Genomics of host and habitat adaption in an entomopathogenic fungus

昆虫病原真菌宿主和栖息地适应的基因组学

• 批准号: 68585209
• 负责人: Professorin Dr. Annette Reineke
• 金额: --
• 依托单位: Institut für Phytomedizin
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/68585209?language=en
• 关键词: Genomics; host; habitat; adaption; entomopathogenic

Biopesticides based on entomopathogenic fungi are used worldwide for biological control of many insect pests. In a previous study (DST–DAAD collaboration project 2003–2006) we examined differentially expressed genes in the fungus Beauveria bassiana during the infection process of various insect cuticles and identified a subset of genes having a putative role in virulence and pathogenicity. Detailed molecular and functional characterization of these genes could be a promising approach to get hold of targets for improving the efficiency of B. bassiana based biopesticides. As a sequel to our previous work, gene expression patterns of the identified genes will be further characterized through quantitative Real-Time-PCR. In addition, sequence analysis of the respective full-length genes will be carried out. Recent studies (Bidochka et al. 2002) have further revealed that distinct genetic groups of B. bassiana are associated with different habitat types having obvious implications in the utility of this fungus as a biocontrol agent. The genetic basis of this habitat adaptation has not been identified yet. By applying the same B. bassiana isolate to temperate (German) and tropical (Indian) soils and monitoring any genetic changes during the course of colonization of the rhizophere, we aim to identify candidate genes relevant for the habitat adaptation process which is the underlying feature for the ubiquitous nature of this fungus.

基于昆虫病原真菌的生物农药在世界范围内用于许多害虫的生物防治。在之前的一项研究（DST-DAAD 合作项目 2003-2006）中，我们检查了白僵菌在各种昆虫角质层感染过程中差异表达的基因，并鉴定了在毒力和致病性中具有推定作用的基因子集。这些基因的详细分子和功能特征可能是一种有前途的方法，可以找到提高白僵菌生物农药效率的靶标。作为我们之前工作的后续工作，已识别基因的基因表达模式将通过定量实时 PCR 进一步表征。此外，还将进行各个全长基因的序列分析。最近的研究（Bidochka 等人，2002）进一步揭示白僵菌的不同遗传群体与不同的栖息地类型相关，这对该真菌作为生物防治剂的效用具有明显的影响。这种栖息地适应的遗传基础尚未确定。通过将相同的球孢白僵菌分离物应用于温带（德国）和热带（印度）土壤，并监测根际定殖过程中的任何遗传变化，我们的目标是识别与栖息地适应过程相关的候选基因，这是这种真菌普遍存在的基本特征。