Disturbance of spermatogenesis and apoptosis

精子发生和细胞凋亡的干扰

• 批准号: 09470350
• 负责人: SASAKI Shoichi
• 金额: $ 7.3万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470350/
• 关键词: Disturbance of spermatogenesis; Apoptosis; Nuclear factor-kappa B; Rat; Testis; P53; NO; Calpain; Nuclear factor-kappaB; 造精障害; Nuclear Factor kappa B; N-acetyl-l-cystein

The spermatogenesis is hindered by various stresses. It is reported that the apoptosis is concerned in the spermatogenesis failure. Using the rat in each following experimental model, we examined the expression of the apoptosis and involvement of transcription factor nuclear factor-kappa B(NFκB) for the disorder of spermatogenesis. 【encircled1】 cryptorchidism (model rat of high temperature environment of testicles which fixed the testicles of the maturation rat in the peritoneal cavity and cryptorchidism rat made by administering Flutamide for the fetal stage 【encircled2】 torsion of thespermatic cord (unilateral and bilateral) 【encircled3】 the obstruction of seminal tract cord (unilateral and bilateral) 【encircled4】 the chemical castration (leuprolerin acetate 3mg/kg administration) 【encircled5】 diethylstilbesterol (500μg/body x 28days) administration 【encircled6】 testicular trauma cord (unilateral and bilateral)The testes of either model caused the atrophy of which was more significan … More t than the controls, and the expression of large number of apoptotic cells was recognized in the process. In the unilateral model of 【encircled2】,【encircled3】 and 【encircled6】, the apoptosis was also recognized in the contra-lateral tedticles, and it considered that the apoptosis was related with the crisis of the autoimmune orchitis. In 【encircled2】 the calpain which was the calcium-dependent cysteine protease increased, and calpain production and apoptosis expression were suppressed by the antioxidant. In 【encircled3】 the expression of the NOS increased with the increase in the apoptosis cell. In each model the conversion of NFκB to the nucleus and the expression of the p53 protein were observed with the apoptosis expression. In addition, the nucleus internal transportation of NFκB was suppressed, and the apoptosis expression was strengthened when the inhibitor of NFκB was administered in 【encircled4】 and 【encircled5】.From the above results, it was considered that NFκB had been activated in the normal rat testicular cells, and it might suppress the apoptosis. The suppression of the NFκB activity seemed to be concerned in the strong expression of the apoptosis in the disturbance of the spermatogenesis. Less

精子发生受到各种应激的阻碍。研究表明，细胞凋亡与精子发生障碍有关。采用以下各实验模型，检测睾丸细胞凋亡的表达及转录因子核因子-κ B（NFκB）在精子发生障碍中的作用。隐睾症（睾丸高温环境模型大鼠，将成熟大鼠的睾丸固定在腹腔内，隐睾大鼠通过给药氟啶用于胎儿期[包围2]精索扭转（单侧和双侧）[包围3]精道索阻塞（单侧和双侧）[圈4]化学阉割（醋酸亮丙瑞林3 mg/kg给药）[圈环5]己烯雌酚（500μg/体× 28天）给药[圈6]睾丸创伤索（单侧和双侧）两种模型的睾丸均引起萎缩， ...更多信息 与对照组相比，在此过程中发现了大量凋亡细胞的表达。在[encircled 2]、[encircled 3]和[encircled 6]的单侧模型中，对侧睾丸组织中也发现了凋亡，认为凋亡与自身免疫性睾丸炎的危象有关。在[encircled 2]中，钙依赖性半胱氨酸蛋白酶钙蛋白酶增加，抗氧化剂抑制钙蛋白酶的产生和凋亡表达。在[encircled 3]中，NOS的表达随着凋亡细胞的增加而增加。在各模型中观察NFκB B向核内的转化和p53蛋白的表达及细胞凋亡的表达。此外，在[encircled 4]和[encircled 5]中给予NFκB抑制剂后，NF κ B的核内转运受到抑制，凋亡表达增强，上述结果表明正常大鼠睾丸细胞中NFκB已被激活，可能抑制了凋亡。NFκB活性的抑制可能与精子发生障碍时细胞凋亡的强烈表达有关。少