Relationship between the expression of cell adhesion molecule and tumor invasion and metastasis in primary oral squamous cell carcinomas

原发性口腔鳞癌细胞粘附分子表达与肿瘤侵袭转移的关系

• 批准号: 09672083
• 负责人: SHIRASU Rikiya
• 金额: $ 1.54万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672083/
• 关键词: Oral squamous cell carcinoma; Intercellular adhesion; Cell adhesion molecule; Invasion; Metastasis; Cadherin; Catenin; 口腔原発癌; 細胞間接着; 口腔原発扁平上皮癌; 浸潤能; 腫瘍分化度; E型カドヘリン; α-カテニン

We carried out immunohistochemical investigations on the relationship of E-cadherin expression to prognosis in patients with primary oral squamous cell carcinoma.E-cadherin is expressed homogeneoulsy with a typical membranous staining at cell-cell borders in normal oral epithelium. Decreased (or lost) expression of E-cadherin closely with cancer differentiation grade and with cancers having clinical lymph node metastasis. Moreover, decreased (or lost) E-cadherin expression correlated with shorter patient survival. However, E-cadherin expression did not correlate with T-category or clinical stage.Intercellular adhesion of epithelial tissue is mainly regulated by the E-cadherin (E-CD) molecule, α-Catenin (α-CAT) is an E-CD associated cytoplasmic protein that forms a linkage to the cytoskeleton and regulates E-CD function. To investigate the relationship between the expression of E-CD and α-CAT, and the expression of both adhesion molecules and histopathological features, we examined immu … More nohistochemically and biochemically E-CD and α-CAT expression in primary oral squamous cell carcinoma biopsies. Both adhesion molecules expressed homogeneously with a typical membranous staining at cell-cell borders in normal oral epithelium.The reduction (or loss) of E-CD expression was 38.7%, and that of α-CAT was 71.0% in oral cancers. The expression of α-CAT was closely correlated with that of E-CD in many cancers, but the reduction (or loss) of α-CAT expression was greater than E-CD in some. Regarding histopathological features, the reduction (or loss) of α-CAT expression is more correlated with cancer differentiation and invasion than is E-CD expression in human oral squamous cell carcinomas.These results suggest that not only E-CD but also α-CAT are important regulators of intercellular adhesion, and that α-CAT is also involved in tumor differentiation and invasiveness. These findings suggest that down-regulation of α-catenin, which may reflect dysfunction of the cadherin-mediated cell-cell adhesion system, is a parameter of lymph node metastasis in patients with oral squamous cell carcinoma. Less

我们对原发性口腔鳞状细胞癌患者E-cadherin表达与预后的关系进行了免疫组化研究。在正常口腔上皮中，e -钙粘蛋白均质表达，细胞-细胞边界呈典型的膜性染色。e -钙粘蛋白表达的减少（或缺失）与肿瘤分化程度和临床淋巴结转移密切相关。此外，E-cadherin表达减少（或缺失）与患者生存期缩短相关。然而，E-cadherin的表达与t类型或临床分期无关。上皮组织的细胞间粘附主要受E-cadherin （E-CD）分子调控，α-Catenin （α-CAT）是E-CD相关的细胞质蛋白，与细胞骨架形成连锁，调控E-CD功能。为了探讨E-CD和α-CAT的表达以及粘附分子的表达与组织病理特征的关系，我们检测了原发性口腔鳞状细胞癌组织中E-CD和α-CAT的非组织化学和生化表达。两种粘附分子均表达，在正常口腔上皮细胞边界呈典型的膜样染色。口腔癌中E-CD和α-CAT的表达分别降低（或丢失）38.7%和71.0%。在许多肿瘤中α-CAT的表达与E-CD的表达密切相关，但在某些肿瘤中α-CAT表达的降低（或丧失）大于E-CD。在组织病理学特征方面，α-CAT表达的降低（或缺失）比E-CD表达更与人口腔鳞状细胞癌的分化和侵袭相关。这些结果表明，E-CD和α-CAT都是细胞间粘附的重要调节因子，α-CAT还参与了肿瘤的分化和侵袭。提示α-catenin的下调可能反映了钙粘蛋白介导的细胞-细胞粘附系统功能障碍，是口腔鳞状细胞癌患者淋巴结转移的一个参数。少

项目成果

SAKAKI T: "Expression of E-Catherine and α-catenin in primary oral squamous cell carcinomas"Japanese Journal of Oral and Maxillofacial Surgery. 44-1. 1-10 (1998)

SAKAKI T：“E-Catherine 和 α-catenin 在原发性口腔鳞状细胞癌中的表达”日本口腔颌面外科杂志 44-1（1998）。

榊 敏男: "口腔扁平上皮癌におけるE型カドヘリンとα-カテニンの発現"日本口腔外科学会雑誌. 44. 1-10 (1998)

Toshio Sakaki：“E型钙粘蛋白和α-连环蛋白在口腔鳞状细胞癌中的表达”日本口腔颌面外科杂志44. 1-10 (1998)。

SAKAKI T: "Expression of cadherin-catenin complex in primary oral squamous cell carcinomas with cervical lymph node metastasis"Journal of the Japanese Stomatological Society. 48-6. 447-453 (1999)

SAKAKI T：“钙粘蛋白-连环蛋白复合物在原发性口腔鳞状细胞癌伴颈淋巴结转移中的表达”日本口腔医学会杂志。

SAKAKI T: "Correlation of E-cadherin and α-catenin expression with differentiation of oral squamous cell carcinoma"Journal of Osaka Dental University. 33-2. 75-81 (1999)

SAKAKI T：“E-钙粘蛋白和α-连环蛋白表达与口腔鳞状细胞癌分化的相关性”大阪牙科大学杂志33-2（1999）。

榊 敏男: "口腔原発癌の頸部リンパ節転移とカドヘリン-カテニン複合体の発現性"日本口腔科学会雑誌. 48. 447-453 (1999)

Toshio Sakaki：“原发性口腔癌的颈部淋巴结转移和钙粘蛋白-连环蛋白复合物的表达”日本口腔医学会杂志 48. 447-453 (1999)。