Development of growth and flowering control of cut flower by DIF

对一些一年生花卉进行了试验。举几个例子，常用的品种有：Mathiola incana（普通砧木）、Dianthus hybrida（杂交粉红）、Dianthus caryophyllus（石竹）、antirium majus（金鱼龙）、Petunia hybrida（矮牵牛）、Viola X wittrockiana（三色堇）幼苗。种子在滤纸上萌发，在连续保持正（+5）、零(0)、负（-5）DIF的生长室中萌发。（虽然平均温度是相同的，但DIF被定义为昼夜温度之间的数学差）。一个月后测量下胚轴的长度。DIF对下胚轴长度的影响不明显。培养皿中的对照苗不均匀，因此这种方法被认为是不合适的。第二年，提供了液体施肥（hyponex: hyponex Japan），但没有可靠的。Mathiola incana ‘Maihime’、Dianthus hybrida ‘Dai-ichi Kanzaki Siranui’、Dianthus caryophyllus ‘F1 Monark Scarlet’等已报道对DIF有反应，因此我们对这三个品种进行了比较。这些种子在塞盘中播种，并在生长室中以恒定的+5,0，-5 DIF发芽。每隔3 ~ 4天测量一次下胚轴长度和株高。塞盘法比培养皿法植株生长速度快，控苗容易。在观察中期存在DIF效应，但我们不能确定对DIF的反应是否判断为下胚轴长度。在第一次培养皿实验中，DIF从子叶开口开始供应。第二次试验开始于发芽期。因此。下胚轴长度生长对DIF的响应不明显。未来我们需要对这一点进行进一步的研究，并且我们需要研究在下胚轴早期萌发阶段或仅作为成虫的植物对DIF有反应的植物。

Some species of annual flowers were tested. To name a few, Mathiola incana(common stock), Dianthus hybrida(hybrid pink), Dianthus caryophyllus(carnation), Antirrhium majus(snapdraragon), Petunia hybrida(petunia) , Viola X wittrockiana(pansy) seedling were used. Seed were germinated on filter papers in growth chambers kept constantly positive(+5), zero(0), negative(-5) DIF.(DIF is defind as the mathematical DIF-ference between the day temperature and the night temperature, although average temperature is the same). A month later lengths of the hypocotyls were measured. The effect of DIF on hypocotyl length was not clear noticeably. The control seedlings in the petri dishes were not uniform therefore this method was regarded as unsuitable. The next year, liquid fertilization (hyponex : Hyponex Japan) was supplied but there was no reliable.Mathiola incana 'Maihime' , Dianthus hybrida 'Dai-ichi Kanzaki Siranui' Dianthus caryophyllus 'F1 Monark Scarlet' had been reported of response on DIF, so we concentrated on these three species in order to compare our technique. These were seeded in plug trays and germinated in the growth chambers as constantly +5, 0, -5 DIF.Hypocotyl length and plant height were measured every three or four days. Plant growth rate was faster when plug trays were used and seedling control was easier than in the petri dish. In the middle of observation there was a DIF effect, but we could not decided wheather the responce to DIF was judged hypocotyl length or not. In the first petri dish experiment, DIF was supply from opening of cotyledon onward. The 2nd experiment commenced at the germination stage. Consequently. the hypocotyl length growth response to DIF was not clear. In the future we need to research this point, morever we need to investigate plants that response to DIF at the hypocotyl early germenation stage or only as an adult plant.