Immunohistological Studies about the Role of betaig-h3 during the Wound Healing of the Corncal Stroma

我们研究了原发性上皮营养不良(Fuchs内皮营养不良)和继发性大泡性角膜病变的组织病理学改变。光镜下可见上皮与Bowman‘s层之间有Au附加层。电子显微镜检查可见基底膜增厚，纤维组织增厚，有时基底膜增厚，部分标本基底膜增厚。Fuchs‘s营养不良的角膜和手术导致的大泡性角膜病变之间没有区别。大泡性角膜病变的上皮下组织可能是由迁移到病变角膜上皮下区的上皮和基质细胞形成的。BK角膜的这种形态异常可能导致上皮细胞脱离，形成大疱性角膜泡。βig-h3被认为促进了上皮细胞与鲍曼层的粘连，而该蛋白的功能障碍导致了上皮细胞的紊乱。为了探索新型蛋白βig-h3的临床应用，本研究对最常见的圆锥上皮病变大泡性角膜病变的病理机制进行了研究。

We studied the histopathology of both the primary eudothelial dystrophic (Fuchs' endothelial dystrophy) and the secondary induced bullous keratopathy conreas. Au additional layer was seen between the epithelium and Bowman's layer by the light microscopy. Examination of electron microscopy showed thickened basement membrane and fibrous tissue, and sometimes multiplication of the basement membrane in the extra layer in some specimens. There is no difference between the Fuchs' dystrophic corneas and surgically induced bullous keratopathy corneas. The subepithelial tissue of bullous keratopathy corneas may be formed by epithelium and stromal cells that have migrated into the subepithelial area of the diseased corneas. This morphological abnormality in the BK corneas may induce the detachment of the epithelium and form the bullac. beta ig-h3 is supposed to contribute to the adhesion of the epithelium and the Bowman's layer, and the disfunction of the protein causes the epithelial disturbance. To search for the clinical application of the novel protein, beta ig-h3, This study focused on the pathology of the bullous keratopathy corneas which is the most common conical epithelial disorder.