Development and application of tooth surface modifier having plaque-controlling ability.

菌斑积聚的原因是由于物理化学相互作用导致微生物附着在固体表面。含氟碳链的硅烷偶联剂具有拒水拒油、防污染、高润滑性、热稳定性和化学稳定性等特点。Yoshino等人合成了两种牙科用硅烷偶联剂，分别命名为1H， 1H, 2H， 2H- henicofluoro十二烷基三异氰基硅烷（10F2S-3I）和1H， 1H, 2H， 2H- henicofluoro十二烷基三甲氧基硅烷（10F2S-3M）。用10F2S-3I或10F2S-3M对树脂复合材料进行表面改性，对细菌在复合材料表面的初始粘附有预防作用。本实验对10F2S-3I在牙釉质表面的表面改性效果进行了评价。牛牙齿标本被切割并顺利完成。将样品分为三组：1)未经处理的对照组，2)表面改性组（MG）：多施30.0mmol/l的10F2S-3M或十二烷基三甲氧基硅烷（10H2S-3M），或3.0mmol/l的10F2S-3I， 3)预处理和表面改性组（PMG）：每个硅烷经磷酸蚀刻后施（PMG）。评估各组标本的表面自由能、钙溶出量、体外变形链球菌的积累和体内菌斑的积累。分别对羟基磷灰石粉（HAP）和牛搪瓷粉（BEP）进行硅烷处理，并采用凝胶渗透色谱法测定其吸附磷维素的量。采用方差分析（ANOVA）和Fisher PLSD进行统计学分析，p<0.05。10F2S-3I和10F2S-3M改性MG和PMG的表面自由能显著低于对照。10F2S-3I和10F2S-3M处理的HAP和BEP上磷光素的吸附量均显著低于对照。MG和PMG在60 min后的钙溶出量显著低于对照组。经10F2S-3I修饰的MG和PMG上积累的变形链球菌总数显著少于对照。经10F2S-3I修饰的MG和PMG在口腔内菌斑积累试验器具上积累的菌斑总量在7 d后显著少于对照组。MG组和PMG组在斑块积累量上无显著差异。本研究结果表明，表面改性剂10F2S-3I可显著降低牙菌斑对牙釉质的粘附。综上所述，使用10F2S-3I可能对维持口腔健康有临床贡献。少

The cause of the plaque accumulation is attributed to the attachment of microorganisms to the solid surface brought about by physicochemical interactions. Silane coupling agents containing fluorocarbon chains have several characteristics such as water and oil repellency, prevention of contamination, high lubricity, and thermal and chemical stability. Yoshino et al. synthesized two dental silane coupling agents named 1H, 1H, 2H, 2H-henicosafluorododecyltriisocyanatosilane (10F2S-3I) and 1H, 1H, 2H, 2H-henicosafluorododecyltrimethoxysilane (10F2S-3M). The surface modification of resin composite with 10F2S-3I or 10F2S-3M has shown a preventive effect on the initial adherence of bacteria to composite surface. In the present experiment, the surface modification efficacy of 10F2S-3I was evaluated on the enamel surface.The bovine teeth specimens were cut and smoothly finished. The specimens were divided into three groups : 1) control group without treatment, 2) surface modified group (MG) : a … More pplied 30.0mmol/l of 10F2S-3M or dodecyltrimethoxysilane (10H2S-3M), or 3.0mmol/l of 10F2S-3I, and 3) pretreated and surface modified group (PMG) : applied each silane after etching with phosphoric acid (PMG). Surface free energy, the amount of calcium dissolution, accumulation of Streptococcus mutans in vitro, and accumulation of plaque in vivo were evaluated in each group of specimens. The hydroxyapatite powder (HAP) and bovine enamel powder (BEP) were also treated with each silane, and amount of adsorbed phosvitin was determined by gel permeation chromatography. The data were analyzed statistically using ANOVA and Fisher PLSD at p<0.05.The surface free energy of MG and PMG modified with 10F2S-3I or 10F2S-3M were significantly lower compared with control. The amount of phosvitin adsorption onto both HAP and BEP treated with 10F2S-3I or 10F2S-3M were significantly less than these of controls. The amount of calcium dissolution from MG and PMG after 60 minutes was significantly less than those of control. The total amount of S.mutans accumulated on MG and PMG modified with 10F2S-3I were significantly less than that of control. The total amounts of plaque accumulated on MG and PMG modified with 10F2S-3I using intra oral plaque accumulation test appliance were significantly less than compared with control after 7 days. There were no significant difference was found between the MG and PMG on the amount of plaque accumulation.From the results obtained in this study, it was suggested that the surface modifier 10F2S-3I significantly reduce plaque adherence to enamel. In conclusion, the use of 10F2S-3I may have clinical contribution to maintenance of oral health. Less