Development of growth and flowering control of cut flower by DIF

对一些品种的一年生花卉进行了测试。仅举几个例子，马硫拉(普通苗)、石竹(杂交粉色)、香石竹(康乃馨)、马尾松(Snapdraragon)、矮牵牛(Petunia)、紫花地丁(Viola X Wittrock Iana)(三色)幼苗。种子在生长室的滤纸上萌发，保持恒定的正(+5)、零(0)、负(-5)DIF。(DIF是昼夜温度之间的数学DIF，虽然平均温度相同)。一个月后，测量了下胚轴的长度。DIF对下胚轴长度的影响不明显。培养皿内对照苗不均匀，认为该方法不宜采用。第二年，提供了液体受精，但没有可靠的方法。据报道，马硫拉的‘Maihime’、杂交的‘Dai-ichi Kanzaki Siranui’、香石竹的‘F1 Monark Scarlet’对DIF有反应，所以我们集中在这三个品种上，以便比较我们的技术。以+5，0，-5DIF的方式在生长室中萌发，每隔三四天测量下胚轴长度和株高。采用塞式托盘时，植株生长速度较快，易于进行育苗控制。在观察过程中出现了DIF效应，但不能确定对DIF的反应是否为下胚轴长度。在第一次培养皿实验中，从子叶开放开始供应DIF。第二次试验从萌发期开始。因此。下胚轴长度对DIF的生长反应尚不清楚。今后我们还需要研究这一点，更需要研究在下胚轴早期胚芽阶段或仅作为成年植株对DIF反应的植物。

Some species of annual flowers were tested. To name a few, Mathiola incana(common stock), Dianthus hybrida(hybrid pink), Dianthus caryophyllus(carnation), Antirrhium majus(snapdraragon), Petunia hybrida(petunia) , Viola X wittrockiana(pansy) seedling were used. Seed were germinated on filter papers in growth chambers kept constantly positive(+5), zero(0), negative(-5) DIF.(DIF is defind as the mathematical DIF-ference between the day temperature and the night temperature, although average temperature is the same). A month later lengths of the hypocotyls were measured. The effect of DIF on hypocotyl length was not clear noticeably. The control seedlings in the petri dishes were not uniform therefore this method was regarded as unsuitable. The next year, liquid fertilization (hyponex : Hyponex Japan) was supplied but there was no reliable.Mathiola incana 'Maihime' , Dianthus hybrida 'Dai-ichi Kanzaki Siranui' Dianthus caryophyllus 'F1 Monark Scarlet' had been reported of response on DIF, so we concentrated on these three species in order to compare our technique. These were seeded in plug trays and germinated in the growth chambers as constantly +5, 0, -5 DIF.Hypocotyl length and plant height were measured every three or four days. Plant growth rate was faster when plug trays were used and seedling control was easier than in the petri dish. In the middle of observation there was a DIF effect, but we could not decided wheather the responce to DIF was judged hypocotyl length or not. In the first petri dish experiment, DIF was supply from opening of cotyledon onward. The 2nd experiment commenced at the germination stage. Consequently. the hypocotyl length growth response to DIF was not clear. In the future we need to research this point, morever we need to investigate plants that response to DIF at the hypocotyl early germenation stage or only as an adult plant.