Development and application of tooth surface modifier having plaque-controlling ability.

菌斑积聚的原因是由于物理化学相互作用导致微生物附着在固体表面。含有氟碳链的硅烷偶联剂具有几个特性，例如防水和防油性、防止污染、高润滑性以及热稳定性和化学稳定性。Yoshino等人合成了两种牙科硅烷偶联剂，分别命名为1H，1H，2 H，2 H-二氟十二烷基三异氰酸酯基硅烷（10 F2 S-3 I）和1H，1H，2 H，2 H-二氟十二烷基三甲氧基硅烷（10 F2 S-3 M）。用10 F2 S-3 I或10 F2 S-3 M对树脂复合材料进行表面改性，对细菌在复合材料表面的初始粘附有一定的预防作用。本实验以牛牙为试件，对10 F2 S-3 I进行表面修饰，观察其对牙釉质表面的修饰效果。将标本分为3组：1）对照组，不处理; 2）表面改性组（MG）：a 关于我们 施加30.0mmol/l的10 F2 S-3 M或十二烷基三甲氧基硅烷（10 H2S-3 M），或3.0mmol/l的10 F2 S-3 I，和3）预处理和表面改性基团（PMG）：在用磷酸（PMG）蚀刻后施加每种硅烷。评价各组标本的表面自由能、钙溶出量、变形链球菌体外蓄积量和菌斑体内蓄积量。羟基磷灰石粉（HAP）和牛牙釉质粉（BEP）也与每一种硅烷处理，并通过凝胶渗透色谱法测定的吸附量。经10 F2 S-3 I或10 F2 S-3 M修饰的MG和PMG的表面自由能均显著低于对照组。经10 F2 S-3 I或10 F2 S-3 M处理的HAP和BEP对黄曲霉素的吸附量均显著低于对照。60 min后MG和PMG的钙溶出量均显著低于对照组。经10 F2 S-3 I修饰的MG和PMG上的变形链球菌总数明显少于对照。经10 F2 S-3 I修饰的MG和PMG在7天后的菌斑累积总量显著低于对照组。结果表明，表面改性剂10 F2 S-3 I能显著降低菌斑在牙釉质上的粘附性。总之，使用10 F2 S-3 I可能对维持口腔健康有临床贡献。少

The cause of the plaque accumulation is attributed to the attachment of microorganisms to the solid surface brought about by physicochemical interactions. Silane coupling agents containing fluorocarbon chains have several characteristics such as water and oil repellency, prevention of contamination, high lubricity, and thermal and chemical stability. Yoshino et al. synthesized two dental silane coupling agents named 1H, 1H, 2H, 2H-henicosafluorododecyltriisocyanatosilane (10F2S-3I) and 1H, 1H, 2H, 2H-henicosafluorododecyltrimethoxysilane (10F2S-3M). The surface modification of resin composite with 10F2S-3I or 10F2S-3M has shown a preventive effect on the initial adherence of bacteria to composite surface. In the present experiment, the surface modification efficacy of 10F2S-3I was evaluated on the enamel surface.The bovine teeth specimens were cut and smoothly finished. The specimens were divided into three groups : 1) control group without treatment, 2) surface modified group (MG) : a … More pplied 30.0mmol/l of 10F2S-3M or dodecyltrimethoxysilane (10H2S-3M), or 3.0mmol/l of 10F2S-3I, and 3) pretreated and surface modified group (PMG) : applied each silane after etching with phosphoric acid (PMG). Surface free energy, the amount of calcium dissolution, accumulation of Streptococcus mutans in vitro, and accumulation of plaque in vivo were evaluated in each group of specimens. The hydroxyapatite powder (HAP) and bovine enamel powder (BEP) were also treated with each silane, and amount of adsorbed phosvitin was determined by gel permeation chromatography. The data were analyzed statistically using ANOVA and Fisher PLSD at p<0.05.The surface free energy of MG and PMG modified with 10F2S-3I or 10F2S-3M were significantly lower compared with control. The amount of phosvitin adsorption onto both HAP and BEP treated with 10F2S-3I or 10F2S-3M were significantly less than these of controls. The amount of calcium dissolution from MG and PMG after 60 minutes was significantly less than those of control. The total amount of S.mutans accumulated on MG and PMG modified with 10F2S-3I were significantly less than that of control. The total amounts of plaque accumulated on MG and PMG modified with 10F2S-3I using intra oral plaque accumulation test appliance were significantly less than compared with control after 7 days. There were no significant difference was found between the MG and PMG on the amount of plaque accumulation.From the results obtained in this study, it was suggested that the surface modifier 10F2S-3I significantly reduce plaque adherence to enamel. In conclusion, the use of 10F2S-3I may have clinical contribution to maintenance of oral health. Less