Analysis of Hellcobacter pylori infection route from oral biological view point.

越来越多的证据表明，幽门螺杆菌的定植与胃溃疡和消化性溃疡的发生有关。本研究探讨了口腔微生物对H. pylori定殖和由Cainpylorbacternectus和H.pylori引起的IgG和伊加的反应性被检查。口腔链球菌、变形链球菌和中间普氏菌抑制H. pylon的生长。特别是，在与幽门螺杆菌共孵育后未检测到活的幽门螺杆菌细胞。pylori和中间型幽门螺杆菌培养24小时（参考文献1）。结果表明，H.幽门螺杆菌可能不会在牙菌斑中生长。此外，我们观察到，在接触中间型幽门螺杆菌或变形链球菌后，50%的幽门螺杆菌转化为球形（不可培养的形式）（参考文献2）。为了阐明直肌幽门螺杆菌引起的免疫应答对幽门螺杆菌感染的作用，我们评估了胃和十二指肠溃疡患者唾液和血清中IgG和伊加的滴度。唾液中抗幽门螺杆菌和直肌梭菌的IgG和伊加抗体滴度彼此相关。免疫印迹分析表明，兔抗幽门螺杆菌抗体与直肌幽门螺杆菌蛋白反应，兔抗直肌幽门螺杆菌抗体与直肌幽门螺杆菌蛋白反应。pylori蛋白（参考文献2）。抗幽门螺杆菌HSP的抗体与具有相似分子量的幽门螺杆菌HSP的直肌梭菌蛋白反应（参考文献3）。对直肌和幽门螺杆菌引起的免疫应答可能与其他系统性疾病有关。针对肺炎衣原体HSP的抗体水平的增加被认为是动脉粥样硬化的危险因素（Chem等人，2003，FEMS Innunol.Med.Micribiol.36：187-192）。综合这些数据，抗HSP抗体诱导的幽门螺杆菌和直肌梭菌可能与动脉粥样硬化形成有关（参考文献4）。直肌隐孢子虫感染可引起抗幽门螺杆菌的抗体反应。pylori感染，并可能影响宿主对H. pylori的防御。

Increasing evidence has linked colonization by Helicobacterpylori with the development of gastric and peptic ulcer. In this study, a role of oral microorganisms on H. pylori colonization and a reactivity of IgG and IgA elicited by Cainpylorbacter nectus and H.pylori were examined.Streptococcus oralis, Streptococcus mutans, and Prevotella intermedia inhibited the growth of H.pylon. Especially, no viable H.pylori cells were detected after co-incubation of H. pylori and P.intermedia for 24 hours (Ref.1). This result indicated that H. pylori may not grow in dental plaque. In addition, we observed that 50% of H.pylori transferred to coccoid form (uncultivable form) after exposure to P.intermedia or S.mutans (Ref.2).To clarify a role of immune response elicited by C.rectus against H.pylori infection, we evaluated a titer of IgG and IgA in saliva and serum from patients with gastric-and duodenal ulcer. Antibody titers of salivary IgG and IgA against H.pylori and C.rectus correlated each other. Analysis of immunoblotting indicated that rabbit anti-H.pylori antibody reacted to proteins of C.rectus and that rabbit anti-C.rectus antibody recognized H. pylori proteins (Ref.2). A antibody against H.pylori HSP reacted C.rectus protein with similar molecular mass of H.pylori HSP (Ref.3). It is possible that the immunoresponse elicited to C.rectus and H.pylori are associated with other systemic disease. Increase of antibody level against HSP of Chiamydia pneurnoniae was suggested as a risk factor of atherosclerosis (Chem et al. 2003, FEMS Innunol.Med.Micribiol.36: 187-192). Taking these data together, antibody against HSP induced H.pylori and C.rectus possibly associated with atherosclerosis formation (Ref.4).In conclusion, we clarified that the H. pylon attach to oral cavity but does not colonize in it. C.rectus infection elicited antibody react to H. pylori and it might influence on host defense against H.pylori.