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Effects of Hypoxic Stimuli on Bronchopulmonary Neuroepithelial Bodies (Paraneuron)

缺氧刺激对支气管肺神经上皮体（副神经元）的影响

基本信息

批准号：
09671544
负责人：
SATO Kazunori
金额：
$ 1.79万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Bronchopulmonary neuroepithelial bodies (NEBs), composed of inervated clusters of amine-and peptide-containg cells, are widely distributed throughout the airway mucosa of human and animal lungs. Structuary, NEBs resemble chemoreceptors such as corotid bobies, taste buds and are thought to function as hypoxia senstive airway sensors. Using histochemical and histoimmunochemichal thechniques, NEGs have been shown to contain serotonine (5-HT) and peptidelike substances in their cytoplasmic vesicles. Under alveolar hypoxia, NEBs have been shown to release the vesicles to intercellular space. Though the mechanism of oxygen sensing by these cells and the physiological roles of the release of the cytoplasmic vesicles are unkown, we hypothesize that the release of vesicles which contain 5-HT play essential roles in the responses of pulmonary vascular constriction to inhalational hypoxia (hypoxic pulmonary vasoconstriction, HPV). In this study, we used isolated lung perfusion models of rats and … More investigated the effects of inhalational anesthetics, sevoflurane and isoflurane, and hypothermia on the responses of NEBs to hypoxia or HPV. We also examined the effects of partial liquid ventilation, a new ventilatory supporting thechnique for the managiment of sever respiratory failure, on HPV in the isolated lung perfusion models.Both inhalational anesthetics sevoflurane and isoflurane inhibited HPV dose dependently. Inhalational concentration of 4.0% sevoflurane and 3.0% isoflurane completely blocked the response. But the response of pulmonary vessels to hypoxia was recovered at 60 minutes after ceassation of inhalation. The inhibitory effects of the anesthetics on HPV were reversible. HPV was also inhibited by hypothermia. When the temprature of perfusate was below 30 degrees of centigrade, HPV was completely blocked. According to the increase of the temprature of perfusate above 33 degrees of Centigrade, HPV was restored. HPV was not affected by perflubrone associated partial liquid ventilation. Less

支气管肺神经上皮小体（neuroepithelial bodies，NEB）广泛分布于人和动物肺的气道粘膜中，由含胺和肽的神经细胞团组成。结构上，NEB类似于化学感受器，如颈动脉、味蕾，并被认为是缺氧敏感的气道传感器。利用组织化学和组织免疫化学技术，已证明NEGs的胞浆囊泡中含有5-HT和肽样物质。在肺泡缺氧的情况下，NEB已经被证明可以将囊泡释放到细胞间隙。虽然这些细胞的氧传感机制和释放的细胞质囊泡的生理作用是未知的，我们推测，含有5-HT的囊泡的释放在肺血管收缩对吸入性缺氧（缺氧性肺血管收缩，HPV）的反应中起着至关重要的作用。在这项研究中，我们使用大鼠离体肺灌注模型， ...更多信息研究了吸入麻醉药七氟醚和异氟醚以及低温对NEB对缺氧或HPV反应的影响。在离体肺灌流模型上，我们还观察了部分液体通气（一种新的治疗严重呼吸衰竭的辅助技术）对HPV的影响，吸入麻醉药七氟醚和异氟醚均能剂量依赖性地抑制HPV。吸入浓度为4.0%的七氟醚和3.0%的异氟醚可完全阻断该反应。但停止吸入后60分钟，肺血管对缺氧的反应即可恢复。麻醉药对HPV的抑制作用是可逆的。HPV也被低温抑制。当灌流液温度低于30 ℃时，HPV被完全阻断。当灌流液温度升高到33 ℃以上时，HPV即恢复。HPV不受perflubrone相关部分液体通气的影响。少