する研究

在2019财年，这位研究员的角色是开发实验模型，以测试使用融合到激活器域或抑制器域的转录激活器样(TALE)蛋白来测试合成调控电路(Crocker等人，2016)。通过增加或减少增强剂中的故事结合位点的数量来操纵TFS的贡献量。采用了与TALL融合到BCD的相同方法；通过调整TALL结合位点的数量，将观察BCD活性逐步增加的影响，从而测试它是否以及在什么时候从抑制者转换为激活者。优化了产生和组装组成转基因构建物的必要片段的实验条件。然后，它们可以被用来创造转基因果蝇，供以后应用。综上所述，这些步骤的成功为后续实验提供了强大的技术基础和良好的前景。

In FY2019, the fellow’s role was to develop the experimental model to test synthetic regulatory circuits using transcription activator-like (TALE) proteins fused to activator or repressor domains (Crocker et al., 2016). The amount of the TFs contribution is manipulated by adding or subtracting the number of TALE-binding sites in the enhancer. The same approach with TALE fused to Bcd was applied; by adjusting the number of TALE binding sites, the impact of the stepwise increase in Bcd activity will be observed, so testing whether and at what point it switches from repressor to activator. Experimental conditions for generating and assembling essential fragments that compose of transgenic constructs were optimized. They can then be used to create transgenic flies for applications later on. Altogether, the success of these steps provides a strong technical basis and shows great promises for downstream experiments.