Identification and characterization of NEDD8-modified proteins and deneddylases of the filamentous fungs Aspergillus nidulans

丝状真菌构巢曲霉 NEDD8 修饰蛋白和 Deneddylases 的鉴定和表征

• 批准号: 71503125
• 负责人: Professor Dr. Gerhard H. Braus
• 金额: --
• 依托单位: Abteilung für Molekulare Mikrobiologie und Genetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/71503125?language=en
• 关键词: Identification; characterization; NEDD8; modified; proteins

Neddylation is required for vegetative growth of the filamentous fungal model system Aspergillus nidulans, whereas deneddylation by the COP9 signalsome (CSN) is only essential for the sexual life cycle. In addition, CSN is required for light control and coordination of secondary metabolism. CSN interacts in a yeast-2-hybrid system to DenA/Den1, another deneddylase which is required for the asexual cycle of the fungus. We will explore the function of the interaction between CSN and DenA/1 and other putative interaction partners as cullins or the two fungal CandA/1 proteins. We will especiallyemphasize whether DenA/1 interacts to the entire CSN or only to subcomplexes. A tagged RubA/Nedd8 recruits in csnE/Csn5 mutants but not in wildtype distinct components of cullin complexes and several associated proteins. We will analyse whether there is a specific CSN control of developmental-specific fungal cullin complexes and their putative substrates. Finally we will explore whether there is a crosstalk between the Nedd8 and the SUMO conjugation (or deconjugation) pathway during fruit body formation of the sexual cycle of this fungus, because a deletion of the gene for SUMO results in a similar developmental phenotype as a deneddylase deficient mutant of A. nidulans.

Neddylation 是丝状真菌模型系统 Aspergillus nidulans 的营养生长所必需的，而 COP9 信号体 (CSN) 的 deneddylation 仅对有性生命周期至关重要。此外，CSN是光控制和次级代谢协调所必需的。 CSN 在酵母 2 杂交系统中与 DenA/Den1 相互作用，DenA/Den1 是真菌无性循环所需的另一种脱脂酶。我们将探索 CSN 和 DenA/1 以及其他假定的相互作用伙伴（如 cullins 或两种真菌 CandA/1 蛋白）之间相互作用的功能。我们将特别强调 DenA/1 是与整个 CSN 相互作用还是仅与子复合体相互作用。标记的 RubA/Nedd8 在 csnE/Csn5 突变体中招募，但不在野生型 cullin 复合物和几种相关蛋白的独特成分中招募。我们将分析是否存在对发育特异性真菌 cullin 复合物及其假定底物的特定 CSN 控制。最后，我们将探讨在这种真菌的性周期子实体形成过程中，Nedd8 和 SUMO 接合（或解接合）途径之间是否存在串扰，因为 SUMO 基因的缺失会导致与构巢曲霉的 deneddylase 缺陷突变体相似的发育表型。