Antibacterial protein induced in insect

昆虫中诱导的抗菌蛋白

• 批准号: 60560095
• 负责人: MORISHIMA Isao
• 金额: $ 1.28万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60560095/
• 关键词: Antibacterial protein; Silkworm; Bombytoxin; Bombyx mor:; 家蚕

Injection of live or heat-killed bacteria into larvae of the silkworm, Bombyx mori induced antibacterial activities in the hemolymph. Although there was no specificity of injected bacteria for the induction of antibacterial activity, yeast cells, fungus spores and saline alone were not effective as inducing agent.The antibacterial activities were separated into at least six bands on native poly-acrylamide gel electrophoresis, which were sensitive to trypsin. The antibacterial activities were separated from lysozyme activity on CM-cellulose column chromatography. Two of these proteins (AP-A and AP-B) were purifies to apparent homogenity. They were heat-stable and showed characteristic properties of cecropins. The proteins repressed growth of various bacteria at a concentration of 0.1 ug/ml.

将活细菌或热灭活细菌注射到家蚕幼虫体内，可诱导血淋巴产生抗菌活性。虽然注射的细菌对于诱导抗菌活性没有特异性，但酵母细胞、真菌孢子和盐水单独作为诱导剂并不能有效。在对胰蛋白酶敏感的非变性聚丙烯酰胺凝胶电泳上，抗菌活性被分成至少六个条带。通过CM-纤维素柱色谱将抗菌活性与溶菌酶活性分开。其中两种蛋白质（AP-A 和 AP-B）经过纯化，具有明显的同质性。它们具有热稳定性并表现出天蚕素的特征。浓度为 0.1 ug/ml 的蛋白质可抑制多种细菌的生长。

项目成果

Morishima, Isao; Ueno, Teruo; Shigeyuki Suginaka: "Induction and partial characterization of antibacterial protein in hemolymph of silkworm, Bombyx mori" Submitted for publication.

森岛勋；