Development of a novel method for labeling of sugar chain and their application for analysis of saliva-specific ABH epitopes

为了简化糖链的分析，研究了一种新的糖链标记方法，该方法可用于糖链的高效液相色谱分析、酶联免疫吸附分析和薄层免疫印迹分析。以氨基苯甲酸和脂肪醇(C8-C16)为原料合成了芳香族氨基酸酯类化合物(氨基-C&46)。利用新开发的标记试剂对从唾液粘蛋白中获得的ABO血型活性糖链进行了分析。虽然我们不能获得能被高效液相分析的、具有足够疏水性结合固相用于免疫学分析的新型标记试剂，但我们已经成功地获得了许多有用的针对ABO血型的单抗，并建立了一种选择性生产抗糖蛋白部分和多肽部分的单抗的方法。

A novel method for labeling of sugar chain, which can be used for analysis of sugar chain by HPLC, ELISA and TLC immunoblotting was investigated for simplification of analysis of sugar chains. To prepare reagents which have sufficient hydrophobicity to bind to solid-phases, ELISA plates or blotting membranes, aromatic aminoester compounds (amino-C&46) were synthesized from aminobenzoic acid and aliphatic alcohols (C8-C 16). Analysis of ABO blood group active sugar chains obtained from saliva mucin was performe4 by using the newly developed labeling reagents. However, we could not obtain satisfactory results.Although we could not obtain novel labeling reagents which were analyzable by HPLC and have sufficient hydrophobicity to bind solidphases for immunological analyses, we have succeeded to obtain many useful monoclonal antibodies specific ABO blood group and have established a methods for selective production of monoclonal antibodies to sugar moieties and peptide moieties of glycoproteins.