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Establishment of Supporting System for evaluating Biological Environments based on Protistan DNA nucleotide sequences

基于原生生物DNA核苷酸序列的生物环境评价支撑系统的建立

基本信息

批准号：
09554025
负责人：
KITAZATO Hiroshi
金额：
$ 8.26万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09554025/
关键词：
DNA Protista biological environments assessment system database diversity adaptive morphology Foraminifera 環境調査珪藻

项目摘要

In order to establish a support system for assessing marine biological environments, a genetic database of protistan species, which are commonly used as environmental proxies, was constructed for ribosomal DNA sequences. DNA analyses. were carried out for LSU, SSU and ITS sequence regions of ribosomal DNA. Relationships between species and ambient environments were compiled, both from publications and unpublished theses of Shizuoka University, to provide ecological or morphological constraints for the database.Samples for analyses were collected from seven innerbays from north to south along the Japanese Islands. Six species of benthic foraminifera (Protista) were selected for analysis. All are commonly used as proxies for dysaerobic environment or eutrophicated condition within the inner bay environment.The database was constructed for DNA sequences of these species. Sequence data for each species were listed, together with intraspecific and/or interspecific divergences, as a dataset. … More Physiological, ecological and morphological responses to ambient environmental changes were compiled to characterize each species as a specific environmental proxy. For instance, Trochammina hadai Uchio, which dwells in eutrophic environments of brackish bays, changes its test morphology in relation to the dissolved oxygen concentration at the sediment-water interface. This species reproduces at a water temperature of 10〜18 ℃, salinity of 25〜30 ‰, and dissolved oxygen concentration of 6〜8 ml/L. Sequence data for this species are sufficiently unique to discriminate from similar taxa. LSU rDNA sequences show only 0.1% difference between distributional areas. The ITS region, where substitution occurs very frequent, shows 2.0% of sequence differences.Sequence divergence tends to be smaller in highly polluted area of the sea, where heavy deposition of organic carbon takes place, than in less-polluted area. This tendency can be used as a good proxy for assessing precise environmental changes in response to pollution. Less

为了建立海洋生物环境评价的支持体系，构建了作为环境指标的原生生物物种核糖体DNA序列遗传数据库。DNA分析。对核糖体DNA的LSU、SSU和ITS序列区进行检测。从静冈大学的出版物和未发表的论文中编译物种与环境之间的关系，为数据库提供生态或形态约束。用于分析的样本是从沿日本群岛从北到南的七个内湾收集的。选取6种底栖有孔虫（原生虫）进行分析。所有这些通常被用作内湾环境中缺氧环境或富营养化条件的代理。建立了这些物种的DNA序列数据库。每个物种的序列数据连同种内和/或种间差异作为一个数据集被列出。对周围环境变化的生理、生态和形态反应进行了汇编，以表征每个物种作为特定环境代理的特征。例如，生活在咸淡海湾富营养化环境中的Trochammina hadai Uchio会根据沉积物-水界面的溶解氧浓度改变其测试形态。水温为10 ~ 18℃，盐度为25 ~ 30‰，溶解氧浓度为6 ~ 8 ml/L。该物种的序列数据具有足够的独特性，可以与相似的分类群进行区分。LSU rDNA序列在分布区域之间的差异仅为0.1%。ITS区域，替换发生非常频繁，显示2.0%的序列差异。在有机碳大量沉积的高污染海域，层序分化程度往往比低污染海域小。这种趋势可以用来作为一个很好的代理，以评估对污染作出反应的精确环境变化。少