Identification and characterization of NEDD8-modified proteins and deneddylases of the filamentous fungs Aspergillus nidulans

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Neddylation is required for vegetative growth of the filamentous fungal model system Aspergillus nidulans, whereas deneddylation by the COP9 signalsome (CSN) is only essential for the sexual life cycle. In addition, CSN is required for light control and coordination of secondary metabolism. CSN interacts in a yeast-2-hybrid system to DenA/Den1, another deneddylase which is required for the asexual cycle of the fungus. We will explore the function of the interaction between CSN and DenA/1 and other putative interaction partners as cullins or the two fungal CandA/1 proteins. We will especiallyemphasize whether DenA/1 interacts to the entire CSN or only to subcomplexes. A tagged RubA/Nedd8 recruits in csnE/Csn5 mutants but not in wildtype distinct components of cullin complexes and several associated proteins. We will analyse whether there is a specific CSN control of developmental-specific fungal cullin complexes and their putative substrates. Finally we will explore whether there is a crosstalk between the Nedd8 and the SUMO conjugation (or deconjugation) pathway during fruit body formation of the sexual cycle of this fungus, because a deletion of the gene for SUMO results in a similar developmental phenotype as a deneddylase deficient mutant of A. nidulans.