Substrate-translocase interactions during two-partner secretion in Gram-negative bacteria
革兰氏阴性菌两伙伴分泌过程中底物-转位酶的相互作用
基本信息
- 批准号:61723904
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Units
- 财政年份:2008
- 资助国家:德国
- 起止时间:2007-12-31 至 2014-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The two-partner secretion (TPS-) pathway found in pathogenic Gram-negative bacteria is dedicated to the secretion of virulence factors (termed TpsA-proteins). TpsA-proteins are translocated via the Sec translocon to the periplasm, from where they are exported through a β-barrel pore that is formed by the cognate TpsB protein in the outer membrane. A related transport system is that of autotransporters, in which the secreted protein is C-terminally fused to a β-barrel domain that mediates translocation across the outer membrane. Recently we have developed a novel in vitro system for the study of translocation of a TpsA protein across its TpsB-pore. We now want to use this system to identify periplasmic proteins involved in chaperoning and targeting a TpsA protein to the TpsB-pore. We will use site-specific photo-crosslinking in combination with the generation of translocation intermediates of a TpsA-protein stalled in the TpsB-pore, to determine the interacting sites between TpsA and TpsB, the actual translocation path across TpsB, and the orientation by which TpsA is threaded into TpsB. By use of inactivating mutations of the TpsA-protein we want to examine if its post-translocational folding provides the driving force and unidirectionality of transport. We will further exploit the cell-free system to study the related autotransporter pathway.
在致病性革兰氏阴性菌中发现的双伴侣分泌(TPS-)途径致力于分泌毒力因子(称为TpsA-蛋白)。TpsA蛋白通过Sec易位子易位到周质,从周质通过由外膜中的同源TpsB蛋白形成的β桶孔输出。相关的转运系统是自转运蛋白的转运系统,其中分泌的蛋白质在C末端融合至介导跨外膜易位的β-桶结构域。最近,我们开发了一种新的体外系统,用于研究TpsA蛋白穿过其TpsB孔的易位。我们现在想使用这个系统来鉴定参与伴侣作用和将TpsA蛋白靶向TpsB孔的周质蛋白。我们将使用位点特异性光交联结合产生的TpsA-蛋白质的易位中间体停滞在TpsB-孔,以确定TpsA和TpsB之间的相互作用位点,跨TpsB的实际易位路径,以及TpsA被线程到TpsB的方向。通过使用TpsA蛋白的失活突变,我们想要检查其易位后折叠是否提供了运输的驱动力和单向性。我们将进一步利用无细胞系统研究相关的自转运途径。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Mapping Precursor-binding Site on TatC Subunit of Twin Arginine-specific Protein Translocase by Site-specific Photo Cross-linking*
- DOI:10.1074/jbc.m112.343798
- 发表时间:2012-02
- 期刊:
- 影响因子:0
- 作者:Stefan Zoufaly;J. Fröbel;Patrick H Rose;T. Flecken;C. Maurer;Michael Moser;Matthias Müller
- 通讯作者:Stefan Zoufaly;J. Fröbel;Patrick H Rose;T. Flecken;C. Maurer;Michael Moser;Matthias Müller
Versatile in vitro system to study translocation and functional integration of bacterial outer membrane proteins
- DOI:10.1038/ncomms6396
- 发表时间:2014-11
- 期刊:
- 影响因子:16.6
- 作者:Derrick Norell;A. Heuck;T. Tran‐Thi;H. Götzke;F. Jacob-Dubuisson;T. Clausen;D. Daley;V. Braun;Matthias Müller;Enguo Fan
- 通讯作者:Derrick Norell;A. Heuck;T. Tran‐Thi;H. Götzke;F. Jacob-Dubuisson;T. Clausen;D. Daley;V. Braun;Matthias Müller;Enguo Fan
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Professor Dr. Matthias Müller其他文献
Professor Dr. Matthias Müller的其他文献
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{{ truncateString('Professor Dr. Matthias Müller', 18)}}的其他基金
Regional networks and supraregional ambitions. Medieval Ecclesiastical Architecture in the Middle Rhine Valley (1250 to 1450)
区域网络和跨区域的野心。
- 批准号:
234541719 - 财政年份:2013
- 资助金额:
-- - 项目类别:
Research Grants
Fremdbilder - Selbstbilder: Kulturtransfer als ästhetischer und politisch-religiöser Diskurs in höfischen Bildkonzepten des späten Mittelalters und der Frühen Neuzeit im Alten Reich
外国图像-自我图像:作为中世纪晚期和近代早期古王国宫廷图像概念中的审美和政治宗教话语的文化转移
- 批准号:
23896076 - 财政年份:2006
- 资助金额:
-- - 项目类别:
Research Grants
Die mittelalterlichen Paramente aus der Hansestadt Stralsund. Kunsthistorische und textilkundliche Auswertung
来自汉萨城市施特拉尔松德的中世纪装饰。
- 批准号:
5405182 - 财政年份:2003
- 资助金额:
-- - 项目类别:
Research Grants
In vitro analysis of the bacterial twin-arginine translocation pathway
细菌双精氨酸易位途径的体外分析
- 批准号:
5230234 - 财政年份:2000
- 资助金额:
-- - 项目类别:
Research Grants
Garden and music theatre at the Dresden court in the 17th and 18th century: Medial and functional interrelations in the service of stately metaphor and princely representation
17 世纪和 18 世纪德累斯顿宫廷的花园和音乐剧院:为庄严的隐喻和王子的表现服务的中间和功能的相互关系
- 批准号:
460857984 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Grants
Order and aura of courtly things: the Dresden Kunstkammer of the 16th and 17th centuries as a place of political interaction, dynastic memoria and princely knowledge practice
宫廷事物的秩序和光环:16 世纪和 17 世纪的德累斯顿艺术馆,作为政治互动、王朝记忆和王子知识实践的场所
- 批准号:
440653566 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Grants
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