In Vitro Studies on Human Endothelial Cells, Seeded on Materials for the purpose of Artificial Blood Vessels

人类内皮细胞的体外研究，接种在用于人造血管的材料上

• 批准号: 06680853
• 负责人: SATO Hiroko
• 金额: $ 1.41万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680853/
• 关键词: human vascular endothelial cells, HUVEC; artificial blood vessel; polymer materials; anti thrombogenicity; procoagulant; extracellular matrices; tissue plasminogen activator, tPA; plasminogen activator inhibitor type 1, PAI-1; tPA疎外タンパク質(PAH); ガス

Vascular endothelial cells contact with blood in vivo form no thrombi in the normal state. Artificial polymer materials where human vascular endothelial cells (HUVEC) are seeded may be expected to lead to antithrombogenic surfaces. Such hybrid materials will be useful as artificial blood vessels for a long-term implantation. Vascular endothelial cells, however, stimulated with cytokines, are known to change to the procoagulant state.In this study, the compatibility of HUVEC with artificial materials was investigated on fibrinolysis-related proteins, i.e., tissue-type plasminogen activator (tPA) and plasminogen inhibitor type 1 (PAI-1). The following results were obtained ;1) HUVEC were observed to be released relatively high amount of tPA in the initial culture period for four days.2) The activity of tPA,measured with a fluorogenic synthetic substrate, was scarcely detected in the culture medium of HUVEC,while the activity of tPA was detected in the HUVEC lysate component in which PAI-1 was inactivated. Probably, tPA,released from HUVEC,should form complex with PAI-1 in the culture medium.3) The ratio of amount of tPA against that of PAI-1, released in the culture medium, was dependent on extracellular matrices (ECM), i.e., ECM proteins and artificial materials used.

血管内皮细胞在正常状态下与体内血液接触不形成血栓。人工聚合物材料，其中人类血管内皮细胞（HUVEC）接种可能会导致抗血栓形成的表面。这样的混合材料将可用作长期植入的人造血管。然而，已知血管内皮细胞在细胞因子刺激下会转变为促凝血状态。在本研究中，研究了HUVEC与人工材料的相容性，研究了与纤维蛋白溶解相关的蛋白，即，组织型纤溶酶原激活剂（tPA）和纤溶酶原抑制剂1型（派-1）。结果表明：（1）HUVEC在培养初期4 d内释放出相对较高的tPA;（2）用荧光合成底物测定，在HUVEC培养液中几乎检测不到tPA的活性，而在派-1失活的HUVEC裂解液组分中检测到tPA的活性。3）培养液中tPA与派-1的释放量之比依赖于细胞外基质（ECM），即细胞外基质（ECM）浓度的高低，而tPA与派-1的释放量之比则依赖于细胞外基质浓度的高低，即细胞外基质浓度的高低。使用ECM蛋白和人工材料。

项目成果

佐藤弘子: "人工材料に接着したヒト血管内皮細胞の増殖および細胞状態に関する研究(II)" 生体材料. 13. 160-166 (1995)

Hiroko Sato：“附着于人造材料的人血管内皮细胞的增殖和细胞状态的研究（II）”Biomaterials 13. 160-166（1995）。

Hiroko Sato and Kazuya Mori: "Compatibility of vascular endothelial cells with a thromboresistant artificial material" J.Japanese Soc.Thrombosis and Hemostasis. 6-4. 272-278 (1995)

Hiroko Sato 和 Kazuya Mori：“血管内皮细胞与抗血栓人造材料的相容性”J.Japan Soc.Thrombosis and Hemostasis。

佐藤弘子: "人工材料に対するヒト血管内皮細胞の接着および増殖に関する研究(I)" 生体材料. 13. 105-111 (1995)

佐藤博子：“人血管内皮细胞对人造材料的粘附和增殖的研究（I）”生物材料。 13. 105-111（1995）。

佐藤弘子,森和哉: "血管内皮細胞の抗血栓性人工材料に対する適合性" 日本血栓止血学会誌. 6. 272-278 (1995)

Hiroko Sato、Kazuya Mori：“血管内皮细胞对抗血栓人造材料的适用性”日本血栓和止血学会杂志 6. 272-278 (1995)。

Hiroko Sato and Kazuya Mori: "A study on proliferation and cell states of human vascular endothelial cells, attached on artificial surfaces (II)" Seitai-Zairyou (Biomaterials). 13-4. 160-166 (1995)

Hiroko Sato 和 Kazuya Mori：“关于附着在人造表面上的人类血管内皮细胞的增殖和细胞状态的研究 (II)”Seitai-Zairyou（生物材料）。