Screening of New Biocatalysts and Its Application to the Synthesis of Biologically Active Compounds

新生物催化剂的筛选及其在生物活性化合物合成中的应用结果总结如下。1)(E)-3-氨基甲酰基-1-丙烯基硼酸在Suzuki-Miyaura偶联反应和酶反应组合中的关键作用：(3E，5E)-和(3E，5Z)-6-苯基-3，5-己二烯酸的合成；2)生物催化剂介导的高对映体富集型(R)-5-己内酯的高效制备；3)三-O-乙酰-D-醛的化学酶催化合成D-异丙三胺衍生物；4)红球菌IFO 15564-介导的脂环腈和酰胺的糖基化：其立体电性，并用于动力学拆分和不对称拆分；5)脂肪酶催化的金属陶瓷对映体拆分。

Results of screeing of new biocatalysts and its application to the synthesis of biologically active compounds are summalized as follows. 1) pivotal role of (E)-3-carbamoyl-1-propenylboronic acid in the combination of Suzuki-Miyaura coupling and enzyme reactions : synthesis of (3E,5E)- and (3E,5Z)-6-phenyl-3,5-hexadienoic Acid ; 2) biocatalyst-mediated efficient preparation of highly enantiomerically enriched (R)-5-hexanolide ; 3) A chemo-enzymatic synthesis of D-allosamine derivatives from Tri-O-acetyl-D-glucal ; 4) Rhodococcus rhodochrous IFO 15564-mediated hydrolysis of alicyclic nitriles and amides : its stereoselectivity, and the use for kinetic resolution and asymmetrization ; 5) lipase-catalyzed enantiomeric resolution of ceramides.