The Arabidopsis shoot apical meristem tool kit: a unifying set of cell type specific driver and effector lines for functional analysis of cellular identities in the SAM

拟南芥芽顶端分生组织工具包：一套统一的细胞类型特异性驱动器和效应器系，用于 SAM 中细胞身份的功能分析

• 批准号: 217062504
• 负责人: Professor Dr. Wolfgang Werr
• 金额: --
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/217062504?language=en
• 关键词: Arabidopsis; shoot; apical; meristem; tool

Genetic and molecular studies have identified numerous genes in Arabidopsis thaliana, which contribute essential components for shoot apical meristem (SAM) function, and have streamlined our conceptual view on meristem organisation, with respect to layering and zonation. With the DORNRÖSCHEN-LIKE transcription factor, we have identified a novel gene function, that marks lateral organ founder cells and expression precedes auxin maxima at the SAM periphery. This project aims to combine well-characterised, SAM-specific promoters into one set of driver lines that stably (LhG4) or inducibly (LhG4GR) address different cell types or compartments within the SAM or incipient primordia. These driver lines are characterised in combinations with different fluorophore reporters, which can concomitantly be analysed with existing stable promoter::GFP lines; immediate results will therefore be comparative promoter activities and their dynamics within the SAM. However, bona fide driver lines can then be used to predictably express transgenes in the shoot apex and be freely combined with many kinds of effector constructs. The resulting toolbox will substantially improve the repertoire of analytic methods in the shoot apex and provide a new community resource with which to access regulatory networks in the SAM. With respect to new technology developments, we will test whether cell type-specific CRE/loxP-dependent activation of the GUS reporter or inducible cell ablation with the diphtheria A toxin cell death reporter can identify clonal lineages within the SAM and elucidate on the contribution of individual cell types.

遗传和分子研究已经确定了许多基因在拟南芥，这有助于茎顶端分生组织（SAM）功能的重要组成部分，并简化了我们的概念观点分生组织，分层和分区。与DORNRÖSCHEN样转录因子，我们已经确定了一种新的基因功能，标志着侧器官创始人细胞和表达之前，生长素极大的SAM周边。该项目旨在将联合收割机充分表征的SAM特异性启动子结合到一组驱动系中，该驱动系稳定地（LhG 4）或诱导地（LhG 4GR）针对SAM或初始原基内的不同细胞类型或隔室。这些驱动线的特征在于与不同的荧光团报告，这可以伴随着与现有的稳定的启动子：：GFP线进行分析的组合，因此，直接的结果将是比较启动子的活性和它们的动态内的SAM。然而，真正的驱动线，然后可以用于可预测地表达转基因在茎尖和自由组合与许多种效应构建体。由此产生的工具箱将大大提高在拍摄顶点的分析方法的剧目，并提供了一个新的社区资源与SAM中访问监管网络。关于新技术的发展，我们将测试是否细胞类型特异性CRE/loxP依赖性激活的GUS报告或诱导细胞消融与白喉A毒素细胞死亡报告可以确定克隆谱系内的SAM和阐明的贡献个别细胞类型。