Identification of keystone prokaryotic food web members and links in belowground carbon turnover - SP MicLink

关键原核食物网成员的鉴定和地下碳周转的链接 - SP MicLink

• 批准号: 62031580
• 负责人: Professor Dr. Tillmann Lüders
• 金额: --
• 依托单位: Lehrstuhl für Ökologische Mikrobiologie (ÖMIK)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/62031580?language=en
• 关键词: Identification; keystone; prokaryotic; food; web

The MicLink project within FOR aims to elaborate a precise understanding of trophic connectivities and carbon flow amongst bacterial members of a belowground food web. In the first phase of funding, we have generated a unique matrix of bacterial community structure and distribution at our joint experimental field site, partly based on pyrotag sequencing, and identified several primary plant-dependent bacterial food web constituents by rRNA-SIP. In the next phase, we want to address an emerging hypothesis that while protozoan predation may well be a relevant control of Bacteria in plant-associated soil, intra-bacterial predation e.g. by Myxobacteria may be of overlooked importance especially in bulk soil. Furthermore, we have preliminarily identified specific subsets of rhizosphere Bacteria that seem to be selectively mobilised to deeper zones upon groundwater recharge events. This could represent an organismic and trophic connectivity between top soil and deeper mineral soils that is still poorly understood. Now, we plan to quantify microbial mobilisation rates and fluxes using amended 13C-labelled biomass and single-cell recovery in seepage water using NanoSIMS. Moreover, we will continue our field and laboratory contribution to joint experiments, focussing now on herbivore vs. detrital food chains, and on quantitative fluxes in selected food web connectivities. The provided qualitative and quantitative data on keystone microbial food web connectivities will be an essential prerequisite for a realistic modelling of carbon flow in belowground food webs.

内部的Miclink项目旨在详细了解地下食物网的细菌成员之间对营养连接性和碳流动的精确理解。在资金的第一阶段，我们在联合实验场现场产生了一个独特的细菌群落结构和分布的矩阵，部分基于Pyrotag测序，并通过rRNA-SIP确定了几种主要的植物依赖性细菌Web成分。在下一阶段，我们要解决一个新出现的假设，即尽管原生动物捕食很可能是对植物相关土壤中细菌，但细菌内捕食的相关控制，例如通过粘菌病，尤其是在散装土壤中，粘菌病很重要。此外，我们最初确定了根际细菌的特定亚群，这些细菌似乎被选择地动员到地下水补给事件上的较深区域。这可以代表顶部土壤和更深的矿物土壤之间的有机和营养的连通性，但仍知之甚少。现在，我们计划使用纳米菌素在渗水中使用经修订的13C标记的生物质和单细胞回收来量化微生物动员速率和通量。此外，我们将继续对联合实验的野外和实验室贡献，现在专注于草食动物与碎屑食品链，以及选定食品网络连接性的定量通量。提供的有关Keystone微生物食品网络连接性的定性和定量数据将是对地下食物网中碳流进行现实建模的必要先决条件。