EFFECT OF NIFEDIPINE ON INTRACELLULAR SIGNAL TRANSDUCTIONS IN HUMAN GINGIVAL FIBROBLASTS

硝苯地平对人牙龈成纤维细胞内信号转导的影响

• 批准号: 09671908
• 负责人: FUJII Akira
• 金额: $ 1.92万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671908/
• 关键词: Nifedipine; Intracellular Signaling; Human Gingival Fibroblast; Calcium Channel Blockers; Gingival Overgrowth; 歯肉線維芽細胞; 蛋白リン酸化; bFGF; cAMP; リン酸化蛋白質; IL-1α; ブラジキニン

Human gingival fibroblasts were established from the patients who had been receiving medication of calcium channel blockers, and undergone the clearance of remaining teeth by the method described previously (Fujii et al., 1994, 1995). After the treatment by nifedipine, the presence of specific phospholylated protein of approximately 30 Kd was confirmed. Increases of small proteins were also observed following the treatments by bradykinin and/or nifedipine. Interleukin-1 and thapsigargin increased intracellular bFGF, but not by nifedipine. The high affinity bFGF-binding site was decreased by 40% through desensitization process. The pre-treatment by nifedipine enhanced intracellular cAMP formation triggered by B-agonist, isoproterenol, in human gingival fibroblasts. In the experiment using gingival fibroblasts from nifedipine reactive patient, the possibility was suggested that nifedipine reactive patient might be susceptible to gingival overgrowth by other calcium channel blockers, such as nicardipine, nisoldipine, diltiazem, verapamil, and phenytoin.

从接受钙通道阻滞剂药物治疗的患者建立人牙龈成纤维细胞，并通过先前描述的方法进行剩余牙齿的清除（Fujii等人，1994年，1995年）。用硝苯地平处理后，证实存在约30 Kd的特异性磷酸化蛋白。缓激肽和/或硝苯地平治疗后，也观察到小蛋白的增加。白细胞介素-1和thapsigargin增加细胞内bFGF，但不是硝苯地平。通过脱敏过程，高亲和力bFGF结合位点减少了40%。硝苯地平预处理增强了人牙龈成纤维细胞中由B受体激动剂异丙肾上腺素引发的细胞内cAMP的形成。在使用硝苯地平反应患者的牙龈成纤维细胞的实验中，提示硝苯地平反应患者可能对其他钙通道阻滞剂（如尼卡地平、尼索地平、地尔硫卓、维拉帕米和苯妥英）引起的牙龈过度生长敏感。