EFFECT OF NIFEDIPINE ON INTRACELLULAR SIGNAL TRANSDUCTIONS IN HUMAN GINGIVAL FIBROBLASTS

从接受钙通道阻阻剂治疗的患者中建立人牙龈成纤维细胞，并按照上述方法清除残牙（Fujii et al., 1994,1995）。经硝苯地平治疗后，证实存在约30 Kd的特异性磷酸化蛋白。在缓激肽和/或硝苯地平治疗后，也观察到小蛋白的增加。白细胞介素-1和信号素增加细胞内bFGF，但硝苯地平没有作用。高亲和力的bfgf结合位点通过脱敏过程减少了40%。硝苯地平预处理增强了b激动剂异丙肾上腺素在人牙龈成纤维细胞中引发的细胞内cAMP的形成。在使用硝苯地平反应性患者的牙龈成纤维细胞的实验中，提示硝苯地平反应性患者可能易受其他钙通道阻滞剂（如硝地平、尼索地平、地尔硫卓、维拉帕米和苯妥英）牙龈过度生长的影响。

Human gingival fibroblasts were established from the patients who had been receiving medication of calcium channel blockers, and undergone the clearance of remaining teeth by the method described previously (Fujii et al., 1994, 1995). After the treatment by nifedipine, the presence of specific phospholylated protein of approximately 30 Kd was confirmed. Increases of small proteins were also observed following the treatments by bradykinin and/or nifedipine. Interleukin-1 and thapsigargin increased intracellular bFGF, but not by nifedipine. The high affinity bFGF-binding site was decreased by 40% through desensitization process. The pre-treatment by nifedipine enhanced intracellular cAMP formation triggered by B-agonist, isoproterenol, in human gingival fibroblasts. In the experiment using gingival fibroblasts from nifedipine reactive patient, the possibility was suggested that nifedipine reactive patient might be susceptible to gingival overgrowth by other calcium channel blockers, such as nicardipine, nisoldipine, diltiazem, verapamil, and phenytoin.