The role of the plasma membrane-associated E3 ubiquitin ligase SAUL1 in suppression of premature senescence and reduced growth in plants

质膜相关 E3 泛素连接酶 SAUL1 在抑制植物过早衰老和生长减缓中的作用

• 批准号: 67172109
• 负责人: Professor Dr. Stefan Hoth
• 金额: --
• 依托单位: Abteilung Molekulare Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/67172109?language=en
• 关键词: role; plasma; membrane; associated; E3

Like in many organisms, plant senescence commences age-dependently and leads to cell death. This process becomes visible by chlorophyll degradation resulting in yellowing of leaves. Leaf senescence is genetically associated with aging and developmentally welldefined to guarantee recycling of resources from senescing leaves into young leaves or seeds to optimize growth and reproduction. We will use saul1 mutant plants, which we have established as low light-inducible, age-independent and highly reproducible model system for senescence and cell death, to decipher the underlying regulatory signaling network. Association of the E3 ubiquitin ligase SAUL1 and its closest homologue to the plasma membrane renders them extremely interesting regulators of key signaling components at the plasma membrane. We are aiming to find out the mechansim for SAUL1 plasma membrane association and to identify all interacting proteins as well as putative targets for ubiquitination through SAUL1. We will also use the saul1 model system to define similarities and differences between low light and salt or osmotic stress responses, respectively. Our research project will help to understand the molecular regulation of senescence and cell death that attribute for limited yield in agriculture.

与许多生物体一样，植物衰老的依赖性依赖性并导致细胞死亡。叶绿素降解可见，导致叶片泛黄。叶片衰老在遗传上与衰老相关，并在发育方面有很好的定义，以确保从衰老的叶子中回收资源，从叶片或种子中进行优化，以优化生长和繁殖。我们将使用SAUL1突变植物，我们已将其确定为低光诱导型，年龄独立且高度可重复的模型系统，以破译潜在的调节信号网络。 E3泛素连接酶SAUL1及其与质膜最接近的同源物的关联使它们在质膜上非常有趣的关键信号成分调节剂。我们的目标是找出SAUL1质膜关联的机甲，并确定所有相互作用的蛋白质以及通过SAUL1泛素化的推定靶标。我们还将使用SAUL1模型系统分别定义低光和渗透应力反应之间的相似性和差异。我们的研究项目将有助于理解衰老和细胞死亡的分子调节，这归因于农业产量有限。

项目成果

Early Senescence and Cell Death in Arabidopsis saul1 Mutants Involves the PAD4-Dependent Salicylic Acid Pathway1[W][OA]

• DOI: 10.1104/pp.112.196220
• 发表时间: 2012-06
• 期刊: Plant Physiology
• 影响因子: 7.4
• 作者: Katja Vogelmann;Gabriele Drechsel;Johannes Bergler;C. Subert;K. Philippar;J. Soll;Julia C. Engelmann;Timo Engelsdorf;L. Voll;S. Hoth