Screening of New Biocatalysts and Its Application to the Synthesis of Biologically Active Compounds

本文综述了新型生物催化剂的筛选及其在生物活性化合物合成中的应用。1)（E）-3-氨基甲酰基-1-丙烯基硼酸在Suzuki-Miyaura偶联和酶反应中的关键作用：（3E，5E）-和（3E，5 Z）-6-苯基-3，5-己二烯酸的合成; 2）生物催化剂介导的高对映体富集的（R）-5-己二烯酸的有效制备; 3）从三-O-乙酰基-D-葡萄糖醛化学-酶促合成D-阿洛糖胺衍生物; 4）紫红红球菌IFO 15564介导的脂环腈和酰胺的水解：其立体选择性，以及用于动力学拆分和不对称化; 5）脂肪酶催化的神经酰胺的对映体拆分。

Results of screeing of new biocatalysts and its application to the synthesis of biologically active compounds are summalized as follows. 1) pivotal role of (E)-3-carbamoyl-1-propenylboronic acid in the combination of Suzuki-Miyaura coupling and enzyme reactions : synthesis of (3E,5E)- and (3E,5Z)-6-phenyl-3,5-hexadienoic Acid ; 2) biocatalyst-mediated efficient preparation of highly enantiomerically enriched (R)-5-hexanolide ; 3) A chemo-enzymatic synthesis of D-allosamine derivatives from Tri-O-acetyl-D-glucal ; 4) Rhodococcus rhodochrous IFO 15564-mediated hydrolysis of alicyclic nitriles and amides : its stereoselectivity, and the use for kinetic resolution and asymmetrization ; 5) lipase-catalyzed enantiomeric resolution of ceramides.