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FUNCTION OF PLANT GROWTH PROMOTING FUNGI AND THEIR CONTROL OF SOIL-BORNE DISEASES

植物促生真菌的作用及其对土传病害的防治

基本信息

批准号：
09660044
负责人：
HYAKUMACHI Mitsuro
金额：
$ 1.79万
依托单位：
GIFU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660044/
关键词：
PLANT GROWTH PROMOTING FUNGI INDUCED RESISTANCE ANTHRACNOSE BACTERIAL ANGULARLEAF SPOT FUSARIUM WILT SOIL-BORNE DISEASES CUCUMBER

项目摘要

Plant growth promoting fungi (PGPF) exhibited the ability of plant growth promotion as well as disease suppression. Some PGPF isolates were also found capable of inducing systemic protection in cucumber against Colletotrichum orbiculare, the anthracnose pathogen. Cucumber plants even treated with culture filatrates (CFs) of PGPF induced a systemic resistance against this disease. Roots treated with CFs induced lignification at the points of attemped penetration by the pathogen indicating that elicitor substance(s) existed in the CFs. The elicitor activity of CFs was evaluated by the chemiluminescence assay using cucumber fruit disks. The emission of chemiluminescence from cucumber fruit disks treated with CFs was determined using ARGUS-50. The CFs of PGPF elicited conspicuous superoxide generation. The chemiluminescence activity of the CF of Penicillium GP17-2 was extremely high, and its intensity was almost 100 X that of other isolates. The activity was not lost after treatment with protease or autoclaving or after removal of lipid. The dialyzed CF fraction containing substances of MW.more than 2,000,000 was highly effective. This fraction could induce conspicuous lignification in the epidermal tissues of cucumber hypocotyl. Both lignification and superoxide generation are thought to be intimately related with the expression of induced resistance.

植物生长促进剂(PGPF)具有促进植物生长和抑制病害的作用。一些PGPF菌株还能诱导黄瓜对炭疽病菌产生系统保护作用。黄瓜植株即使用PGPF的培养物(CFs)处理，也诱导了对该病的系统抗性。CFS处理的根在病原菌试图穿透的点诱导木质化，表明CFS中存在激发子物质(S)。以黄瓜果盘为试材，采用化学发光法测定了CFS的激发子活性。用Argus-50测定了CFS处理的黄瓜果盘的化学发光。PGPF的CFs产生了明显的超氧化物歧化产物。青霉菌GP17-2胞外纤维膜的化学发光活性极高，其发光强度几乎是其他菌株的100倍。经酶处理、高压灭菌或去脂处理后，其活性不会丧失。透析后的CF组分的相对分子质量大于2,000,000，是高效的。该组分能诱导黄瓜下胚轴表皮组织发生明显的木质化。木质化和超氧化物的产生都被认为与诱导抗性的表达密切相关。