Appendix and ulcerative colitis

小鼠在盲肠、结肠和直肠中具有淋巴器官，如派尔集合淋巴结。但是，它们是否有自己独立或特定的功能尚不清楚。将抗IL-7受体抗体注射到妊娠小鼠体内，可诱导出派伊尔斑缺陷小鼠（Peyer 'spatch deficiency mice，PPD-mice），并观察了PPD-mice的免疫学变化。免疫组织化学染色显示，新生小鼠无彼得斑，但有结肠和直肠斑。每周口服或直肠免疫1次，每次2 mg KLH，或KLH + 10μg霍乱毒素3次。正常BALB/c小鼠以相同剂量免疫作为对照。在一些小鼠中，进行阑尾切除术以去除盲肠片：用ELISA系统测定血清免疫球蛋白（Ig）和小肠液中的分泌型伊加水平。经直肠免疫的PPD小鼠血清中KLH特异性Ig和分泌伊加水平均高于经口免疫的PPD小鼠和对照组小鼠。无阑尾的PPD小鼠血清KLH特异性Ig水平高于PPD小鼠或对照小鼠。在口服免疫的PPD小鼠中，阑尾切除诱导KLH特异性的血清IgG 1/IgG 2a比率显著增加。流式细胞仪检测显示，LPL中未检测到B1细胞（CD 5+，B220低，s-IgM高）。在粘膜免疫系统中，结肠和直肠斑似乎独立于肠派尔斑。此外，阑尾可以调节全身免疫反应。

The mouse has the lymphoid organs like Peyer's patches in the cecum, colon and rectum. However, it is unknown whether they have their own independent or specific functions. Injection of anti-IL-7 receptor antibody into pregnant mice can induce Peyer's patch deficient mice (PPD-mice), and we studied the immunological findings of these mice.Anti-IL-7 receptor antibody was injected to normal BALB/c mice on the 14th day of pregnancy. Whole-mount immunohistochemical staining showed that the neonatal mice have no Peter's patch but have colonic and rectal patches. They are orally or rectally immunized once a week with 2mg of KLH, or KLH + 10μg of cholera toxin 3 times. Normal BALB/c mice immunized with the same dose were served as control. In some mice, appendectomy was performed to remove the cecal patch: Serum levels of immunogloblins (Igs) and secretary IgA in the small intestinal fluids were measured by ELISA system. Intra-epithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) were analyzed by flow cytometry.Levels of serum Igs and secretory IgA specific for KLH in the rectally immunized PPD-mice were higher than those in the orally immunized PPD-mice and control mice. The PPD mice without appendix have higher levels of the serum Igs specific for KLH than PPD-mice or control mice. In orally immunized PPD mice, appandectomy induced significant increase in the ratio of serum IgG1/IgG2a specific for KLH. Flow cytometry revealed that B1cells (CD5+,B220 low,s-IgMhigh) are not identified in LPL.Rectal immunization induce potent immune responses compared with the oral immunization in the mice. Colonic and Rectal patches seem to be independent from intestinal Peyer's patches in the mucosal immune system. Moreover, the appendix may modulate systemic immune response.