Circannual Rhythms in the Effect of Neuroleptics

1)为了阐明8-OH-DPAT是否存在昼夜节律和昼夜节律，在不同季节、一天中的不同时间给雄性Wistar大鼠皮下注射8-OH-DPAT，通过记录总共90 mm的前爪踩踏和编织头部的总频率来量化5-羟色胺综合征。每只大鼠只被使用一次。实验每隔3个月重复5次。2)在被测的每个月中，前爪践踏和头部编织都表现出显著的昼夜节律。3)在所有被测时间点上，前爪践踏和头部编织都表现出显著的年节律。昼夜节律的位相表现出一年的周期变化，年节律的位相表现出一天的周期变化。在相同温度和相同光暗期的恒定环境条件下世代饲养的动物，对8-OH-DPAT反应的周年节律得到证实。考虑到这一点，我们强烈地认为这不是一种基于对光周期或环境温度季节变化的直接反应的现象，而是一种内源性的昼夜节律。4)结果表明，药物的反应性既存在昼夜节律，又存在昼夜节律。5)接下来，通过观察体温过低(5-HIIA受体功能的另一个指标)来检查对8-OH-DPAT的反应性的节律。评估低温反应的参数显示出明显的昼夜节律和昼夜节律。6)通过观察大鼠的镇静作用来检验氟哌啶醇的昼夜节律。

1) In order to elucidate whether circadian and circannual rhythms exist in the effect of 8-OH-DPAT,8-OH-DPAT was administered subcutaneously to male Wistar rats at different hours of the day in different seasons, and the 5-HT syndrome was quantified by recording the total frequency of forepaw treading and head weaving for a total of 90 mm. Each rat was used only once. The experiments were repeated five time at three-month intervals.2) Both forepaw treading and head weaving exhibited a significant circadian rhythm in every month examined.3) Both forepaw treading and head weaving exhibited a significant annual rhythm at all time points tested. The phases of the circadian rhythm exhibit one year period variations while those of the annual rhythm exhibit one day period variations. The annual rhythm in the responsiveness to 8-OH-DPAT was confirmed in animals that were kept under constant environmental conditions of the same temperature and the same light-dark period through successive generations. Taking this into account, it is strongly suggested that this is not a phenomenon based on direct reaction to either the photoperiodicity or seasonal change in the environmental temperature, but this is an endogenous circannual rhythm.4) The results suggest that both circadian and circannual rhythms exist in the responsiveness to the drug.5) Next, the rhythm in the responsiveness to 8-OH-DPAT was examined by observing hypothermia, another index of the 5-HIIA receptor function. The parameters of assessing the hypothermic response displayed significant circadian and circannual rhythms.6) Circannual rhythm in the effect of haloperidol is being examined by observing sedative effects in rats.