Development and application of water soluble surfactant which inhibit the adherence of plaque onto tooth surface.

我们认为，降低牙齿表面的表面自由能可能有助于预防龋齿和牙周病。本文合成了一种新型的杂化表面活性剂，命名为sodiuml-[磷酸（4-庚-癸基辛基）-1-己基苯酯（F8H5PPhNa），sodium1-[[4-（十七氟辛基）苯基]-1-丁基苯基磷酸酯（F8 H3 PPhNa）、1H，1H，2 H，2 H-全氟己基苯基磷酸（F4）、1H，1H，2 H，2 H-全氟辛基苯基磷酸（F6）和1H，1H，2 H，2 H-全氟癸基苯基磷酸（F8），分子中含有碳氟链和烃链。本研究采用新型复合表面活性剂F_8H_5PPhNa、F_8H_3PPhNa和20 mmol/l的F_4、F_6、F_8/5%乙醇溶液对牛牙釉质表面进行表面改性，研究了复合表面活性剂处理后牙釉质表面的表面自由能和耐酸性能。表面自由能的计算使用滴法。为了评价改性表面的防水性，将釉质样品在37℃的水中储存1、3、7、14、21、28、60和90天。用原子吸收光谱法测定试样表面Ca^2+含量，用扫描电镜观察试样表面形貌，结果表明，经表面活性剂改性后的试样表面自由能显著低于对照组（p<0.05）。然而，在水中储存3天后，所有组的表面自由能均增加。改良组细胞内Ca^<2+>溶出量低于对照组（p<0.05）。F8修饰组是对照组的四分之一，并且是组中最低的（p<0.01）。结果表明，F8表面活性剂具有良好的抗牙菌斑作用，可有效地抑制牙釉质表面的牙菌斑堆积。

We have considered that decreasing the surface free energy of tooth surface may contribute to prevention of caries and periodontal disease. We have synthesized the novel hybrid surfactants named sodiuml-[(4hepta- decafluorooct1)-1-hexyl-phenyl phosphate (F8H5PPhNa), sodium1-[(4heptadecafluorooctyl)phenyl]-1-butylphenyl phosphate (F8H3PPhNa), 1H,1H,2H,2H-perfluorohexylphenylphosphoric acid (F4), 1H,1H,2H,2H-perfluorooctyl phenyl phosphoric acid (F6), and 1H,1H,2H,2H-perfluorodecyl phenyl phosphoric acid (F8) containing a fluorocarbon chain and hydrocarbon chain in a molecule. The aim of this study was to investigate the surface free energy and the acid resistance of the enamel surface treated with novel hybrid surfactants.Smoothly finished bovine enamel surface was modified with F8H5PPhNa, F8H3PPhNa prepared to 1mmol/l with distilled water, and with a 20mmol/l of F4, F6 or F8/5% ethanol. The surface free energies were computed using the Drop Method. In order to evaluate water-proof of the modified surface, the enamel specimens were stored in water at 37℃ for 1, 3, 7, 14, 21, 28, 60, and 90 days. The specimens were immersed in a 0.1 mol/l of sodium acetate buffer of pH4.0 for 30min and the amounts of Ca^<2+> was measured by atomic absorption analysis.The surfaces of specimens were observed by SEM.The surface free energy of modified by these surfactants were significantly lower than the control (p<0.05). However, the surface free energies of all groups were increased after 3-day storage in water. The amounts of Ca^<2+> dissolved from modified groups were less than control (p<0.05). The F8 modified group was a quarter of that of the control and the lowest of the groups (p<0.01). The F8 modified enamel surface also showed less decalcification compared with that of the control by SEM observation.From these results, it was suggested that the novel hybrid and aqueous surfactants i.e.F8 is promising to prevent plaque accumulation on the enamel surface.