Analysis of Hellcobacter pylori infection route from oral biological view point.

越来越多的证据表明幽门螺杆菌的定植与胃溃疡和消化性溃疡的发展有关。本研究检测了口腔微生物对幽门螺杆菌定植的影响，以及幽门螺杆菌和隐门螺杆菌诱导的IgG和IgA的反应性。口腔链球菌、变形链球菌和中间普雷沃氏菌抑制了H.pylon的生长。特别是，在幽门螺杆菌和中间芽孢杆菌共孵育24小时后，没有检测到活的幽门螺杆菌细胞（参考文献1）。提示幽门螺杆菌不可能在牙菌斑中生长。此外，我们观察到50%的幽门螺杆菌在暴露于中间假单胞菌或变形假单胞菌后转移到球虫形式（不可培养形式）（参考文献2）。为了阐明大肠杆菌引起的免疫应答对幽门螺杆菌感染的作用，我们评估了胃和十二指肠溃疡患者唾液和血清中IgG和IgA的滴度。唾液中抗幽门螺杆菌和直梭菌的IgG和IgA抗体滴度具有相关性。免疫印迹分析表明，兔抗h。幽门螺杆菌抗体与直梭菌蛋白和兔抗幽门螺杆菌蛋白反应。rectus抗体识别幽门螺杆菌蛋白（Ref.2）。一种抗H.pylori HSP的抗体反应了与H.pylori HSP分子质量相似的C.rectus蛋白（文献3）。对直梭菌和幽门螺杆菌引起的免疫应答可能与其他全身性疾病有关。chamydia pneurnoniae抗HSP抗体水平升高被认为是动脉粥样硬化的危险因素（Chem et al. 2003; FEMS inunol . med . micribiol）。36: 187 - 192)。综合这些数据，抗HSP抗体诱导的幽门螺杆菌和直梭菌可能与动脉粥样硬化形成有关（参考文献4）。综上所述，我们澄清了牙柱附着于口腔而不是在口腔内定殖。大肠杆菌感染可引起对幽门螺杆菌的抗体反应，并可能影响宿主对幽门螺杆菌的防御。

Increasing evidence has linked colonization by Helicobacterpylori with the development of gastric and peptic ulcer. In this study, a role of oral microorganisms on H. pylori colonization and a reactivity of IgG and IgA elicited by Cainpylorbacter nectus and H.pylori were examined.Streptococcus oralis, Streptococcus mutans, and Prevotella intermedia inhibited the growth of H.pylon. Especially, no viable H.pylori cells were detected after co-incubation of H. pylori and P.intermedia for 24 hours (Ref.1). This result indicated that H. pylori may not grow in dental plaque. In addition, we observed that 50% of H.pylori transferred to coccoid form (uncultivable form) after exposure to P.intermedia or S.mutans (Ref.2).To clarify a role of immune response elicited by C.rectus against H.pylori infection, we evaluated a titer of IgG and IgA in saliva and serum from patients with gastric-and duodenal ulcer. Antibody titers of salivary IgG and IgA against H.pylori and C.rectus correlated each other. Analysis of immunoblotting indicated that rabbit anti-H.pylori antibody reacted to proteins of C.rectus and that rabbit anti-C.rectus antibody recognized H. pylori proteins (Ref.2). A antibody against H.pylori HSP reacted C.rectus protein with similar molecular mass of H.pylori HSP (Ref.3). It is possible that the immunoresponse elicited to C.rectus and H.pylori are associated with other systemic disease. Increase of antibody level against HSP of Chiamydia pneurnoniae was suggested as a risk factor of atherosclerosis (Chem et al. 2003, FEMS Innunol.Med.Micribiol.36: 187-192). Taking these data together, antibody against HSP induced H.pylori and C.rectus possibly associated with atherosclerosis formation (Ref.4).In conclusion, we clarified that the H. pylon attach to oral cavity but does not colonize in it. C.rectus infection elicited antibody react to H. pylori and it might influence on host defense against H.pylori.