Development of a novel method for labeling of sugar chain and their application for analysis of saliva-specific ABH epitopes

糖链标记新方法的开发及其在唾液特异性 ABH 表位分析中的应用

• 批准号: 09670449
• 负责人: KIMURA Akihiko
• 金额: $ 1.54万
• 依托单位: Wakayama Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670449/
• 关键词: sugar chains; fluorescence labeling; HPLC; ELISA; TLC

A novel method for labeling of sugar chain, which can be used for analysis of sugar chain by HPLC, ELISA and TLC immunoblotting was investigated for simplification of analysis of sugar chains. To prepare reagents which have sufficient hydrophobicity to bind to solid-phases, ELISA plates or blotting membranes, aromatic aminoester compounds (amino-C&46) were synthesized from aminobenzoic acid and aliphatic alcohols (C8-C 16). Analysis of ABO blood group active sugar chains obtained from saliva mucin was performe4 by using the newly developed labeling reagents. However, we could not obtain satisfactory results.Although we could not obtain novel labeling reagents which were analyzable by HPLC and have sufficient hydrophobicity to bind solidphases for immunological analyses, we have succeeded to obtain many useful monoclonal antibodies specific ABO blood group and have established a methods for selective production of monoclonal antibodies to sugar moieties and peptide moieties of glycoproteins.

为简化糖链分析，研究了一种新的糖链标记方法，可用于HPLC、ELISA和TLC免疫印迹分析。为了制备具有足够疏水性以结合到固相、ELISA板或印迹膜的试剂，由氨基苯甲酸和脂肪醇（C8-C16）合成芳香族氨基酯化合物（氨基-C46）。用新研制的标记试剂对从唾液粘蛋白中提取的ABO血型活性糖链进行了分析。尽管我们还没有获得新的标记试剂，这些标记试剂既可以用HPLC分析，又具有足够的疏水性，可以结合固相用于免疫分析，但我们已经成功地获得了许多有用的ABO血型特异性单克隆抗体，并建立了一种选择性生产糖蛋白糖部分和肽部分单克隆抗体的方法。

项目成果

Kimura A.: "Monoclonal antibodies to ABO blood group antigens specific for body fluids" ADVANCES IN LEGAL MEDICINE. 3. 599-602 (1997)

Kimura A.：“体液特异性 ABO 血型抗原的单克隆抗体”法律医学的进步。

Kimura A.: "Differential production of monoclonal antibodies to carbohydrate moiety or peptides moiety of glycoproteins by different routes of immunization" HYBRIDOMA. 17-3. 245-250 (1998)

Kimura A.：“通过不同的免疫途径不同地生产针对糖蛋白的碳水化合物部分或肽部分的单克隆抗体”HYBRIDOMA。

Kimura Akihiko: "Differential production of monoclonal antibodies to carbohydrate moiety or peptides moiety of glycoproteins by different routes of immunization" HYBRIDOMA. 17・3. 245-250 (1998)

Kimura Akihiko：“通过不同的免疫途径不同地生产针对糖蛋白的碳水化合物部分或肽部分的单克隆抗体”17・3（1998）。

Kimura Akihiko: "Monoclonal antibodies to ABO blood group antigens specific for body fluids" ADVANCES IN LEGAL MEDICINE. 3. 599-602 (1997)

Kimura Akihiko：“体液特异性 ABO 血型抗原的单克隆抗体”法律医学的进步。