Evolution of the WOX gene family and the stem cell niche

WOX基因家族和干细胞生态位的进化

• 批准号: 82326851
• 负责人: Professor Dr. Wolfgang Werr
• 金额: --
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/82326851?language=en
• 关键词: Evolution; WOX; gene; family; stem

Robust phylogenetic reconstructions imply a monophyletic origin of the WOX homeobox gene family in green algae and successive gene amplifications from basal land plants over gymnosperms to basal angiosperms. Members of the ancient WOX13 lineage are still present in extant higher plant species, but their genealogy and functional relation to modern family members is unclear. The modern WUSCHEL and WOX5 genes promote stem cell fate in the shoot or root meristem of Arabidopsis thaliana and represent an angiosperm-specific duplication from a common gymnosperm WUS/WOX5 ancestor. This project aims to analyse the origin of the WUS or WOX5 functions in the stem cell niches of seed plant and their relation to the ancient WOX13 gene lineage. It uses homologous recombination in Physcomitrella patens, available Arabidopsis T-DNA insertion lines and asks for functional equivalence of WUS and WOX5 orthologues in basal angiosperms (Nymphaea jamesoniana) or their Ginkgo, Gnetum or Pinus ancestors. Evolutionary information highlighted by conserved polypeptide motifs in WUS or WOX5 orthologues from basal angiosperms or gymnosperm ancestors will guide wus-1 rescue experiments in Arabidopsis thaliana.

稳健的系统发育重建暗示了绿色藻类中WOX同源盒基因家族的单系起源，以及从裸子植物到基底被子植物的连续基因扩增。古老的WOX 13家族成员仍然存在于现存的高等植物物种中，但它们的谱系和与现代家族成员的功能关系尚不清楚。现代WUSCHEL和WOX 5基因促进拟南芥茎或根分生组织中的干细胞命运，并且代表来自共同裸子植物WUS/WOX 5祖先的被子植物特异性复制。该项目旨在分析种子植物干细胞小生境中WUS或WOX 5功能的起源及其与古老WOX 13基因谱系的关系。它在小立碗藓（Physcomitrella patens）、可用的拟南芥T-DNA插入系中使用同源重组，并要求在基部被子植物（睡莲（Nymphaea jamesoniana））或它们的银杏、买麻藤或松树祖先中WUS和WOX 5直系同源物的功能等同性。进化信息突出的保守多肽基序在WUS或WOX 5直系同源物从基础被子植物或裸子植物的祖先将指导wus-1在拟南芥的救援实验。