Development of high-resolution DNA typing methods for HLA class 1 genes.
开发 HLA 1 类基因的高分辨率 DNA 分型方法。
基本信息
- 批准号:09557215
- 负责人:
- 金额:$ 7.62万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1999
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1. Screening of variations at HLA-A, -B, and -C loci followed by nucleotide sequencing elucidated a number of new alleles including HLA-B22, -B27, -Cw2, and several non-expressed (null) alleles. Consequently, we could identify most, if not all, of the HLA class I alleles existing in the Japanese population.2. DNA-based typing kits for HLA-A and -B loci at the intermediate resolution were established using the PCR-MPH method. Also, DNA-based typing kits for HLA-C locus as well as -A2, -A26, -B15, and -B40 groups at the sequence-level resolution were established using the PCR-MPH method. Thus, most of the HLA class I alleles in Japanese can be detected using these PCR-MPH kits. In addition, DNA-based typing methods for HLA-A and B loci at the sequence-level resolution, suitable for small number of samples, were established using the PCR-RFLP method.3. Using the above methods, we performed sequence-level typing for HLA-A, B, and C loci in Japanese population samples, and reported allele and haplotype frequenecies. Moreover, we investigated the diversity of alleles and haplotypes of HLA-B61 and -B17 groups in East Asian populations.
1.筛选HLA-A、-B和-C位点的变异,然后进行核苷酸测序,阐明了许多新的等位基因,包括HLA-B22、-B27、-Cw 2和几个未表达(无效)等位基因。因此,我们可以确定大多数,如果不是全部,存在于日本人口中的HLA I类等位基因。应用聚合酶链反应-多聚体酶切(PCR-MPH)技术,建立了HLA-A、-B基因座中分辨率DNA分型试剂盒。同时,采用PCR-MPH方法建立了HLA-C位点及-A2、-A26、-B15、-B40四组DNA分型试剂盒。因此,日本人的大多数HLA I类等位基因可以使用这些PCR-MPH试剂盒检测。此外,利用PCR-RFLP方法,建立了适合于小样本量的HLA-A、B基因座的DNA分型方法.使用上述方法,我们在日本人群样本中对HLA-A、B和C位点进行了序列水平分型,并报告了等位基因和单倍型频率。此外,我们还调查了东亚人群中HLA-B61和-B17等位基因和单倍型的多样性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Chida S, et al.: "Molecular analyses of the possible RNA-binding protein gene located in the human leukocyte antigen (HLA)-DR subregion."Genes and Development. 240. 125-132 (1999)
Chida S 等人:“对位于人类白细胞抗原 (HLA)-DR 亚区的可能的 RNA 结合蛋白基因进行分子分析。”基因与开发。
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- 影响因子:0
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Wang H, Tokunaga K. Akaza T, Tadokoro K, Shibata Y, and Juji T: "Identification of HLA-C alleles using PCR-single-strand-conformation polymorphism and direct sequencing."Tissue Antigens. 49. 134-140 (1997)
Wang H、Tokunaga K. Akaza T、Tadokoro K、Shibata Y 和 Juji T:“使用 PCR 单链构象多态性和直接测序鉴定 HLA-C 等位基因。”组织抗原。
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Nagao F, Yabe T, Lin L, Tokunaga K, Juji T, and Okumura K: "Japanese population does not manifest significant association between low NK activity and HLA-B(C) locus homozygosity."Hum. Immunol. 53. 17-22 (1997)
Nagao F、Yabe T、Lin L、Tokunaga K、Juji T 和 Okumura K:“日本人群并未表现出低 NK 活性与 HLA-B(C) 位点纯合性之间的显着关联。”嗯。
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Furukawa H, Yabe T, Akaza T, Tadokoro K, Tohma S, Inoue T, Tokunaga K, Yamamoto K, Geraghty D, and Juji T: "Cell surface expression of HLA-E molecules on PBMC from a TAP1-deficient patient."Tissue Antigens. 53(3). 292-295 (1999)
Furukawa H、Yabe T、Akaza T、Tadokoro K、Tohma S、Inoue T、Tokunaga K、Yamamoto K、Geraghty D 和 Juji T:“来自 TAP1 缺陷患者的 PBMC 上 HLA-E 分子的细胞表面表达。”
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Hasegawa,T.: "Novel HLA-B27 allele(B^*2711)encoding an antigen reacting with both B27 and B40 specific antisera." Tissue Antigens. 49・6. 649-652 (1997)
Hasekawa, T.:“新型 HLA-B27 等位基因 (B^*2711) 编码与 B27 和 B40 特异性抗血清反应的抗原。”49·6 (1997)。
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TOKUNAGA Katsushi其他文献
TOKUNAGA Katsushi的其他文献
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{{ truncateString('TOKUNAGA Katsushi', 18)}}的其他基金
Pharmacogenomics study of anti-tuberculosis drugs in the Asian population
亚洲人群抗结核药物的药物基因组学研究
- 批准号:
24406010 - 财政年份:2012
- 资助金额:
$ 7.62万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Networking for research on human genetics of Tuberculosis
结核病人类遗传学研究网络
- 批准号:
21406006 - 财政年份:2009
- 资助金额:
$ 7.62万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Polymorphisms and disease associations of receptor gene families for class I antigens
I类抗原受体基因家族的多态性和疾病关联
- 批准号:
13470506 - 财政年份:2001
- 资助金额:
$ 7.62万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of new SNP typing technologies for searching disease-associated genes.
开发用于搜索疾病相关基因的新 SNP 分型技术。
- 批准号:
12557237 - 财政年份:2000
- 资助金额:
$ 7.62万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Genes associated with rheumatic and diffuse collagen diseases
与风湿性和弥漫性胶原病相关的基因
- 批准号:
12204002 - 财政年份:2000
- 资助金额:
$ 7.62万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Study on interactions between infectious diseases and host genetic factors in East Asia and Oceania
东亚和大洋洲传染病与宿主遗传因素相互作用的研究
- 批准号:
11440248 - 财政年份:1999
- 资助金额:
$ 7.62万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
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