EFFECT OF NIFEDIPINE ON INTRACELLULAR SIGNAL TRANSDUCTIONS IN HUMAN GINGIVAL FIBROBLASTS

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Human gingival fibroblasts were established from the patients who had been receiving medication of calcium channel blockers, and undergone the clearance of remaining teeth by the method described previously (Fujii et al., 1994, 1995). After the treatment by nifedipine, the presence of specific phospholylated protein of approximately 30 Kd was confirmed. Increases of small proteins were also observed following the treatments by bradykinin and/or nifedipine. Interleukin-1 and thapsigargin increased intracellular bFGF, but not by nifedipine. The high affinity bFGF-binding site was decreased by 40% through desensitization process. The pre-treatment by nifedipine enhanced intracellular cAMP formation triggered by B-agonist, isoproterenol, in human gingival fibroblasts. In the experiment using gingival fibroblasts from nifedipine reactive patient, the possibility was suggested that nifedipine reactive patient might be susceptible to gingival overgrowth by other calcium channel blockers, such as nicardipine, nisoldipine, diltiazem, verapamil, and phenytoin.