ISOLATION AND CHARACTERIZATION OF CYTOKINES SECRETED FROM CARDIAC MYXOMAS AND APPLICATION FOR CONGESTIVE HEART FAILURE

心脏粘液瘤分泌的细胞因子的分离和表征及其在充血性心力衰竭中的应用

• 批准号: 07670752
• 负责人: KANDA Tsugiyasu
• 金额: $ 0.38万
• 依托单位: GUNMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670752/
• 关键词: interleukin-6; interleukin-8; cardiac myxoma; myocarditis; ウイルス; インターロンキン-6; インターロンキン-8; ウイルス性心筋炎; 心不全

We investigated the structure and charactrization of cytokines secreted from cardiac myxomas and analyzed the role of these cytokines for cangestive heart failure. Among eighteen cases of cardiac myxomas, 15 cases (83%) wass positively stained with antiinterleukin-6 (IL-6) antibody. Patients with IL-6 positive cardiac myxomas showed autoimmune like symptoms, such as weghtoloss, easy fatigability and fever. Their laboratory findings revealed higher levels of immunoglobulin G and A or gamma-globulin. Cytokines secreted from cardiac myxoma was analyzed by Western blotting. The molecular weight was about 10 to 12 kDa. This band was positive with anti-interleukin-8 (IL-8) antibodies.Cultured supernatants obtained from cardiac myxomas inproved the survival of mice infected with encephalomyocarditis virus (EMCV). We compared the effects for viral myocarditis inbetween IL-6 and IL-8. IL-6, not IL-8, was beneficial for viral myocarditis of mice. Histopathological examination and viral analysis showed the reduction of myocarial necrosis by IL-6.Therefore, we hypohtesize that IL-6 secreted from cardiac myxoma induces autoimmune like symptoms in patients with cardiac myxoma and that IL-6 is beneficial for the reduction of cardiac myxomas of mice induced from EMCV.

我们研究了心脏粘液瘤分泌的细胞因子的结构和特征，并分析了这些细胞因子在充血性心力衰竭中的作用。 18例心脏粘液瘤中，15例（83%）抗白细胞介素6（IL-6）抗体染色呈阳性。 IL-6阳性心脏粘液瘤患者表现出体重减轻、易疲劳、发热等自身免疫样症状。他们的实验室结果显示免疫球蛋白 G 和 A 或丙种球蛋白水平较高。通过蛋白质印迹分析心脏粘液瘤分泌的细胞因子。分子量约为10至12kDa。该条带呈抗白细胞介素 8 (IL-8) 抗体阳性。从心脏粘液瘤获得的培养上清液提高了感染脑心肌炎病毒 (EMCV) 的小鼠的存活率。我们比较了 IL-6 和 IL-8 对病毒性心肌炎的作用。 IL-6（而非 IL-8）对小鼠病毒性心肌炎有益。组织病理学检查和病毒分析显示IL-6可减少心肌坏死。因此，我们推测心脏粘液瘤分泌的IL-6可诱导心脏粘液瘤患者出现自身免疫样症状，并且IL-6有利于减少EMCV诱导的小鼠心脏粘液瘤。

项目成果

神田享勉他: "Modification of viralmyocarditis by interleukin-6" Circ.Res.78・5. 848-856 (1996)

Kanda京都等人：“白细胞介素6对病毒性心肌炎的修饰”Circ.Res.78・5（1996）。

神田享勉 他: "Modification of viralmyocarditis by interleukin-6" Circ.Res.78・5. 848-856 (1996)

Kanda 等：“白细胞介素 6 改变病毒性心肌炎”Circ.Res.78・5（1996）。

Kanda-T,McManus-JW,Imai-S,Decheng Yang, McManus-BM,Suzuki-T,Nagai-R,Kobayashi-I.: "Modification of viral myocarditis by interleukin-6" Circ-Res. 78. 848-856 (1996)

Kanda-T、McManus-JW、Imai-S、De Cheng Yang、McManus-BM、Suzuki-T、Nagai-R、Kobayashi-I.：“白细胞介素 6 对病毒性心肌炎的修饰”Circ-Res。

Kanda-T,Sakamoto-H,McManus-BM,Sakamaki-T,Suzuki-T,Nagai-R,Kobayashi-I.: "Interleukin-6 secreted from human myxoma reduces murine viral myocarditis." Life-Sci. 58. 1705-1712 (1996)

Kanda-T、Sakamoto-H、McManus-BM、Sakamaki-T、Suzuki-T、Nagai-R、Kobayashi-I.：“人粘液瘤分泌的白细胞介素 6 可减少小鼠病毒性心肌炎。”

神田享勉他: "Interleukin-6 secreted from human myxoma reduces murine myocarditis" Life Sci.58・20. (1996)

Kanda京都等人：“人粘液瘤分泌的白细胞介素6减少小鼠心肌炎”Life Sci.58・20（1996）。