Identification of keystone prokaryotic food web members and links in belowground carbon turnover - SP MicLink

关键原核食物网成员的鉴定和地下碳周转的链接 - SP MicLink

• 批准号: 62031580
• 负责人: Professor Dr. Tillmann Lüders
• 金额: --
• 依托单位: Lehrstuhl für Ökologische Mikrobiologie (ÖMIK)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/62031580?language=en
• 关键词: Identification; keystone; prokaryotic; food; web

The MicLink project within FOR aims to elaborate a precise understanding of trophic connectivities and carbon flow amongst bacterial members of a belowground food web. In the first phase of funding, we have generated a unique matrix of bacterial community structure and distribution at our joint experimental field site, partly based on pyrotag sequencing, and identified several primary plant-dependent bacterial food web constituents by rRNA-SIP. In the next phase, we want to address an emerging hypothesis that while protozoan predation may well be a relevant control of Bacteria in plant-associated soil, intra-bacterial predation e.g. by Myxobacteria may be of overlooked importance especially in bulk soil. Furthermore, we have preliminarily identified specific subsets of rhizosphere Bacteria that seem to be selectively mobilised to deeper zones upon groundwater recharge events. This could represent an organismic and trophic connectivity between top soil and deeper mineral soils that is still poorly understood. Now, we plan to quantify microbial mobilisation rates and fluxes using amended 13C-labelled biomass and single-cell recovery in seepage water using NanoSIMS. Moreover, we will continue our field and laboratory contribution to joint experiments, focussing now on herbivore vs. detrital food chains, and on quantitative fluxes in selected food web connectivities. The provided qualitative and quantitative data on keystone microbial food web connectivities will be an essential prerequisite for a realistic modelling of carbon flow in belowground food webs.

For内的MicLink项目旨在详细阐述对地下食物网中细菌成员之间的营养连接和碳流的精确理解。在第一阶段的资助中，我们在我们的联合实验场产生了一个独特的细菌群落结构和分布矩阵，部分基于焦油标签测序，并通过rRNA-SIP鉴定了几个主要的植物依赖型细菌食物网成分。在下一阶段，我们希望解决一个新的假设，即虽然原生动物的捕食很可能是对植物相关土壤中细菌的相关控制，但细菌内的捕食，例如粘杆菌的捕食，可能被忽视，特别是在散装土壤中。此外，我们已经初步确定了根际细菌的特定亚群，这些细菌似乎在地下水补给事件时选择性地被动员到更深的区域。这可能代表了表层土壤和深层矿物土壤之间的有机和营养联系，但人们对此仍知之甚少。现在，我们计划使用修正的13C标记生物量来量化微生物的动员速率和通量，并使用NanoSIMS在渗漏水中进行单细胞回收。此外，我们将继续在现场和实验室为联合实验做出贡献，现在重点关注食草动物与碎屑食物链，以及选定食物网络连接性中的定量通量。所提供的关于Keystone微生物食物网连接性的定性和定量数据将是对地下食物网中的碳流进行现实建模的基本前提。