統合失調症と気分障害における作業記憶ネットワーク障害のニューロンメカニズム

精神分裂症和情绪障碍工作记忆网络损伤的神经机制

• 批准号: 22K07575
• 负责人: 川端 梨加
• 金额: $ 2.5万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K07575/
• 关键词: 死後脳; GABAニューロン; real-time PCR; in situ hybridization

本年度は、統合失調症患者、双極性障害患者、うつ病患者、並びに精神神経疾患の罹患歴のない健常対照者の死後脳より背外側前頭前野の灰白質組織を白質の混入を避けて注意深く切り出し、Trizol液に撹拌しRNA抽出キットを用いて遺伝子発現解析に用いるサンプルを準備した。性別が同じで、年齢、死後経過時間、脳の状態を反映する脳組織pHなどが近い、各患者および対照者のそれぞれ1名の計4名を1組とする40組（各グループ40名）よりRNAサンプルを準備した。死後脳提供者の性別は、各グループとも男性22名、女性18名（X2(3) = 0.00; p = 1.00）、平均年齢(SD)は、統合失調症患者群が47.4(12.2)歳、双極性障害患者群が45.8(12,4)歳、うつ病患者群が45.5(11.7)歳、精神神経疾患の罹患歴のない健常対照者（UC）が46.8(12.8)歳で、グループ間での有意差は無かった（F3,156 = 0.20; p = 0.90）。脳組織の死後経過時間の平均(SD)は、SZが19.9(7.4)時間、BPが20.8(7.0)時間、MDが19.2(5.8)時間、UCが19.6(5.6)時間で、同じく有意差は無かった（F3,156 = 0.46; p = 0.71）。脳の状態を反映する脳組織pH(SD)は、SZが6.5(0.3)、BPが6.6(0.3)、MDが6.5(0.2)、UCが6.7(0.2)で、同じく有意差は無かった（F3,156 = 1.83; p = 0.15）。大脳皮質より抽出されたRNAサンプルのRNA integrity number (RNAの品質を評価する指標：RIN) (SD)は、SZが8.0(0.6)、BPが7.9(0.7)、MDが8.0(0.6)、UCが8.1(0.6)で、同じく有意差は無かった（F3,156 = 0.77; p = 0. 51）。

This year, patients with integration disorders, bipolar disorders, patients with neuropathy, and patients with neuropsychiatric disorders are often diagnosed with postmortem, gray and white matter in the front of the dorsolateral front of the head and front of the field. The tissue is mixed with white matter, and the tissue is carefully cut and extracted, and the Trizol solution is mixed and the RNA is extracted, and the RNA is extracted and analyzed using the いるサンプルを preparation. Gender, age, time since death, and patient status reflect the patient's tissue pH, closeness, and photos of each patient. There are 1 person in total, 4 people in total, 1 group, 40 people in each group (40 people in each group), and RNA サンプルを is ready. As for the gender of the medical providers, 22 were male and 18 were female (X2(3) = 0.00; p = 1.00), average age (SD), integration disorder patients: 47.4 (12.2) years, bipolar disorder patients: 45.8 (12,4) years, bipolar disorder patients: 45 .5 (11.7) years old, those who suffer from mental and neurological diseases, those who often take care of their health (UC), 46.8 (12.8) years old, those who suffer from mental and neurological diseases, and those who suffer from mental and neurological diseases, there is no intentional difference (F3,156 = 0.20; p = 0.90). The average post-mortem time of the tissue (SD), SZ 19.9 (7.4) time, BP 20.8 (7.0) time, MD 19.2 (5.8) time, UC 19.6 (5.6) time, same time and no intentional difference (F3, 156 = 0.46; p = 0.71). The state of the fish reflects the pH (SD) of the tissue, SZ 6.5 (0.3), BP 6.6 (0.3), MD 6.5 (0.2), UC 6.7 (0.2), and the same but not the same (F3,156 = 1.83; p = 0.15). The RNA integrity number (RNA quality evaluation index: RIN) (SD)は, SZが8.0(0.6), BPが7.9(0.7), MDが8.0(0.6), UCが8.1(0.6)で, sameじくmeaningful differenceは无かった (F3,156 = 0.77; p = 0. 51).

项目成果

ピッツバーグ大学(米国)

匹兹堡大学（美国）