INTERALEUKIN-8 AS AN EARLY MARKER OF BACTERIAL INFECTION

INTERALEUKIN-8 作为细菌感染的早期标志物

• 批准号: 09672350
• 负责人: KANDA Tsugiyasu
• 金额: $ 1.98万
• 依托单位: GUNMA UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672350/
• 关键词: INTERLEUKIN-8; INFECTION; DIAGNOSIS; インターロイキン8; 敗血症; インターロイキン6; ウエスタンブロッテイング; サイトカイン

The cytokine network is activated in the pathogenetic mechanisms of septic diseases. We measured serum concentrations of interleukin(IL)-8, a leukocyte-mediated cytokine, in 152 patients with culture-proven or suspected septicemia and 25 control subjects. The IL-8 concentrations in patients who were diagnosed as septicemia were significantly higher than those of controls. IL-8 concentrations in septic patients with negative blood cultures were significantly higher than in controls. In addition, serum IL-8 concentrations were assayed prospectively in 6 patients undergoing chemotherapy for acute myelogenous leukemia. Increased IL-8 concentrations preceded onset of fever and elevation of C-reactive protein by 1 day. Finally, to investigate modulation of cytokine release in vitro, human peripheral whole blood cells were stimulated with phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS), or lipoteichoic acid (LTA). IL-8 mRNA expression was detected by reverse transcription-polymerase chain reaction 10min after PMA or LPS exposure, and IL-8 was secreted into culture media 3 hr after PMA, LPS, or LTA stimulation. These data suggest that LL-8 is a useful early indicator of bacterial infection.

细胞因子网络在脓毒性疾病的发病机制中被激活。我们测量了152例培养证实或疑似败血症患者和25例对照组的血清白细胞介素(IL)-8（一种白细胞介导的细胞因子）浓度。诊断为败血症的患者IL-8浓度明显高于对照组。血培养阴性脓毒症患者IL-8浓度明显高于对照组。此外，对6例急性髓性白血病化疗患者血清IL-8浓度进行了前瞻性测定。IL-8浓度升高比发热和c反应蛋白升高早1天。最后，为了研究细胞因子在体外释放的调节，用12-肉豆蔻酸酯（PMA）、脂多糖（LPS）或脂质胆酸（LTA）刺激人外周血细胞。在PMA或LPS刺激后10min通过逆转录聚合酶链反应检测IL-8 mRNA表达，并在PMA、LPS或LTA刺激后3h将IL-8分泌到培养基中。这些数据表明，LL-8是细菌感染的一个有用的早期指标。

项目成果

神田亨勉 中野正幸 小林功: "Heat stress aggravates viral myocarditis" Life Science. 164・2. 93-101 (1999)

Totsutomu Kanda、Masayuki Nakano、Isao Kobayashi：“热应激会加重病毒性心肌炎”《生命科学》164・2。