Light-dependent coordination of development and secondary metabolism in the filamentous fungus Aspergillus nidulans

丝状真菌构巢曲霉发育和次生代谢的光依赖性协调

• 批准号: 122777453
• 负责人: Professor Dr. Gerhard H. Braus
• 金额: --
• 依托单位: Abteilung für Molekulare Mikrobiologie und Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/122777453?language=en
• 关键词: Light; dependent; coordination; development; secondary

Secondary metabolism activities and developmental programs are interwoven in fungi and respond to numerous external stimuli. The focus of the proposed project is on the signal ‘light’. Illumination coordinately decreases in the filamentous model mold Aspergillus nidulans the production of sexual tissue and the synthesis of the secondary metabolite sterigmatocystin, a precursor of the highly cancerogenic aflatoxin. We will identify the proteins interacting to the UVA/blue light sensor CryA, a combined photolyase and cryptochrome. This includes the question, whether CryA communicates to other fungal light sensors via physical interaction. Transduction of the light signal reduces the amount of VeA within the nucleus, which is a component of the heterotrimeric nuclear velvet complex VelB/VeA/LaeA. This complex coordinates differentiation and secondary metabolite synthesis in the dark. The light-dependent VeA cis-located signal sequences and the trans-acting factors will be defined. Changes in the composition of the velvet complex during development will be monitored. We will also address, whether there are other interacting proteins to VelB and LaeA in strains lacking VeA in the absence or presence of light. The understanding of the developmental regulation of fungal secondary metabolism will help to control mycotoxin formation or increase the production of beneficial fungi-derived drugs.

次生代谢活动和发育程序在真菌中交织在一起，并对许多外部刺激做出反应。拟议项目的重点是信号“灯”。光照协同降低丝状模型霉菌构巢曲霉性组织的产生和次级代谢产物杂色曲霉素（高度致癌的黄曲霉毒素的前体）的合成。我们将鉴定与 UVA/蓝光传感器 CryA（光裂解酶和隐花色素的组合）相互作用的蛋白质。这包括 CryA 是否通过物理交互与其他真菌光传感器进行通信的问题。光信号的转导减少了细胞核内 VeA 的数量，VeA 是异源三聚体核绒复合物 VelB/VeA/LaeA 的组成部分。该复合物在黑暗中协调分化和次级代谢物合成。光依赖性 VeA 顺式定位信号序列和反式作用因子将被定义。将监测开发过程中天鹅绒复合物成分的变化。我们还将讨论在缺乏或存在光的情况下缺乏 VeA 的菌株中是否存在与 VelB 和 LaeA 相互作用的其他蛋白质。了解真菌次生代谢的发育调控将有助于控制霉菌毒素的形成或增加有益真菌衍生药物的产量。