Development of novel diagnostic methods for anaphylactic shock based on gene expression

基于基因表达的过敏性休克新诊断方法的开发

• 批准号: 24659339
• 负责人: KIMURA Akihiko
• 金额: $ 2.25万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659339/
• 关键词: アナフィラキシーショック; 法医診断; 急性死; ショック; アナフィラキシー

Specifying an anaphylactic shock as the cause of death only based on the autopsy findings often encounters difficulties. So, in this research, we tried development of the novel diagnostic method for anaphylactic shock using the mouse model. Anaphylactic shock was induced by challenge of penicillin V as an antigen, and total RNA was extracted from the various organs of mice died. The extracted RNA was subjected to real-time RT-PCR for analyses of the gene expression of cytokines, chemokines, the molecules for immune regulation, growth factors, transcription factors and signal transducers to identify the changes in specific gene expression anaphylactic shock. However, at present, we did not find the changes of gene expression specific for anaphylactic shock.

仅根据尸检结果确定过敏性休克为死亡原因往往会遇到困难。因此，在本研究中，我们尝试开发一种利用小鼠模型诊断过敏性休克的新方法。以青霉素V为抗原攻毒致过敏性休克，从死亡小鼠各脏器中提取总RNA。提取的RNA进行实时RT-PCR分析细胞因子、趋化因子、免疫调节分子、生长因子、转录因子和信号转导的基因表达，以确定过敏性休克特异性基因表达的变化。然而，目前我们没有发现过敏性休克特异性基因表达的变化。

项目成果

皮膚組織におけるautophagyのcircadian rhythmと創傷治癒

皮肤组织自噬和伤口愈合的昼夜节律

• 发表时间: 2013
• 作者: Kimura A;Ishida Y;Nosaka M;Kuninaka Y;Mukaida N;Kondo T.;近藤稔和,木村章彦,石田裕子,野坂みずほ,國中由美,川口真理子
• 通讯作者: 近藤稔和,木村章彦,石田裕子,野坂みずほ,國中由美,川口真理子

圧負荷による代償性心肥大の分子メカニズム-IFN-の役割について

压力超负荷导致代偿性心脏肥大的分子机制——IFN-γ的作用

• 发表时间: 2013
• 作者: Kimura A;Ishida Y;Nosaka M;Kuninaka Y;Mukaida N;Kondo T.;近藤稔和,木村章彦,石田裕子,野坂みずほ,國中由美,川口真理子;木村章彦,野坂みずほ,向田直史,近藤稔和
• 通讯作者: 木村章彦,野坂みずほ,向田直史,近藤稔和

Immunohistochemical detection of intrathrombotic macrophage-derived cytokines and its application to thrombus age estimation in murine deep vein thrombosis model

• DOI: 10.1007/s00414-013-0873-0
• 发表时间: 2013-05
• 期刊: International Journal of Legal Medicine
• 影响因子: 2.1
• 作者: M. Nosaka;Y. Ishida;A. Kimura;T. Kondo

IFN- gamma plays a protective role in pressure overload-induced cardiac hypertrophy through PI3K/Akt signaling activation

IFN-γ 通过 PI3K/Akt 信号激活在压力超负荷引起的心脏肥大中发挥保护作用

• 发表时间: 2012
• 作者: Yutaka Oomura;Shuji Aou;Kohji Fukunaga;Kazuo Sasaki;Kimura Akihiko
• 通讯作者: Kimura Akihiko

Lack of IFN-gamma exergarates ANG II-induced aortic aneurysm through reduction of autophagy

缺乏 IFN-γ 通过减少自噬加剧 ANG II 诱导的主动脉瘤

• 发表时间: 2013
• 作者: Kimura A;Ishida Y;Nosaka M;Kuninaka Y;Mukaida N;Kondo T.
• 通讯作者: Kondo T.