Induction mechanism for antibacterial protein synthesis in insect.

昆虫抗菌蛋白合成的诱导机制。

• 批准号: 04660087
• 负责人: MORISHIMA Isao
• 金额: $ 1.34万
• 依托单位: TOTTORI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660087/
• 关键词: Antibacterial protein; Cecropin; Lyszyme; Peptdoglycan; Silkworm; cDNA; Induction mechanism; Bombyx mori

1. Variou baterial peptidoglycans (PG), which were different in peptide bridge structure or sugar-chain length, were tested for elicitor of antibacterial protein synthesis in the silkworm, Bombyx mori, and the minimum structure required for the induction was elucidated.2. The hemolymph from non-immunized larvae was found to contain high levels of lysozyme, and the activity increased 10-times after injection of PG.The roles of hemolymph lysozyme in the induction of antibacterial protein were clarified by kinetic studies with in vitro fat body culture system.3. cDNAs for cecropns A and B were coned and sequenced from immunized larval fat body. Using the cDNAs as probes, tissue specific expression of the cecropin genes was investigated.4. The gene for cecropin A was solated and the structure was analyzed. A novel NF-kappa B binding motif was fund in upstream region of the promoter. The finding suggests that NF-kappa B-like protein may be involved in the regulatin of immune gene expression in insect.

1.研究了不同肽桥结构和糖链长度的细菌肽聚糖（PG）对家蚕抗菌蛋白合成的诱导作用，并阐明了诱导所需的最低结构.未免疫幼虫的血淋巴中含有大量溶菌酶，注射PG后溶菌酶活性增加了10倍。通过体外脂肪体培养系统的动力学研究，阐明了血淋巴溶菌酶在抗菌蛋白诱导中的作用.从免疫的幼虫脂肪体中克隆出cecropns A和B的cDNA并测序。以cDNAs为探针，研究了天蚕素基因的组织特异性表达.克隆了天蚕素A基因，并对其结构进行了分析。在启动子上游发现了一个新的NF-κ B B结合基序。提示NF-κ B样蛋白可能参与昆虫免疫基因表达调控。

项目成果

Iketan,M.: "Induction of antibacterial protein synthesis by soluble peptidoglycan in isolated fat body" Insect.Biochem.Molec.Biol.,. 23. 913-917 (1993)

Iketan,M.：“通过分离脂肪体中的可溶性肽聚糖诱导抗菌蛋白合成”Insect.Biochem.Molec.Biol.,。

Morishima, I., Horiba T.and Yamano, Y.: "Lysozyme activity in immunized and non-immunized hemolymph during the development of the silkworm, Bombyx mori." Comp.Biochem.Physiol.(in press.).

Morishima, I.、Horiba T. 和 Yamano, Y.：“家蚕发育过程中免疫和非免疫血淋巴中的溶菌酶活性。”

Morishima, I., Yamada, K.and Ueno, T: "Bacterial peptdoglycan as elicitor of antibacterial protein synthesis in larvae of the silkworm, Bombyx mori" Insect Biochem.Mole.Biol.22. 363-367 (1992)

Morishima, I.、Yamada, K. 和 Ueno, T：“细菌肽聚糖作为蚕幼虫 Bombyx mori 抗菌蛋白合成的引发剂”Insect Biochem.Mole.Biol.22。

Morishima,I.: "Bacterial peptidoglycan as elicitor of anti-bacterial protein synthesis in larval of the silkworm." Insect Biochem.Molec.Biol.,. 22. 363-367 (1992)

Morishima,I.：“细菌肽聚糖作为蚕幼虫抗菌蛋白合成的诱导剂。”

Morishima,I: "Lysozyme activity in immunized and non-immunized nemolymph during the development of the silkworm." Comp.Biochem.Physiol.(印刷中). (1994)

Morishima, I：“蚕发育过程中免疫和非免疫淋巴液中的溶菌酶活性。”Comp.Biochem.Physiol.（出版中）。