Cell-free analysis of phagosome biogenesis in macrophages

巨噬细胞吞噬体生物发生的无细胞分析

• 批准号: 54760881
• 负责人: Professor Dr. Albert Haas
• 金额: --
• 依托单位: Institut für Zellbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/54760881?language=en
• 关键词: Cell; free; analysis; phagosome; biogenesis

Microorganisms that have entered our body are quickly ingested by phagocytic cells of the immune system (in particular, macrophages and neutrophils). The microbe is wrapped into a portion of the phagocyte’s plasma membrane, ingested by the host cell, and a new organelle (phagosome) is created. This compartment is similar to endosomes and ‘matures’ into a phagolysosome in which most microorganisms are killed and degraded. Little is known about how precisely maturation proceeds and which factors are required in the different maturation steps. This study will define stage-specific factors for the fusion of endosomes/lysosomes with phagosomes in a cell-free system and will analyse the dynamic changes in fusion competences of phagosomes that contain intracellular pathogens. The results foreseen will help us to define the maturation of phagosomes at the molecular level and set the stage for understanding how intracellular pathogens manipulate vesicular trafficking in macrophages.

进入我们体内的微生物很快就会被免疫系统的吞噬细胞（特别是巨噬细胞和中性粒细胞）吞噬。微生物被包裹在吞噬细胞质膜的一部分中，被宿主细胞摄入，并产生一个新的细胞器（吞噬体）。这个区室类似于内体，并“成熟”成吞噬溶酶体，其中大多数微生物被杀死和降解。关于成熟过程的精确程度以及不同成熟步骤中需要哪些因素，人们知之甚少。本研究将定义无细胞系统中内体/溶酶体与吞噬体融合的阶段特异性因素，并将分析含有细胞内病原体的吞噬体融合能力的动态变化。预见的结果将帮助我们在分子水平上定义吞噬体的成熟，并为理解细胞内病原体如何操纵巨噬细胞中的囊泡运输奠定基础。