Studies on role of the stromal cell which regulates the histology

基质细胞调节组织学作用的研究

• 批准号: 09671638
• 负责人: NOGUCHI Sumio
• 金额: $ 0.83万
• 依托单位: YOKOHAMA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671638/
• 关键词: PROSTATE CANCER; STROMAL CELL; INTERLEUKIN-6; CMA; 組織型

We focused on stromal component which was abundantly included for the poorly differentiated prostatic cancer (column and cord) and the direct effect of antiandrogen(CMA) to the prostatic stromal cells. Stromal cell proliferation in serum free culture was dose-dependently inhibited by CMA and IL-6 recovered these inhibitory effects. IL-6 concentration in conditioned medium of stromal cells was decreased by CMA(10-9 M〜10-6M). IL-6 mRNA were present in stromal cells from prostatic tissues but CMA did not effect the expression of mRNA level.From these results, it was indicated that IL-6 release to the CM decreased by lowering the permeability of the cell membrane of stromal cells by CMA.

我们集中在低分化前列腺癌(柱状和索状)中含有丰富的间质成分，以及抗雄激素(CMA)对前列腺间质细胞的直接作用。CMA可剂量依赖性地抑制无血清培养的基质细胞增殖，而IL-6可恢复这种抑制作用。CMA(10~(-9)M~10~(-6)M)可降低基质细胞条件培养液中IL-6浓度。IL-6在前列腺间质细胞中有表达，而CMA对其表达水平无影响，提示CMA通过降低间质细胞膜通透性而减少向CM释放IL-6。

项目成果

Noguchi S: "Pathologic "jused gland" as a prognostic factor for prostate cancer"Urologia Internationalis. (in press). (2000)

野口 S：“病理性‘判断腺’作为前列腺癌的预后因素”《Urologia Internationalis》。

Masuda,M: "Prognostic significance of Ki-67,p53 and Bcl-2 expression in prostate cancer patiens with lymphode metastasis" Pathology International. 48. 41-46 (1998)

Masuda,M：“Ki-67、p53 和 Bcl-2 表达在前列腺癌淋巴结转移患者中的预后意义”国际病理学。

Masuda M: "Drognostic significance of ki-67, p53, and BC1-2 expression in prostate cancer patients with lymphnode matastasis"Pathology International. 48. 41-46 (1998)

Masuda M：“伴有淋巴结转移的前列腺癌患者中 ki-67、p53 和 BC1-2 表达的诊断意义”国际病理学。

Masuda M, Takano Y, Iki M, Asakura T, Hashiba T, Noguchi S, Hosaka M: "Prognostic significance of Ki-67, p53, And Bcl2 expression in prostate cancer patients with lymph Node metastases"Pthol. Int.. 48. 41-46 (1998)

Masuda M、Takano Y、Iki M、Asakura T、Hashiba T、Noguchi S、Hosaka M：“Ki-67、p53 和 Bcl2 表达在前列腺癌淋巴结转移患者中的预后意义”Pthol。

Masuda M: "The worst histologic elements predict progrosis in moderately-differentiated prostate cancer patients with androgen with drawal therapy"European Urology. 36. 197-202 (1999)

Masuda M：“最差的组织学因素预测中分化前列腺癌患者在雄激素停药治疗中的进展”欧洲泌尿学。