Microarray-based identification and quantification of molecular biomarkers for hydrogen-metabolizing microorganism communities to assess bioremediation potential for reductive dechlorination at contaminated sites

基于微阵列的氢代谢微生物群落分子生物标志物的鉴定和定量，以评估污染场地还原脱氯的生物修复潜力

• 批准号: 88388908
• 负责人: Dr. Svenja Lohner
• 金额: --
• 依托单位: Department of Chemical Engineering
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/88388908?language=en
• 关键词: Microarray; based; identification; quantification; molecular

Reductive dehalogenation of chlorinated organic compounds is often limited by electron donor, i.e. hydrogen availability. The development of biomarkers indicating hydrogen flux between hydrogen metabolizing microorganisms and the expression level of hydrogenase genes is promising to assess groundwater contaminant degradative potential. To identify significant biomarkers, in the proposed research first microarray-based tools will be developed to assess abundance and expression levels of hydrogenases in pure and enriched cultures as well as in environmental field samples. Second, microcosm studies with hydrogen-metabolizing microorganisms will be performed to assess kinetics of hydrogen-relevant transformation processes in correlation with quantity and expression levels of hydrogenase genes. Third, hydrogenase genes identified to be good expression markers by microarray analysis will be additionally quantified and quantitative data will be correlated with microbial activity. Derived correlations will result in novel tools to assess biodegradative potential for reductive dehalogenation at contaminated sites leading to a more effective management of bioremediation strategies.

氯代有机化合物的还原脱卤通常受到电子供体（即氢的可用性）的限制。开发指示氢代谢微生物之间的氢通量和氢酶基因表达水平的生物标志物有望评估地下水污染物的降解潜力。为了确定重要的生物标志物，在拟议的研究中，将开发第一个基于微阵列的工具，以评估纯和富集培养物以及环境现场样品中氢化酶的丰度和表达水平。其次，将进行氢代谢微生物的微观研究，以评估与氢化酶基因的数量和表达水平相关的氢相关转化过程的动力学。第三，通过微阵列分析鉴定为良好表达标志物的氢化酶基因将被另外定量，并且定量数据将与微生物活性相关。衍生的相关性将导致新的工具，以评估在污染场地的还原脱卤生物降解潜力，从而更有效地管理生物修复策略。