Analysis of Hellcobacter pylori infection route from oral biological view point.

越来越多的证据表明，幽门螺杆菌的定植与胃溃疡和消化性溃疡的发展有关。本研究检测了口腔微生物对幽门螺杆菌定植的影响，以及对幽门螺杆菌和幽门螺杆菌诱导的免疫球蛋白和免疫球蛋白A的反应性。口腔链球菌、变形链球菌和中间普氏杆菌对幽门螺杆菌的生长有抑制作用。尤其是H.Pylori与中间幽门螺杆菌共同孵育24小时后，未检测到活细胞。这一结果表明幽门螺杆菌可能不会在牙菌斑中生长。此外，我们观察到50%的幽门螺杆菌在暴露于中间螺旋菌或变形链球菌后转变为球状(未培养的形式)(参考文献2)。为了阐明直肠杆菌诱导的免疫应答对幽门螺杆菌感染的作用，我们对胃和十二指肠溃疡患者的唾液和血清中的IgG和IgA滴度进行了测定。唾液中抗幽门螺杆菌和直肠弯曲菌的抗体效价呈正相关。免疫印迹分析表明，兔抗H.Pylori抗体可与直肠肌的蛋白发生反应，兔抗直肠肌抗体可识别H.抗幽门螺杆菌热休克蛋白的抗体与幽门螺杆菌热休克蛋白的分子质量相近(参考文献3)。直肠肌和幽门螺杆菌引起的免疫反应可能与其他系统疾病有关。突眼衣原体HSP抗体水平的升高被认为是动脉粥样硬化的危险因素(Chem et al.2003年，FEMS Innol.Med.Micribiol.36：187-192)。综上所述，抗热休克蛋白抗体诱导的幽门螺杆菌和直肠肌可能与动脉粥样硬化的形成有关(参考文献4)。直肠弯曲菌感染可引起幽门螺杆菌抗体反应，可能影响宿主对幽门螺杆菌的防御。

Increasing evidence has linked colonization by Helicobacterpylori with the development of gastric and peptic ulcer. In this study, a role of oral microorganisms on H. pylori colonization and a reactivity of IgG and IgA elicited by Cainpylorbacter nectus and H.pylori were examined.Streptococcus oralis, Streptococcus mutans, and Prevotella intermedia inhibited the growth of H.pylon. Especially, no viable H.pylori cells were detected after co-incubation of H. pylori and P.intermedia for 24 hours (Ref.1). This result indicated that H. pylori may not grow in dental plaque. In addition, we observed that 50% of H.pylori transferred to coccoid form (uncultivable form) after exposure to P.intermedia or S.mutans (Ref.2).To clarify a role of immune response elicited by C.rectus against H.pylori infection, we evaluated a titer of IgG and IgA in saliva and serum from patients with gastric-and duodenal ulcer. Antibody titers of salivary IgG and IgA against H.pylori and C.rectus correlated each other. Analysis of immunoblotting indicated that rabbit anti-H.pylori antibody reacted to proteins of C.rectus and that rabbit anti-C.rectus antibody recognized H. pylori proteins (Ref.2). A antibody against H.pylori HSP reacted C.rectus protein with similar molecular mass of H.pylori HSP (Ref.3). It is possible that the immunoresponse elicited to C.rectus and H.pylori are associated with other systemic disease. Increase of antibody level against HSP of Chiamydia pneurnoniae was suggested as a risk factor of atherosclerosis (Chem et al. 2003, FEMS Innunol.Med.Micribiol.36: 187-192). Taking these data together, antibody against HSP induced H.pylori and C.rectus possibly associated with atherosclerosis formation (Ref.4).In conclusion, we clarified that the H. pylon attach to oral cavity but does not colonize in it. C.rectus infection elicited antibody react to H. pylori and it might influence on host defense against H.pylori.