课题基金 / 基金详情

Transposition mechanism of DNA-type transposable elements of the medaka fish

Transposition mechanism of DNA-type transposable elements of the medaka fish
青鳉鱼DNA型转座元件的转座机制
批准号:
09680672
负责人:
KOGA Akihiko
金额:
$1.54万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998

项目摘要

项目成果

KOGA Akihiko的其他基金

相似基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
For the purpose of studying the transposition mechanisms of the medaka fish transposable element Tol2, we first established an experimental system to obtain the excision rate of the element, in which the bacterial lacZ gene on a plasmid vector was used as an indicator of excision. In this system, loss of the Tol2 element due to its excision results in blue bacterial colonies. Next, using this system, we identified an autonomous Tol2 copy, Tol2 is present in the genome of the medaka fish (O.latipes) but is not in the Luzon medaka fish (O.luzonensis) which is closely related to the medaka fish. Therefore, excision of a single element introduced into the Luzon medaka fish indicates that the element is autonomous. It was successful to detect excision, and, thus, the copy used was found to be an autonomous element.After these preparations, we started examining portions of the autonomous Tol2 copy for their importance on the transposition function. We made various clones that carry sequence alterations or deletions, and estimated the transposition rate of the clones. The following results were obtained : (1) The terminal inverted repeat of the Tol2 element are required for transposition. (2) A factor necessary for transposition is present in the central region of the element. (3) The length of the element is positively correlated with the transposition rate. The last two things are unique to Tol2, that is, other transposable elements so far examined do not have these characters.We also examined variation in the structure of Tol2 elements in a medaka fish natural population. Tol2 was found to be highly homogeneous in length and the structure. This is consistent with the results about the transposition mechanisms described above.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
KOGA,Akihiko: "Albinism due to transposable element insertion in fish" Pigment Cell Research. 10(6). 377-381 (1997)
KOGA,Akihiko:“由于鱼体内插入转座元件而导致白化病”色素细胞研究。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Kawakami, K., Koga, A., Hori, H.and Shima, A.: "Excision of the Tol2 transposable element of the medaka fish, Oryzias latipes, in zebrafish, Danio rerio." Gene. 225(1). 17-22 (1998)
Kawakami, K.、Koga, A.、Hori, H. 和 Shima, A.:“在斑马鱼 Danio rerio 中切除青鳉鱼 Oryzias latipes 的 Tol2 转座元件。”
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
KAWAKAMI,Koichi: "Excision of the Tol2 transposable element of the medaka fish,Oryzias latipes,in zebrafish,Danio rerio." Gene. 225(1). 17-22 (1997)
KAWAKAMI、Koichi:“在斑马鱼、斑马鱼中切除青鳉鱼、Oryzias latipes 的 Tol2 转座元件。”
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
9
    Rapid karyotypic changes in a primate taxon: possibility of participation of repetitive DNA as chromosome breakage inducer
    • 批准号:
      25650152
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.58万
    • 财政年份:
      2013
    • 负责人:
      KOGA Akihiko
    • 依托单位:
    Fluctuation in the size of species-specific repetitive DNA of hominoids: quantitative analysis by comparative genomics
    • 批准号:
      24370098
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $10.98万
    • 财政年份:
      2012
    • 负责人:
      KOGA Akihiko
    • 依托单位:
    Extinction of constitutive heterochromatin blocks in humans: structural analysis for clarification of mechanisms
    • 批准号:
      23657165
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.33万
    • 财政年份:
      2011
    • 负责人:
      KOGA Akihiko
    • 依托单位:
    Factors causing sudden increase in the transposition frequency of transposable elements in medaka fish
    海外基金