Development of growth and flowering control of cut flower by DIF

DIF 切花生长和开花控制的研究进展

• 批准号: 09660023
• 负责人: OHKAWA Kiyoshi
• 金额: $ 2.11万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660023/
• 关键词: DIF; Hypocotyl; Cut flower; Growth retardant; わい化剤; グロースリターダント; 切り花

Some species of annual flowers were tested. To name a few, Mathiola incana(common stock), Dianthus hybrida(hybrid pink), Dianthus caryophyllus(carnation), Antirrhium majus(snapdraragon), Petunia hybrida(petunia) , Viola X wittrockiana(pansy) seedling were used. Seed were germinated on filter papers in growth chambers kept constantly positive(+5), zero(0), negative(-5) DIF.(DIF is defind as the mathematical DIF-ference between the day temperature and the night temperature, although average temperature is the same). A month later lengths of the hypocotyls were measured. The effect of DIF on hypocotyl length was not clear noticeably. The control seedlings in the petri dishes were not uniform therefore this method was regarded as unsuitable. The next year, liquid fertilization (hyponex : Hyponex Japan) was supplied but there was no reliable.Mathiola incana 'Maihime' , Dianthus hybrida 'Dai-ichi Kanzaki Siranui' Dianthus caryophyllus 'F1 Monark Scarlet' had been reported of response on DIF, so we concentrated on these three species in order to compare our technique. These were seeded in plug trays and germinated in the growth chambers as constantly +5, 0, -5 DIF.Hypocotyl length and plant height were measured every three or four days. Plant growth rate was faster when plug trays were used and seedling control was easier than in the petri dish. In the middle of observation there was a DIF effect, but we could not decided wheather the responce to DIF was judged hypocotyl length or not. In the first petri dish experiment, DIF was supply from opening of cotyledon onward. The 2nd experiment commenced at the germination stage. Consequently. the hypocotyl length growth response to DIF was not clear. In the future we need to research this point, morever we need to investigate plants that response to DIF at the hypocotyl early germenation stage or only as an adult plant.

对几种一年生花卉进行了试验。举几个例子，使用了Mathiola incana（普通原种）、Dianthus hybrida（杂交粉红色）、Dianthus caryophyllus（康乃馨）、Antirrhium majus（金鱼草）、Petunia hybrida（矮牵牛）、Viola X wittrockiana（三色堇）幼苗。使种子在生长室中的滤纸上萌发，所述生长室保持恒定阳性（+5）、零（0）、阴性（-5）DIF。(DIF定义为白天温度和夜间温度之间的数学差异，尽管平均温度相同）。一个月后测量下胚轴的长度。DIF对下胚轴长度的影响不明显。培养皿中的对照幼苗不均匀，因此认为该方法不适用。第二年，采用了液体施肥（Hyponex：Hyponex Japan），但没有可靠的结果，因此我们集中研究了这三个品种，以比较我们的技术。将这些种子接种在穴盘中，并在生长室中以恒定+5，0，-5DIF发芽。每三或四天测量下胚轴长度和植物高度。植物生长速度更快时，使用穴盘和幼苗控制是比在培养皿中更容易。在观察的中期，有一个DIF效应，但我们不能确定是否对DIF的反应是判断下胚轴长度。在第一个培养皿实验中，DIF从子叶打开开始供应。第二个实验在发芽阶段开始。因此。下胚轴长度对DIF的生长反应尚不清楚。今后我们需要进一步研究这一点，尤其是研究在下胚轴萌发早期或仅在成株期对DIF有反应的植物。