Development of apoptotic cell detection using anti-DNaseγ antibody

使用抗 DNaseγ 抗体开发凋亡细胞检测

• 批准号: 09557138
• 负责人: HASHIZUME Kohei
• 金额: $ 7.36万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557138/
• 关键词: apoptosis; Dnaseγ; 小児癌; 神経芽細胞腫; DNA断片化; 診断法; エンドヌクレアーゼ

In this report we demonstrated that the mechanism of rat neuroblastoma cell line B-50 death after differentiation to neuron cell are apoptosis, and Dnaseγ are closely related to the induction of apoptosis of the cell line. And also we demonstrated that in mice genomic expression of Dnaseγ in the brain were strongly detected from 14 to 16 fetal days. These genomic expression were also detected in neuroblastoma cell line B-50 when they differentiated into neuronal cells.Dnaseγ might be a good marker of prognosis expectation in pediatrinc cancer and detecting the Dnaseγ expression in the tumor cells using an anti Dnaseγ antibody are useful for expecting the prognosis of pediatric cancer.

本研究证实大鼠神经母细胞瘤细胞系B-50分化为神经元细胞后死亡的机制为细胞凋亡，而Dnaseγ与诱导细胞凋亡密切相关。我们还证明，小鼠大脑中dna γ的基因组表达在14到16个胎儿日被强烈检测到。这些基因表达在神经母细胞瘤细胞系B-50分化为神经细胞时也被检测到。Dnaseγ可能是儿童肿瘤预后预期的良好标志物，使用抗Dnaseγ抗体检测肿瘤细胞中Dnaseγ的表达有助于预测儿童癌症的预后。

项目成果

D. Shiolawa, M. Hirai, and S. Tanuma: "DNA cloning of human Dnase g chromosomal localization of its gene and enzymatic properties of recombinant protein."Apoptosis. 3. 89-95 (1998)

D. Shiolawa、M. Hirai 和 S. Tanuma：“人 Dnase g 基因的 DNA 克隆及其基因的染色体定位和重组蛋白的酶学特性。”细胞凋亡。

S. Tanuma.: "The effect of long-chain fatty acids on neuronal cell death. :"J, Japan Oil Chem. Soc. 46. 1087-1093 (1997)

S. Tanuma.：“长链脂肪酸对神经元细胞死亡的影响。：”J，日本石油化学。

P.Shiokawa,et al.: "DNA cloning of human DNaseγ,chromosomal localization of its gene and enzymatic properties of recombinant protein"Apoptosis. 3. 89-95 (1998)

P. Shiokawa 等人：“人 DNaseγ 的 DNA 克隆、其基因的染色体定位和重组蛋白的酶学特性”Apoptosis 3. 89-95 (1998)。

田沼靖一: "臨床免疫" アポトーシスの分子機構, 183 (1997)

Seiichi Tanuma：《临床免疫学》细胞凋亡的分子机制，183（1997）

K.Nishimura,et al.: "Presence of DNaseγ-like endonuclease in nuclei of neuronal differentiated pC12 cells." Apoptosis. 3. 97-103 (1998)

K. Nishimura 等人：“神经元分化的 pC12 细胞核中存在 DNaseγ 样核酸内切酶。” 3. 97-103 (1998)