黄色猩々蝿のＴＡＬＥモジュレーションを利用した転写因子群の二機能性に関する研究

在2019财年，该研究员的角色是开发实验模型，使用转录激活因子样（TALE）蛋白融合到激活因子或抑制因子结构域来测试合成调控电路（Crocker等人，2016）。通过增加或减少增强子中tale结合位点的数量来控制TFs的贡献量。采用同样的方法，将TALE与Bcd融合；通过调整TALE结合位点的数量，可以观察到Bcd活性逐步增加的影响，因此可以测试它是否以及在什么时候从抑制因子转变为激活因子。优化了构建转基因构建体所需片段的生成和组装实验条件。然后，它们可以用来创造转基因果蝇，以供以后的应用。总之，这些步骤的成功为下游实验提供了强大的技术基础，并显示出巨大的希望。

In FY2019, the fellow’s role was to develop the experimental model to test synthetic regulatory circuits using transcription activator-like (TALE) proteins fused to activator or repressor domains (Crocker et al., 2016). The amount of the TFs contribution is manipulated by adding or subtracting the number of TALE-binding sites in the enhancer. The same approach with TALE fused to Bcd was applied; by adjusting the number of TALE binding sites, the impact of the stepwise increase in Bcd activity will be observed, so testing whether and at what point it switches from repressor to activator. Experimental conditions for generating and assembling essential fragments that compose of transgenic constructs were optimized. They can then be used to create transgenic flies for applications later on. Altogether, the success of these steps provides a strong technical basis and shows great promises for downstream experiments.