Appendix and ulcerative colitis

阑尾和溃疡性结肠炎

• 批准号: 09670543
• 负责人: OKAZAKI Kazuichi
• 金额: $ 1.86万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670543/
• 关键词: IBD; Appendix; MALT

The mouse has the lymphoid organs like Peyer's patches in the cecum, colon and rectum. However, it is unknown whether they have their own independent or specific functions. Injection of anti-IL-7 receptor antibody into pregnant mice can induce Peyer's patch deficient mice (PPD-mice), and we studied the immunological findings of these mice.Anti-IL-7 receptor antibody was injected to normal BALB/c mice on the 14th day of pregnancy. Whole-mount immunohistochemical staining showed that the neonatal mice have no Peter's patch but have colonic and rectal patches. They are orally or rectally immunized once a week with 2mg of KLH, or KLH + 10μg of cholera toxin 3 times. Normal BALB/c mice immunized with the same dose were served as control. In some mice, appendectomy was performed to remove the cecal patch: Serum levels of immunogloblins (Igs) and secretary IgA in the small intestinal fluids were measured by ELISA system. Intra-epithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) were analyzed by flow cytometry.Levels of serum Igs and secretory IgA specific for KLH in the rectally immunized PPD-mice were higher than those in the orally immunized PPD-mice and control mice. The PPD mice without appendix have higher levels of the serum Igs specific for KLH than PPD-mice or control mice. In orally immunized PPD mice, appandectomy induced significant increase in the ratio of serum IgG1/IgG2a specific for KLH. Flow cytometry revealed that B1cells (CD5+,B220 low,s-IgMhigh) are not identified in LPL.Rectal immunization induce potent immune responses compared with the oral immunization in the mice. Colonic and Rectal patches seem to be independent from intestinal Peyer's patches in the mucosal immune system. Moreover, the appendix may modulate systemic immune response.

小鼠的盲肠、结肠和直肠中都有像佩耶氏斑这样的淋巴器官。然而，它们是否有自己独立的或特定的功能是未知的。在妊娠小鼠体内注射抗il -7受体抗体可诱导Peyer's patch deficient mice (PPD-mice)，并对其免疫学结果进行了研究。在妊娠第14天给正常BALB/c小鼠注射抗il -7受体抗体。全载免疫组化染色显示，新生小鼠无Peter’s斑块，但有结肠和直肠斑块。每周口服或直肠接种1次KLH 2mg，或KLH + 10μg霍乱毒素3次。以免疫相同剂量的正常BALB/c小鼠为对照。部分小鼠行阑尾切除术，取盲肠补片，ELISA法测定小鼠小肠液中免疫球蛋白（igg）和分泌IgA的血清水平。流式细胞术检测上皮内淋巴细胞（IELs）和固有层淋巴细胞（LPLs）。直肠免疫ppd小鼠血清igg和分泌的KLH特异性IgA水平高于口服免疫ppd小鼠和对照小鼠。没有阑尾的PPD小鼠血清中KLH特异性igg水平高于PPD小鼠或对照小鼠。在口服免疫PPD小鼠中，阑尾切除术导致KLH特异性血清IgG1/IgG2a比例显著升高。流式细胞术显示，LPL中未发现b1细胞（CD5+、B220低、s-IgMhigh）。与口服免疫相比，直肠免疫可诱导小鼠产生较强的免疫应答。在粘膜免疫系统中，结肠和直肠斑块似乎独立于肠道Peyer's斑块。此外，阑尾可能调节全身免疫反应。

项目成果

Okazaki K,et al.: "Multiple pancreatic masses associated with autoimmunity"Am J Gastroenterol. 93. 99-102 (1998)

Okazaki K 等人：“与自身免疫相关的多发性胰腺肿块”Am J Gastroenterol。

Okazaki K,et al.: "Open chest darinage for the postoperative empyema in Boerhaave's syndrome"Dig Dis Sci. 43. 1518-1520 (1998)

Okazaki K 等人：“开胸引流治疗 Boerhaave 综合征术后脓胸”Dig Dis Sci。

Okazaki K,et al.: "Vector manometry of the sphincter of Oddi. Efitorials -A new parameter of the pancreato-biliary physiology-"J Gastroenterol. 33(6). 923-924 (1998)

冈崎 K 等人：“Oddi 括约肌的矢量测压法。Efitorials -胰胆生理学的新参数 -”J Gastroenterol。

Okazaki K,: "Lack of association of anisakiasis and gastric inflammatory fibroid polyps." Gastrointst Endose. 46. 92-93 (1997)

Okazaki K：“异尖线虫病和胃炎性纤维性息肉之间缺乏关联。”

Okazaki K,et al.: "Efficacy of total colonoscopy with a transparent cap in comparison with colonoscopy without the cap"Endoscopy. 30. 444-447 (1998)

Okazaki K 等人：“带透明帽的全结肠镜检查与不带帽的结肠镜检查的功效相比”内窥镜检查。