Development and application of tooth surface modifier having plaque-controlling ability.

菌斑堆积的原因是由于物理化学作用带来的微生物在固体表面的附着。含氟碳链的硅烷偶联剂具有拒水拒油、防污染、高润滑性、热稳定性和化学稳定性等特点。Yoshino等人。合成了两种牙科硅烷偶联剂，分别为1H、1H、2H、2H-氟代十二烷基三异氰基硅烷(10F2S-3I)和1H、1H、2H、2H-氟代十二烷基三甲氧基硅烷(10F2S-3M)。用10F2S-3I或10F2S-3M对树脂复合材料进行表面改性，对细菌在复合材料表面的初始黏附有一定的预防作用。在本实验中，评价了10F2S-3I对釉质表面的改性效果。将标本分为3组：1)对照组不处理；2)表面修饰组(MG)：A…10F_2S-3M或十二烷基三甲氧基硅烷(10H_2S-3M)或10F_2S-3I(10F_2S-3I)3.0 mmol/L用量较多；测定每组标本的表面自由能、钙的溶出量、变形链球菌的体外堆积和体内菌斑的堆积。将羟基磷灰石粉(HAP)和牛牙釉质粉(BEP)分别用不同的硅烷处理，用凝胶渗透层析法测定磷高磷蛋白的吸附量。用方差分析和Fisher PLSD对数据进行统计学处理。10F2S-3I和10F2S-3M修饰的MG和PMG的表面自由能显著低于对照组。10F2S-3I和10F2S-3M处理的HAP和BEP对磷高磷蛋白的吸附量均显著低于对照组。60min后，MG和PMG的钙溶出量明显低于对照组。经10F2S-3i修饰的MG和PMG上的变形链球菌总数显著低于对照组。口腔菌斑蓄积试验器观察7天后，10F2S-3I修饰的MG和PMG的菌斑聚集量明显低于对照组。实验结果表明，表面修饰剂10F2S-3i显著降低了菌斑与牙釉质的粘附性。综上所述，10F2S-3i的使用可能对维护口腔健康有一定的临床意义。较少

The cause of the plaque accumulation is attributed to the attachment of microorganisms to the solid surface brought about by physicochemical interactions. Silane coupling agents containing fluorocarbon chains have several characteristics such as water and oil repellency, prevention of contamination, high lubricity, and thermal and chemical stability. Yoshino et al. synthesized two dental silane coupling agents named 1H, 1H, 2H, 2H-henicosafluorododecyltriisocyanatosilane (10F2S-3I) and 1H, 1H, 2H, 2H-henicosafluorododecyltrimethoxysilane (10F2S-3M). The surface modification of resin composite with 10F2S-3I or 10F2S-3M has shown a preventive effect on the initial adherence of bacteria to composite surface. In the present experiment, the surface modification efficacy of 10F2S-3I was evaluated on the enamel surface.The bovine teeth specimens were cut and smoothly finished. The specimens were divided into three groups : 1) control group without treatment, 2) surface modified group (MG) : a … More pplied 30.0mmol/l of 10F2S-3M or dodecyltrimethoxysilane (10H2S-3M), or 3.0mmol/l of 10F2S-3I, and 3) pretreated and surface modified group (PMG) : applied each silane after etching with phosphoric acid (PMG). Surface free energy, the amount of calcium dissolution, accumulation of Streptococcus mutans in vitro, and accumulation of plaque in vivo were evaluated in each group of specimens. The hydroxyapatite powder (HAP) and bovine enamel powder (BEP) were also treated with each silane, and amount of adsorbed phosvitin was determined by gel permeation chromatography. The data were analyzed statistically using ANOVA and Fisher PLSD at p<0.05.The surface free energy of MG and PMG modified with 10F2S-3I or 10F2S-3M were significantly lower compared with control. The amount of phosvitin adsorption onto both HAP and BEP treated with 10F2S-3I or 10F2S-3M were significantly less than these of controls. The amount of calcium dissolution from MG and PMG after 60 minutes was significantly less than those of control. The total amount of S.mutans accumulated on MG and PMG modified with 10F2S-3I were significantly less than that of control. The total amounts of plaque accumulated on MG and PMG modified with 10F2S-3I using intra oral plaque accumulation test appliance were significantly less than compared with control after 7 days. There were no significant difference was found between the MG and PMG on the amount of plaque accumulation.From the results obtained in this study, it was suggested that the surface modifier 10F2S-3I significantly reduce plaque adherence to enamel. In conclusion, the use of 10F2S-3I may have clinical contribution to maintenance of oral health. Less