Immunohistological Studies about the Role of betaig-h3 during the Wound Healing of the Corncal Stroma

betaig-h3 在角膜基质伤口愈合过程中作用的免疫组织学研究

• 批准号: 09671790
• 负责人: HIRANO Koji
• 金额: $ 1.79万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671790/
• 关键词: corneal subepithelial tissue; bullous keratopathy; betaig-h3; basement membrane; Fuchs'endothelial dystrophy; βig-h3

We studied the histopathology of both the primary eudothelial dystrophic (Fuchs' endothelial dystrophy) and the secondary induced bullous keratopathy conreas. Au additional layer was seen between the epithelium and Bowman's layer by the light microscopy. Examination of electron microscopy showed thickened basement membrane and fibrous tissue, and sometimes multiplication of the basement membrane in the extra layer in some specimens. There is no difference between the Fuchs' dystrophic corneas and surgically induced bullous keratopathy corneas. The subepithelial tissue of bullous keratopathy corneas may be formed by epithelium and stromal cells that have migrated into the subepithelial area of the diseased corneas. This morphological abnormality in the BK corneas may induce the detachment of the epithelium and form the bullac. beta ig-h3 is supposed to contribute to the adhesion of the epithelium and the Bowman's layer, and the disfunction of the protein causes the epithelial disturbance. To search for the clinical application of the novel protein, beta ig-h3, This study focused on the pathology of the bullous keratopathy corneas which is the most common conical epithelial disorder.

我们研究了原发性内皮营养不良（Fuchs' endothelial dystrophy）和继发性大泡性角膜病变角膜的组织病理学。光镜下见上皮与Bowman层之间有Au附加层。电镜下可见基底膜和纤维组织增厚，部分标本外层基底膜增生。Fuchs的营养不良角膜和手术诱导的大泡性角膜病变角膜之间没有差异。大泡性角膜病变角膜的上皮下组织可由上皮细胞和已迁移到患病角膜的上皮下区域的基质细胞形成。BK角膜的这种形态学异常可能导致上皮脱落并形成泡状突起。β ig-h3被认为有助于上皮和Bowman层的粘附，并且该蛋白的功能障碍引起上皮紊乱。为了探索β ig-h3蛋白的临床应用，本研究对大泡性角膜病变这一最常见的角膜圆锥上皮病变进行了病理学研究。

项目成果

Hirano K and Sai S: "Severe Acanthamoeba sclerokeratitis in a non-contact lens wearer." Acta Ophthalmol Scandinav. 76(in press). (1999)

Hirano K 和 Sai S：“非隐形眼镜佩戴者患有严重棘阿米巴硬化性角膜炎。”

Hirano K: "Intrafibrillar Matrices in the Corneal Stroma." Current Opinion in the Kyoto Cornea Club vol.3,1999, Kugler Publications Bv, Amsterdam. (In press).

Hirano K：“角膜基质中的纤维内基质。”

Hirano K and Miyagawa A: "Corneal Subepithelial Tissue of Fuchs' Endothelial Dystrophy." Folia Ophthalmol Jpn. 48. 1292-1295 (1997)

Hirano K 和 Miyakawa A：“福克斯内皮营养不良的角膜上皮下组织”。