Possibility of the research on ancient DNA for marine eukaryotes

海洋真核生物古DNA研究的可能性

• 批准号: 23654179
• 负责人: KITAZATO Hiroshi
• 金额: $ 2.41万
• 依托单位: Japan Agency for Marine-Earth Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23654179/
• 关键词: 系統; 進化; 多様性; 深海; 堆積物; 化石DNA; 海生生物; DNA解析; Bioinformatics

Fossilized DNA are sometimes preserved in anaerobic marine strata. This study is attempted to find fossilised DNA from deep-sea drill core beneath sea floor. Genome-level evolutionary biology for marine organisms are probably made with fossil DNA.We tried to amplify DNA fragments from core samples collected by deep-sea drill vessel CHIKYU. We found DNA fragments with 100s to 1,000s bp from the core. Amounts of DNA are more than 0.6ng/ul/mg dry weighted sediments. Two to four marine eukaryotic groups were identified in the DNA fragments. We believe that we have gotten a proof that fossilized DNA are certainly preserved in anaerobic environmental conditions which are created in the deep-sea sediments.

化石DNA有时被保存在厌氧的海洋地层中。这项研究试图从海底深处的钻芯中找到DNA化石。海洋生物基因组水平的进化生物学可能是由化石DNA构成的。我们试图从深海钻探船CHIKYU收集的岩心样本中扩增DNA片段。我们发现了距离核心100到1000个bp的DNA片段。干重沉积物中DNA含量大于0.6ng/ul/mg。在DNA片段中鉴定出2 ~ 4个海洋真核生物群。我们相信，我们已经得到了证据，证明化石DNA肯定保存在深海沉积物中产生的厌氧环境条件下。

项目成果

独立行政法人海洋研究開発機構 地球深部探査センター

日本海洋地球科学技术机构深地地球探索中心

日本の海はなぜ豊かなのか

为什么日本的海洋如此丰富？

• 发表时间: 2012
• 作者: Xue-fang Yu;Hiroshi Ushiyama;and Koichi Yamashita;H.Hidaka;北里洋
• 通讯作者: 北里洋