BSS and beyond - functional markers to unravel the ecology of anaerobic hydrocarbon degraders in the environment

BSS 及其他 - 揭示环境中厌氧碳氢化合物降解剂生态的功能标记

• 批准号: 71779386
• 负责人: Professor Dr. Tillmann Lüders
• 金额: --
• 依托单位: Lehrstuhl für Ökologische Mikrobiologie (ÖMIK)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/71779386?language=en
• 关键词: BSS; beyond; functional; markers; unravel

Until recently, the understanding of the identity and diversity of microbial key players involved in anaerobic hydrocarbon degradation in environmental systems remained almost exclusively based on a relatively small number of laboratory cultures. To address this problem, we have recently established a functional marker concept for fumarate-adding catabolic genes involved in the anaerobic degradation of many hydrocarbons. We now want to implement this in an innovative pyrotag sequencing approach to rapidly and comprehensively assess anaerobic hydrocarbon degrader diversities in environmental samples. This approach is employed to investigate central links between structure and functioning of anaerobic hydrocarbon degrader communities. Thus we test our hypothesis that more diverse degrader communities also harbour a greater level of functional redundancy, and thus are more robust to hydrogeochemical habitat dynamics. Moreover, we will address central ecological mechanisms that may actually cause the establishment of more or less diverse degrader communities in different contaminated ecosystems. Thus we will contribute to primary objectives of the proposed DFG Priority Programme, the identification of the true diversity of relevant microbial key-players in anaerobic hydrocarbon degradation in natural systems, and the elucidation of the ecology of contaminant plumes as a habitat for hydrocarbon degrading microbes.

直到最近，对环境系统中参与厌氧烃降解的微生物关键角色的身份和多样性的理解几乎完全基于相对较少的实验室培养。为了解决这个问题，我们最近建立了一个功能标记概念，用于添加富马酸的分解代谢基因，这些基因涉及许多碳氢化合物的厌氧降解。我们现在想在一种创新的高温标签测序方法中实现这一目标，以快速全面地评估环境样品中厌氧烃降解物的多样性。这种方法被用来研究厌氧烃降解菌群落结构和功能之间的中心联系。因此，我们验证了我们的假设，即更多样化的降解生物群落也具有更高水平的功能冗余，因此对水文地球化学栖息地动态更强。此外，我们将讨论可能导致在不同污染生态系统中建立或多或少不同的降解群落的核心生态机制。因此，我们将为拟议的DFG优先计划的主要目标做出贡献，确定自然系统中厌氧烃降解中相关微生物关键角色的真正多样性，并阐明污染物羽流作为烃降解微生物栖息地的生态学。