Study on negative ion-implanted biomaterials to induce vascular endothelial cells and neural cells

负离子植入生物材料诱导血管内皮细胞和神经细胞的研究

• 批准号: 08680926
• 负责人: SATO Hiroko
• 金额: $ 1.73万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680926/
• 关键词: negative ion implantation (NIABNIS); silver negative ions; tissue culture polystyrene; material surfaces; vascular endo-thelial cells; neural cells; hydrophilicity; hydrophobicitv; cell adhesion-proteins; 注入エネルギー; 接触角測定; 材料表面物性; 親水,疎水性; ポリスチレ

The purpose of this research is to study and develop on negative ion-implanted materials which assist and induce to repair and regenerate wounded biological tissues, such as vascular blood vessels and neural systems. Silver negative ions (Ag^-) were chosen first to be implanted to polystyrene (PS) and tissue culture polystyrene (TCPS) by using neutral and ionized alkaline bombardment-type heavy negative ion sources (NIABNIS) in doses of 10^<14> to 10^<16> ions "Jm^<-2> at relatively low ion energies of 5-30 keV.The surface property and biocompatability of Ag^--implanted TCPS and PS were investigated. Surfaces of Ag^--implanted PS were studied by means of secondary ion mass spectrometry (SIMS), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and micro-Raman spectroscopy, and contact angle measurement. As a result of Ag^--implantation, the polystyrene surfaces underwent degradation, thereby becoming more hydrophilic.The cell density of vascular endothelial cells (HUVEC) was observed to decrease remarkably on Ag^--implanted TCPS, compared with that on TCPS, while the cell density was observed to increase extremely on Ag^--implanted PS than on the untreated PS region. In addition, the cells were observed to be induced on Ag^--implanted PS region from PS region, and to be lined up in parallel on the TCPS region contiguous to the Ag^--implanted TCPS region in the shape of stripe. Murine-derived neural cells were observed to settle on the Ag^--implanted PS region in the manner same as vascular endothelial cells. In addition, the neurite outgrowth was also observed to develop specifically on the Ag^--implanted PS region. There was no significant difference for the culture of neural cells among on C^-, Cu^-, and Ag^--implanted PS and TCPS, although the mechanism for effects on cells is unclear and the further study is necessary.

本研究的目的是研究和开发负离子植入材料，以辅助和诱导修复和再生受损的生物组织，如血管和神经系统。采用中性和电离碱性轰击型重负离子源（NIABNIS），首先选择银负离子（Ag^-）注入聚苯乙烯（PS）和组织培养聚苯乙烯（TCPS），剂量为10^<14> ~ 10^<16>离子“Jm^<-2>”，离子能量相对较低，为5-30 keV。研究了Ag^-植入TCPS和PS的表面性能和生物相容性。采用二次离子质谱（SIMS）、x射线光电子能谱（XPS）、傅里叶变换红外光谱（FTIR）、微拉曼光谱（microraman spectroscopy， FTIR）和接触角测量等方法研究了Ag^-注入PS的表面。由于Ag^——的注入，聚苯乙烯表面发生降解，从而变得更亲水。Ag^-移植后血管内皮细胞（HUVEC）密度明显降低，而Ag^-移植后血管内皮细胞密度明显增加。此外，我们还观察到细胞从Ag^—植入的PS区诱导到PS区，并在与Ag^—植入的TCPS区相邻的TCPS区上平行排列成条形。小鼠来源的神经细胞以与血管内皮细胞相同的方式沉积在Ag^-植入的PS区。此外，还观察到神经突的生长特异性地发生在Ag^-植入的PS区。C^-、Cu^-和Ag^-植入的PS和TCPS对神经细胞的培养没有显著差异，但其对细胞的作用机制尚不清楚，需要进一步研究。

项目成果

H.Sato: "Surface modification of polystyrene surface by silver-negative ion implantation for improving surface wettability and cell adhesion property." Proc.the 8th Symp Beam Engin. Advanced Mater. Synthes. Includ. Bio-Med. Mater. & Treatment BEAMS1997. 1

H.Sato：“通过银负离子注入对聚苯乙烯表面进行表面改性，以提高表面润湿性和细胞粘附性能。”

H.Sato: "Improvement of neuron attachment properties of polystyrene by silver-negative implantation and induction of neural protrusion outgrowth." Proc.the 8th Symp Beam Engin. Advanced Mater. Synthes Includ. Bio-Med. Mater. & Treatment BEAMS1998. 87-90 (

H.Sato：“通过银阴性植入和诱导神经突起生长来改善聚苯乙烯的神经元附着特性。”

H.Sato: "Surface modification of polystyrene surface by silver-negative ion implantation for improving surface wettability and cell adhesion property." Proc.the 8th Symp.Beam Engin.Advanced Mater.Syntheses Includ.Bio-Med.Mater.& Treatment. BEAMS 1997. 109

H.Sato：“通过银负离子注入对聚苯乙烯表面进行表面改性，以提高表面润湿性和细胞粘附性能。”

佐藤弘子: "負イオン注入によるポリスチレンにおける神経細胞の接着特性の改質と分化誘導" 第9回粒子線の先端的応用技術に関するシンポジウム論文集. BEAMS 1998. 87-90 (1998)

Hiroko Sato：“通过负离子植入对聚苯乙烯中的粘附特性进行修改并诱导神经细胞的分化”第 9 届粒子束先进应用技术研讨会论文集 1998。87-90 (1998)。

H.Sato: "Negative-ion beam surface modification of tissue-culture polstyrene dishes for changing hydrophilic and cell-attachment properties." Nuclear Instr.Methods Phys.Res.(NIMB). B148. 1133-1140 (1999)

H.Sato：“负离子束表面修饰组织培养聚苯乙烯培养皿，以改变亲水性和细胞附着特性。”