Molecular analysis of pathogen in periodontopathic bacteria

牙周病细菌病原体的分子分析

• 批准号: 09671871
• 负责人: ISHIHARA Kazuyuki
• 金额: $ 1.28万
• 依托单位: TOKYO DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671871/
• 关键词: Protease; Hemolytic activity; Treponema denticola; Fimbriae; Expression regulation; Actinobacillus actinomycetemcomitans; Pathogenicity; Bacteroides forsythus; fimbriae; hemolytic activity; expression regulation; Cloning; Fimhrial; Sequence /

Adult periodontitis was Infectious disease and it induce tooth loss. It cause a mastication problem and finally induce poor quality of life in patients with periodontal disease. As many attempts to clarify pathogen of the disease have been performed, the molecular mechanisms by which these pathogens exert their effects are poorly understood. To clarify the pathogenic mechanism of potential periodontopathic bacteria, we tried to characterize genes encoding pathogens from Actionbacillus actinomycetemcomitans, Bacteriodesforsydius and Treponema denticola.We determined the fimbriae gene (fap) of A.actinomycetemcomitans to characterize its role in colonization. The fimbriated strain of this microorganism lost fimbriae upon passaging onto solid medium. W found that expression of the fap gene was decreased in non-fimbriated strain (Microbiol. Pathog. 1997, 23 : 63-69).Proteases were known to exert a cytotoxic effects to host tissue. We detennined the sequence of a protease gene (prH) of B.for … More sythus which is difficult to get enough growth in synthetic medium for a characterization. A molecular mass of the protease is 47,873 and the PrtH possessed hemolytic activity (Imfect. Immun. 1997, 65 : 4888-4891).Several reports indicated the systemic chemotherapy failure was caused by colonization of Helicobcterpylori in oral cavity. We evaluated the antagonistic effects between oral microorganism and H.pylon. H.pylon was coaggregated with P.gingivalis and F.nucreatum. On the other hand, several oral bacteria inhibits growth of H.pylon (FEMS Microbial. Lett. 1997, 152 : 355-361).Evaluation of a pathogenicity using wild type strain and single gene deficient mutant is the most effective way to evaluate its pathogenicity. We constructed a knockout mutant of the prolyl-phenylalanine specific protease (dentilisin) in T denticola and characterized the mutant. Surface hydrophobicity of this microorganism decreased dramatically and ability of abscess formation decreased in the mutant. In addition, we found that dentilisin is associated with organization of outer sheath protein. These results indicated that dentilisin exert a cytotoxic effects to host tissue directly (J.Bacteriol. 1998, 180 : 3837-38449). Less

成人牙周炎是一种传染病，会导致牙齿脱落。它会导致咀嚼问题，最终导致牙周病患者的生活质量下降。尽管已经进行了许多澄清该疾病病原体的尝试，但这些病原体发挥其作用的分子机制仍知之甚少。为了阐明潜在牙周病细菌的致病机制，我们尝试鉴定编码放线杆菌、Bacteriodesforsydius和齿垢密螺旋体病原体的基因。我们确定了放线杆菌的菌毛基因（fap），以表征其在定植中的作用。该微生物的菌毛菌株在传代到固体培养基上时失去了菌毛。 W发现fap基因的表达在非菌毛菌株中降低(Microbiol.Pathog.1997,23:63-69)。已知蛋白酶对宿主组织发挥细胞毒性作用。我们确定了 B.for ... More sythus 的蛋白酶基因 (prH) 的序列，该基因很难在合成培养基中获得足够的生长以进行表征。该蛋白酶的分子量为47,873，PrtH具有溶血活性(Imfect.Immun.1997, 65:4888-4891)。一些报道表明全身化疗失败是由幽门螺杆菌在口腔定植引起的。我们评估了口腔微生物与 H.pylon 之间的拮抗作用。 H.pylon 与 P.gingivalis 和 F.nucreatum 共聚集。另一方面，一些口腔细菌抑制H.pylon的生长(FEMS Microbial. Lett. 1997, 152: 355-361)。使用野生型菌株和单基因缺陷突变体评价致病性是评价其致病性的最有效方法。我们在 T denticola 中构建了脯氨酰苯丙氨酸特异性蛋白酶（dentilisin）的敲除突变体，并表征了该突变体。该微生物的表面疏水性显着下降，并且突变体形成脓肿的能力下降。此外，我们发现牙本质素与外鞘蛋白的组织有关。这些结果表明牙菌素直接对宿主组织发挥细胞毒性作用(J.Bacteriol.1998, 180:3837-38449)。较少的

项目成果

Toru Saito: "Adherence of oral streptococci to an immobilized antimicrobial agent" Arch. Oral. Biol.42・8. 539-545 (1997)

Toru Saito：“口腔链球菌对固定化抗菌剂的粘附”Arch.42・8（1997）。

Saito, T., Takatsuka, T., Kato, T., Ishihara, K.and Okuda, K.: "Adherence of oral streptococci to an immobilized antimicrobial agent" Archs.oral Biol.42. 539-545 (1997)

Saito, T.、Takatsuka, T.、Kato, T.、Ishihara, K. 和 Okuda, K.：“口腔链球菌对固定抗菌剂的粘附”Archs.oral Biol.42。

Kazuyuki Ishihara: "Oral bacteria inhibit the Helicobacter pylori growth" FFMS Microbiol. Lett.152・2. 355-361 (1997)

Kazuyuki Ishihara：“口腔细菌抑制幽门螺杆菌生长”FFMS Microbiol.152・2（1997）。

Saito,T.: "Adherence of oral Streptococci to an immobilized anrimicrobial agent." Archs.oral Biol.

Saito,T.：“口腔链球菌对固定化抗微生物剂的粘附。”

Kazuyuki Ishihara: "Dentilisin activity affects the organization of the outer sheath of Treponema denticola" J.Bacteriol.180・15. 3837-3844 (1998)

Kazuyuki Ishihara：“牙菌素活性影响密螺旋体外鞘的组织”J.Bacteriol.180・15 3837-3844（1998）。