权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

初期生育におけるヘテロシス（雑種強勢）の発現機構の解明と育種利用

阐明早期生长期间杂种优势（杂种优势）的表达机制及其在育种中的应用

基本信息

批准号：
22K05569
负责人：
北崎一義
金额：
$ 2.66万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-01 至 2025-03-31
项目状态：
未结题

项目摘要

６組の組み合わせの親系統とそのF1系統のテンサイを供試し、初期生育のヘテロシスの発現機構に関する研究を行った。まず、栽培期間中の成長の推移を詳細に調査するため、葉面積を非破壊で測定できる方法を開発した。供試材料を水耕栽培し、葉面積を個体ごとに毎日調査した。系統間および組み合わせ間で比較したところ、ヘテロシスの程度の違いは発芽後１５日頃にはほぼ決まっていることや、ほとんどの組み合わせでF1は親よりも相対成長速度が速いことが明らかとなった。同材料を圃場で栽培し、中期生育、収穫期生育および収量を調査した。系統ごとに乾物重量を求め栽培条件間で比較したところ、水耕栽培による初期生育、中期生育および収穫期生育間で有意な高い相関が見つかった。さらに組み合わせによるヘテロシスの程度にも高い相関が認められた。一方、収量との相関はこれらと比べて低かった。本試験は、収量調査区は慣行法で、それ以外は隣接個体間で干渉しないように株間を広くした。このことから、慣行法による収量には密植適性や収穫部位への乾物分配などの成長以外の要因が寄与していることが示唆された。屋内水耕栽培装置を増設し、供試系統を追加した合計１９の組み合わせを用いて、ヘテロシスの程度を決定する遺伝要因について研究を行った。一部の供試系統は新たに全ゲノムシーケンスを行い、塩基多型解析によって供試系統間の遺伝距離を算出した。組み合わせによる初期生育のヘテロシスの程度の差異と遺伝距離を比較したところ、弱い相関が認められた。先行研究でテンサイでは両親の近縁係数とヘテロシスの程度に強い負の相関が報告されているが、完全には一致しなかった。一方、この相関関係から著しく逸脱した組み合わせも見つかったことから、ヘテロシスに寄与率が高いゲノム領域が存在することが示唆された。

6 groups of parents and F1 systems were tested, and the development mechanism of early fertility was studied. A detailed investigation on the growth and development of leaves during cultivation was carried out and a method for non-destructive measurement of leaf area was developed. The test materials were hydroponic cultivated, and their leaf areas were investigated daily. 15 days after the system was developed, the system was divided into two groups: the first group and the second group. The same material was cultivated in the nursery, the middle stage of growth, the harvest stage of growth and the amount of growth were investigated. Comparison of dry matter weight between cultivation conditions, hydroponic cultivation, early growth, middle growth, harvest growth, intentional high correlation The first is to set up a joint venture. A side, a side. In this experiment, the study area was divided into two groups: one group was divided into two groups, the other group was divided into two groups, the other group was divided into two groups. This is the first time that a plant has been planted in a dry area, and the second time that a plant has been planted in a dry area. A total of 19 groups were added to the indoor hydroponic cultivation equipment, and the test system was used to determine the main factors for the study. A part of the test system is composed of three parts: the first part is composed of four parts: the first part is composed of three parts: the first part is composed of four parts: the first part is composed of four parts: the second part is composed of four parts: the first part is composed of four parts: the second part is composed of four parts: the first part is composed of four parts: the first part is composed of four parts: the second part is composed of four parts: the third part is composed of four parts: the fourth part is The difference between the degree of fertility and the distance between the two groups is relatively small. In the previous study, the correlation coefficient of the parent and the degree of the parent were strongly negative and completely consistent. A party, the correlation between the two groups, the relationship between the two groups, the relationship between the two groups, the relationship