INTERALEUKIN-8 AS AN EARLY MARKER OF BACTERIAL INFECTION

细胞因子网络在感染性疾病的发病机制中被激活。我们检测了152例经培养证实或怀疑为败血症的患者和25名对照组的血清白细胞介导性细胞因子IL-8的浓度。败血症患者血清IL-8水平明显高于正常对照组。血培养阴性的脓毒症患者IL-8浓度显著高于对照组。此外，还对6例急性髓系白血病化疗患者的血清IL-8水平进行了前瞻性检测。在发烧和C反应蛋白升高前1天，IL-8浓度升高。最后，用佛波酯(PMA)、脂多糖(LPS)或脂磷壁酸(LTA)刺激人外周全血细胞，研究细胞因子释放的调节作用。逆转录-聚合酶链式反应检测PMA或LPS刺激10min后IL-8mRNA的表达，PMA、LPS或LTA刺激3h后IL-8分泌到培养上清液中。这些数据表明，IL-8是细菌感染的一个有用的早期指标。

The cytokine network is activated in the pathogenetic mechanisms of septic diseases. We measured serum concentrations of interleukin(IL)-8, a leukocyte-mediated cytokine, in 152 patients with culture-proven or suspected septicemia and 25 control subjects. The IL-8 concentrations in patients who were diagnosed as septicemia were significantly higher than those of controls. IL-8 concentrations in septic patients with negative blood cultures were significantly higher than in controls. In addition, serum IL-8 concentrations were assayed prospectively in 6 patients undergoing chemotherapy for acute myelogenous leukemia. Increased IL-8 concentrations preceded onset of fever and elevation of C-reactive protein by 1 day. Finally, to investigate modulation of cytokine release in vitro, human peripheral whole blood cells were stimulated with phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS), or lipoteichoic acid (LTA). IL-8 mRNA expression was detected by reverse transcription-polymerase chain reaction 10min after PMA or LPS exposure, and IL-8 was secreted into culture media 3 hr after PMA, LPS, or LTA stimulation. These data suggest that LL-8 is a useful early indicator of bacterial infection.