Development of apoptotic cell detection using anti-DNaseγ antibody

本研究证实了大鼠神经母细胞瘤B-50细胞分化为神经细胞后死亡的机制为细胞凋亡，DNA酶γ与诱导细胞凋亡密切相关。我们还证明，在小鼠的大脑中，DNA酶γ在胚胎14-16天的基因组中有强烈的表达。在神经母细胞瘤细胞系B-50分化为神经细胞时也检测到这些基因组的表达。DNA酶γ可能是预测儿童肿瘤预后的良好指标，用抗DNA酶γ抗体检测肿瘤细胞中DNA酶γ的表达有助于预测儿童癌症的预后。

In this report we demonstrated that the mechanism of rat neuroblastoma cell line B-50 death after differentiation to neuron cell are apoptosis, and Dnaseγ are closely related to the induction of apoptosis of the cell line. And also we demonstrated that in mice genomic expression of Dnaseγ in the brain were strongly detected from 14 to 16 fetal days. These genomic expression were also detected in neuroblastoma cell line B-50 when they differentiated into neuronal cells.Dnaseγ might be a good marker of prognosis expectation in pediatrinc cancer and detecting the Dnaseγ expression in the tumor cells using an anti Dnaseγ antibody are useful for expecting the prognosis of pediatric cancer.