Cell and molecular Biological Study of 60Da mucin molecules.

60Da 粘蛋白分子的细胞和分子生物学研究。

• 批准号: 05670486
• 负责人: OKAZAKI Kazuichi
• 金额: $ 1.34万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670486/
• 关键词: cancer; mucin; stomach

Abstract : We have previously studied the biosynthesis and secretion of mucin in the normal human stomach and reported that the tetramer of the 60-kDa subunit of mucin core protein was synthesized and highly glycosylated, and that only high molecular weight mucin was secreted. In this study, we investigated the mucin-related products of a gastric cancer cell line (Hs746T). Analysis of intracellular and extracellular products immunoprecipitated with an anti-apomucin monoclonal antibody revealed that a ll0-kDa protein, the dimer of the 55-kDa subunit, was synthesized and secreted. Tunicamycin treatment inhibited the secretion of the ll0-kDa protein. These findings suggest that N-glycosylation may be involved in the secretory mechanism of the mucin-related product.charides, and some of these antigens have been used as tumor markers.^<3-7> Low molecular weight mucin-related products, as well as high molecular weight ones, can be detected in the serum of cancer patients.^<H-11> However, very little is known about the intracellular transport and secretory mechanisms of mucin-related products. In the present study. we examined this mechanism in a human gastric carcinoma cell line (Hs746T) and compared it to that in the normal gastric mucosa. After pulse-labeling with [^<35>S] methionine and chase incubation, we analyzed the intracellular and extracellular products with an anti-carboxyl terminal of the apomucin monoclonal anti-body.

摘要：我们之前研究了正常人胃中粘蛋白的生物合成和分泌，报道了粘蛋白核心蛋白60-kDa亚基的四聚体被合成并高度糖基化，并且仅分泌高分子量粘蛋白。在这项研究中，我们研究了胃癌细胞系（Hs746T）的粘蛋白相关产物。用抗阿普粘蛋白单克隆抗体免疫沉淀的细胞内和细胞外产物的分析表明，合成并分泌了110-kDa蛋白，即55-kDa亚基的二聚体。衣霉素处理抑制了110-kDa蛋白的分泌。这些发现表明，N-糖基化可能参与了粘蛋白相关产物的分泌机制。charides，其中一些抗原已被用作肿瘤标志物。^<3-7>在癌症患者的血清中可以检测到低分子量和高分子量的粘蛋白相关产物。^<H-11>然而，人们对粘蛋白相关产物的细胞内转运和分泌机制知之甚少。 粘蛋白相关产品。在目前的研究中。我们在人胃癌细胞系（Hs746T）中检查了这种机制，并将其与正常胃粘膜中的机制进行了比较。在用[^ 35 S]甲硫氨酸脉冲标记并追踪孵育后，我们用apomucin单克隆抗体的抗羧基末端分析了细胞内和细胞外产物。

项目成果

Okazaki K, Sano S, Suenaga K, Morita M, Yamamoto Y.: "Biosynthesis and Secretion of mucin in the normal and gastric cancer patients." Gastroenterology. 105. A151-151 (1994)

Okazaki K、Sano S、Suenaga K、Morita M、Yamamoto Y.：“正常人和胃癌患者粘蛋白的生物合成和分泌。”

岡崎和一他: "消化性潰瘍研究" 伊東進監修:, 97 (1993)

Kazuichi Okazaki 等：“消化性溃疡研究”，由 Susumu Ito 监督：, 97 (1993)

Okazaki K,Sano S,Yamamoto Y.: "Roles of N-glycans in the transport of mucin in gastric cancer cells." Gastroenterology. 104. A164 (1993)

Okazaki K，Sano S，Yamamoto Y.：“N-聚糖在胃癌细胞粘蛋白运输中的作用。”

Sano S, Okazaki K, Yamamoto Y.: "Biosynthesis and Secretion of mucin in the human stomach." Journal of Gastroenterology. 29. 685-694 (1994)

Sano S、Okazaki K、Yamamoto Y.：“人胃中粘蛋白的生物合成和分泌。”

Okazaki K,Sano S,Miyata M,Yamamoto Y.: "Effects of short-term administration of dexamethasone on gastrin gene expression." Digestion. 54. 130-34 (1993)

Okazaki K，Sano S，Miyata M，Yamamoto Y.：“短期服用地塞米松对胃泌素基因表达的影响。”