黄色猩々蝿のＴＡＬＥモジュレーションを利用した転写因子群の二機能性に関する研究

在2019财年，该研究员的职责是开发实验模型，以测试使用与激活子或阻遏物结构域融合的转录激活子样（TALE）蛋白的合成调控回路（Crocker et al.，2016）。TF贡献的量通过添加或减去增强子中的TALE结合位点的数目来操纵。应用与TALE融合至Bcd的相同方法;通过调整TALE结合位点的数量，将观察Bcd活性逐步增加的影响，从而测试它是否以及在什么时候从阻遏物转换为激活物。优化了用于产生和组装组成转基因构建体的必需片段的实验条件。总而言之，这些步骤的成功为下游实验提供了坚实的技术基础，并显示出巨大的前景。

In FY2019, the fellow’s role was to develop the experimental model to test synthetic regulatory circuits using transcription activator-like (TALE) proteins fused to activator or repressor domains (Crocker et al., 2016). The amount of the TFs contribution is manipulated by adding or subtracting the number of TALE-binding sites in the enhancer. The same approach with TALE fused to Bcd was applied; by adjusting the number of TALE binding sites, the impact of the stepwise increase in Bcd activity will be observed, so testing whether and at what point it switches from repressor to activator. Experimental conditions for generating and assembling essential fragments that compose of transgenic constructs were optimized. They can then be used to create transgenic flies for applications later on. Altogether, the success of these steps provides a strong technical basis and shows great promises for downstream experiments.