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Molecular Mechanisms of Insect Immunity

昆虫免疫的分子机制

基本信息

批准号：
09660092
负责人：
MORISHIMA Isao
金额：
$ 2.11万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660092/
关键词：
antibacterial protein cecropin lysozyme attacin peptidoglycan insect immunity silkworm Eri-silkworm 生体防御

项目摘要

(1)Minimum structure of peptidoglycan required for the induction of antibacterial proteins.Peptidoglycan fragments which differ in their peptide bridge structure or the chain length were prepared and tested for the inducing ability of antibacterial activity in the silkworm, Bombyx mori. The results indicated the silkworm recognized some definite structure of peptidoglycan, and the minimum structure was determined.(2)Isolation and characterization of peptidoglycan recognition protein.A peptidoglycan binding protein was isolated from immunized hemolymph of the silkworm larvae. The protein had a molecular weight of 37kd, and the N-terminal amino acid sequence so far determined had no homology to known proteins. The binding specificities and other characteristics of the protein showed the protein was the most possible for the candidate of the peptidoglycan recognition protein involved in the induction of antibacterial protein genes.(3)Signal transduction system involved in the induction of … More antibacterial protein genes.Testing various inhibitors of the signal transduction for the effect on the induction of antibacterial genes by peptidoglycan, eicosanoids were found to be involved in the induction of the genes.(4)Cloning and analysis of the promoter of cecropin genes.Two cecropin A gene were cloned by screening the silkworm genome. A NF-κB binding motif was found in the promoter region of the two genes. The protein which specifically bound to the motif was detected only in the nuclear extract from immunized fat body.(5)Detection and cloning of the gene induced by bacterial infection in the Eri-silkworm.By means of differential display method, several bacteria-induced genes including cecropin and lysozyme were isolated from immunized fat body of the Eri-silkworm, Samia cynthia ricini.(6)Protein isolation and cloning of attacin cDNA from Eri-silkworm.Attacin, an antibacterial protein, was isolated from immunized hemolymph of the Eri-silkworm. The DNA fragment synthesized according to the N-terminal amino acid sequence of attacin was used as a probe, and the cDNA was cloned and sequenced. The sequence had high homology to the attacin from cecropia silkmoth. Less

（1）抗菌蛋白诱导所需的最小肽聚糖结构制备了不同肽桥结构或不同链长的肽聚糖片段，并测试了其对家蚕抗菌活性的诱导能力。结果表明，家蚕识别了肽聚糖的某些结构，并确定了肽聚糖的最小结构。（2）肽聚糖识别蛋白的分离与鉴定从家蚕幼虫免疫后的血淋巴中分离到一种肽聚糖结合蛋白。该蛋白的分子量为37 kd，N端氨基酸序列与已知蛋白没有同源性。结合特异性和其他特性表明该蛋白最有可能是参与抗菌蛋白基因诱导的肽聚糖识别蛋白的候选蛋白。（3）参与诱导的信号转导系统 ...更多信息测试信号转导的各种抑制剂对肽聚糖诱导抗菌基因的影响，发现类花生酸参与基因的诱导。（4）天蚕素基因启动子的克隆与分析通过对家蚕基因组的筛选，克隆了两个天蚕素A基因。在这两个基因的启动子区均存在NF-κB结合基序。免疫脂肪体细胞核提取物中仅检测到与该基序特异性结合的蛋白。（5）蓖麻蚕细菌感染诱导基因的检测与克隆利用差异显示技术，从蓖麻蚕免疫后的脂肪体中分离到抗菌肽和溶菌酶等细菌感染诱导基因。（6）蓖麻蚕Attacin蛋白的分离与克隆从蓖麻蚕免疫血淋巴中分离到一种抗菌蛋白Attacin。根据attacin的N-末端氨基酸序列合成的DNA片段作为探针，克隆并测序了其cDNA。该序列与家蚕抗菌素具有较高的同源性。少