Light-dependent coordination of development and secondary metabolism in the filamentous fungus Aspergillus nidulans

真菌的次级代谢活动和发育程序相互交织，并对许多外部刺激作出反应。建议项目的重点是信号灯。照明协调减少丝状模型霉菌构巢曲霉性组织的生产和合成的次级代谢产物杂色曲霉素，高度致癌的黄曲霉毒素的前体。我们将鉴定与UVA/蓝光传感器CryA相互作用的蛋白质，CryA是一种组合的光解酶和隐花色素。这包括CryA是否通过物理相互作用与其他真菌光传感器进行通信的问题。光信号的转导减少了细胞核内VeA的量，VeA是异源三聚体细胞核天鹅绒复合物VelB/VeA/LaeA的组分。这种复合物在黑暗中协调分化和次级代谢物的合成。将定义光依赖性VeA顺式定位信号序列和反式作用因子。将监测发育期间鹿茸复合物组成的变化。我们还将解决，是否有其他相互作用的蛋白VelB和LaeA在缺乏VeA的菌株在光的存在或不存在。了解真菌次生代谢的发育调控将有助于控制真菌毒素的形成或增加有益真菌衍生药物的产量。

Secondary metabolism activities and developmental programs are interwoven in fungi and respond to numerous external stimuli. The focus of the proposed project is on the signal ‘light’. Illumination coordinately decreases in the filamentous model mold Aspergillus nidulans the production of sexual tissue and the synthesis of the secondary metabolite sterigmatocystin, a precursor of the highly cancerogenic aflatoxin. We will identify the proteins interacting to the UVA/blue light sensor CryA, a combined photolyase and cryptochrome. This includes the question, whether CryA communicates to other fungal light sensors via physical interaction. Transduction of the light signal reduces the amount of VeA within the nucleus, which is a component of the heterotrimeric nuclear velvet complex VelB/VeA/LaeA. This complex coordinates differentiation and secondary metabolite synthesis in the dark. The light-dependent VeA cis-located signal sequences and the trans-acting factors will be defined. Changes in the composition of the velvet complex during development will be monitored. We will also address, whether there are other interacting proteins to VelB and LaeA in strains lacking VeA in the absence or presence of light. The understanding of the developmental regulation of fungal secondary metabolism will help to control mycotoxin formation or increase the production of beneficial fungi-derived drugs.