Identification of susceptibility gene of diabetes mellitus y QTL analysis in the genetic modified mice.

I型和II型糖尿病是一种常见病，也是一个主要的公共卫生问题。为了确定糖尿病的易感基因，我们进行了全基因组数量性状基因座(QTL)分析，以C57BL6/KSJ背景下的db小鼠或NOD-RGP-IL-10TG背景下的NOD-RGP-IL-10TG为研究对象。DB小鼠是人类II型糖尿病的动物模型，表现为高血糖、高胰岛素血症、胰岛素抵抗和肥胖。Db小鼠和非糖尿病C3H品系的F2杂交显示了与糖尿病相关的多种疾病，包括体重、血糖浓度、血浆胰岛素浓度和葡萄糖耐量。我们已经确定了几个与体重和糖耐量相关的QTL。NOD-RGP-IL-10 TG可增强自身免疫性糖尿病，但与C57BL/6杂交的F1小鼠均未出现糖尿病。NOD-RGP-IL-10TG与C57BL/6小鼠回交2次，糖尿病发生率为15%。与db小鼠相似，我们正在对n2代进行全基因组数量性状基因座(QTL)分析。

Type I and II diabetes is a common disease and a major public health problem. To identify the susceptibility gene to diabetes we performed genome-wide quantitative trait loci (QTL) analysis, using db mice in C57BL6/ksj background or NOD-RGP-IL-10 Tg in NOD background. db mouse is an animal model of type II diabetes in human exhibiting hyperglycemia, hyperinsulinemia, insulin resistance, and obesity. F2 intercross between db mice and non-diabetic C3H strain demonstrated a wide variety of diabetes related, including body weight, plasma glucose concentration, plasma insulin concentration, and glucose tolerance. We have identified several QTLs associated with body weight and glucose tolerance. NOD-RGP-IL-10 Tg enhance autoimmune diabetes, but none of the resulting F1 mice outbred to C57BL/6 mice show diabetes. N2 backcross between NOD-RGP-IL-10 Tg and C57BL/6 mice demonstrated 15% incidence of diabetes. Similar to db mice, we are performing genome-wide quantitative trait loci (QTL) analysis for N2 generation.